FastQCFastQC Report
Tue 7 May 2024
EGAF00004692152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120216
Total Bases7.7 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31812.6460704066014507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.38929926132960674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.37682172090237576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.3235842150795235No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG3420.2844879217408664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.27284221734211755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2940.24455979237372727No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAA2870.23873694017435285No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.2129500232914088No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.21211818726292672No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.19964064683569574No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.1963133027217675No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.1954814666932854No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.18549943435150062No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.17052638583882346No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.16553536966793106No Hit
GTGCAACGTGGGGGCTGGAGGGTGGGAAAAAAAAAAAAAAAAAAAAAAAA1950.1622080255540028No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.1622080255540028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.1613761895255207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.15804884541159248No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.15056232115525386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1790.14889864909828973No Hit
GGTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGG1720.14307579689891528No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1720.14307579689891528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.13974845278498702No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1680.13974845278498702No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.13725294469954083No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAA1580.13143009250016635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.12976642044320225No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT1550.12893458441472017No Hit
GGTGCAACGTGGGGGCTGGAGGGTGGGAAAAAAAAAAAAAAAAAAAAAAA1470.12227989618686363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.12061622412989952No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.11978438810141745No Hit
GAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA1440.11978438810141745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.11895255207293538No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.11479337193052506No Hit
ACGTGGGGGCTGGAGGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.11479337193052506No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.11479337193052506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.113961535902043No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAA1290.10730684767418647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.10730684767418647No Hit
GTATCAACGCAGAGTACGGGAGGCAGCGGCCCAGTGAAATGCTTCCAACG1290.10730684767418647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.1064750116457044No Hit
GGGCTGGAGGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.10397950356025822No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.101483995474812No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)