FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004692153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226833
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT10950.4827339937310709No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.334166545432102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7190.3169732799019543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.2724471307084948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.26803860108537997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.2186630693064942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.21778136338187126No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.21381368672106793No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.20720089228639574No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4320.19044847971855947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.18956677379393652No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.1745777730753462No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.1719326553014773No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.1631155960552477No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.1604704782813788No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.1542985368090181No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.1516534190351492No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.14900830126128034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.1406320949773622No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.12696565314570632No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.12652480018339485No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.12652480018339485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.122997976484903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.11903029982409967No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.11903029982409967No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.11770774093716524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.11682603501254228No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2640.11638518205023078No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAG2630.11594432908791931No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.1141809172386734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.11329921131405044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.10977238761555859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.10756812280400117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.10007362244470602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)