FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156356
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT27501.7588068254496148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.36839008416690117No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT5300.3389700427230167No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.33257438153956353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.31530609634424006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.2590242779298524No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.215533781882371No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.21041725293560848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.21041725293560848No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC3160.2021028933971194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.20146332727877408No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2760.17652024866330682No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.1758806825449615No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.1758806825449615No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.1752411164266162No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.16372892629640054No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.1534958684028755No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.15221673616618486No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.15093760392949424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.14646064110107704No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.14006497991762387No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.13878584768093324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.1368671493258973No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.13111105426078948No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.13111105426078948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.12407582695899103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.12279669472230038No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG1910.12215712860395508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.11895929801222851No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.11831973189388319No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1840.11768016577553787No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.11640103353884725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.11512190130215662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.1144823351838113No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.11320320294712068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1770.11320320294712068No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT1680.10744710788201284No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.1055284095269769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.10041188058021439No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)