FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156356
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59663.8156514620481468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.3504822328532324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.34024917495970736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.28268822430862905No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.24687252168129142No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3800.2430351249712195No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.1976259305687022No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.19059070326690372No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.18355547596510527No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.18163677761006933No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.16948502136150834No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.16436849241474583No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.15669369899460206No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG2340.1496584716928036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.1496584716928036No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.14518150886438638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2230.14262324439100516No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.12983192202409885No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1960.12535495919568165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.12471539307733633No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1940.12407582695899103No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.12151756248560976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.12087799636726444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT1870.11959886413057381No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.1144823351838113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.11128450459208473No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.10936580623704878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.1055284095269769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTGTG350.00225309569.6924670
GACACTT200.003854649160.9011153
GACCTGT150.004132429659.85053321
GGCTTGT250.00529756756.19567566
GTTGTTT250.005522666555.60826565
CCTAATT250.006385107553.60700662
AACGCCA500.00924721748.78471470
TACCATC300.001298239839.7088938
TAGACGC450.001832517137.0095456
GGGAGGA452.3377503E-433.16501218
CTCCTCA400.004862358330.33479925
ACCCTTT450.00494830230.22400736
CTACACA400.00532046629.7816684
AAGCATA450.007486516627.76957528
GGCTACA557.673292E-427.0742452
GGGTTAA757.672465E-427.0671653
TTTGGTT550.00901294726.73007441
TATCCTC450.00923583926.60023521
TGCCGCG700.009426245526.48012765
ACGGGGT3050.025.41203916
Produced by FastQC (version 0.11.5)