FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827042
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC50590.27689565976042146No Hit
GACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACACCTGAATGCCAGTGCTCCAGGTCTGACAGTAATAGTCAG42290.23146703797723317No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41590.2276357084292534No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC41180.22539164397972242No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG39630.21690798569491015No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACACCTG38460.2105041920218583No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37860.20722019526644708No Hit
CTCCCAGCCTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT36700.20087113487265207No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35960.19682087220764494No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34000.18609314947330166No Hit
CATCAAGGGGGACGGGACCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG33260.1820428868082945No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32550.1781568239810579No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31650.1732308288479411No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31630.17312136228942737No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30010.16425457104981714No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29930.16381670481576233No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29090.15921910935818662No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC27510.15057125123560378No Hit
GGGTCTCTCTCCCAGCCTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC27080.14821772022755908No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26870.14706832136316517No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26830.14684938824613775No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA25510.1396245953842331No Hit
GACTATTACTGTCAGACCTGGAGCACTGGCATTCAGGTGTTCGGCGGGGGGACCAAGGTGACCGTCCTAGGTCAG25510.1396245953842331No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24760.13551959943996908No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24680.13508173320591427No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24430.13371340122449293No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24130.1320714028467873No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT23650.12944420544245835No Hit
GTAATAGTCAGCCTCATCCTCAAACTGGAGGCTGGAGATGGTGAGGTAGC22760.12457294358859841No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22550.12342354472420448No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22490.12309514504866335No Hit
GTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGG22310.12210994602204No Hit
CCTGCACTCTGAGCAGTGGGCACAGAAGTTACGCCATCGCATGGCATCAA22080.12085108059913237No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21890.11981114829325216No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGGTCCCGTCCCCCTTGAT21600.11822388319480341No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21460.11745761728520746No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21190.11597981874527243No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21140.11570615234898815No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20980.1148304198808785No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCCC20620.11286002182763176No Hit
CACCTGCACTCTGAGCAGTGGGCACAGAAGTTACGCCATCGCATGGCATC20180.11045175754033022No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20090.10995915802701854No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19810.10842662620782664No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19470.10656569471309363No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18970.10382903075025095No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG18820.10300803156139814No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18810.1029532982821413No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18730.10251543204808647No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC18490.10120183334592198No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18420.100818700391124No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTTTGAGGATGAGGCTGACTATTACTGTCAGAC18360.1004903007155829No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACAC18360.1004903007155829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGC200.00857174349.792158
TACGTAT1003.4528202E-642.04772670
GGTATCA24900.039.8811261
GTATCAA63050.036.820331
TATCAAC72300.032.0062982
CGTCTAA450.003900784331.75030550
GTGGTAT8650.031.484251
GGATGGG10250.031.4503370
ATCAACG74450.031.034863
TCAACGC75700.030.5215634
AACGCAG76750.030.3194376
TGGTATC9100.030.2815172
CAACGCA76450.030.2655545
TCCGAGA7550.029.70258570
CGCAGAG78600.029.4783028
ACGCAGA81000.028.604877
CCGTCAT2004.3788168E-728.03181670
AGAGTAC82100.028.01792111
GTACGTA1304.451631E-727.98118869
CAGAGTA82750.027.87806510
Produced by FastQC (version 0.11.5)