FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325684
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTATATCCCGTTTACCATACGGGGTTCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCA41660.3142528687077765No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40210.30331511883676654No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37340.28166591736793983No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33530.2529260366723895No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG31050.23421871275507586No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT28570.21551138883776225No Hit
ATATAGGATAGATCAGGAGTTTAGGGGCTTCTCCTGCTTTCTGCTGATACCAGCTTAAATTATTTCCAATGTC28220.21287124231717364No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26370.1989161821369195No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26320.19853901834826398No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26320.19853901834826398No Hit
GAAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGAAACA25980.1959743045854065No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23960.1807368875237236No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23940.18058602200826138No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23460.17696524963716845No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTGGAAA23340.17606005654439522No Hit
AGTCAGGACATTGGAAATAATTTAAGCTGGTATCAGCAGAAAGCAGGAGA23070.1740233720856554No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22430.1691956755908648No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22400.16896937731767148No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22370.16874307904447816No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21230.1601437446631324No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21140.15946484984355247No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21060.15886138778170364No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT20780.15674927056523275No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20470.15441085507556854No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20360.1535810947405264No Hit
ACCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGGACAAAGTGTTTCTG19720.1487533982457358No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT19480.1469430120601893No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19420.1464904155138027No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18850.14219074832312978No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18680.14090839144170103No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18540.1398523328334656No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18250.1376647828592636No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18170.13706132079741476No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17980.1356280984005238No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17930.1352509346118683No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17890.1349492035809439No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTCCACCTTGGTCCCTTGGCCG17890.1349492035809439No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17670.1332896829108596No Hit
GTATGTGGTATCACCAGTACCACTGATGACAGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATT17660.1332142501531285No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17440.13155472948304422No Hit
GTCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT17380.1311021329366576No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17320.130649536390271No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17250.13012150708615328No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16960.12793395711195127No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16880.12733049505010244No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16830.12695333126144692No Hit
GTCTACAAGATTACAGTTATCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGGTGGAAATCAAGCGAACTGTGG16520.12461491577178271No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16220.12235193303984963No Hit
GTATATTTCTGTGCGAAAGAGCATCTGTTTTCAGGGGGGTGGGGACTACC16210.12227650028211852No Hit
CTGTAATCTTGTAGACAGTAATAATTTCCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTG16080.12129587443161419No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15910.12001351755018541No Hit
CCTATATCCCGTTTACCATACGGGGTTCCATCAAGGTTCAGCGGCAGTGG15880.11978721927699211No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGGAAATTATTACTGTCTACAAGATTACAGTTAT15710.11850486239556335No Hit
AGATTACAGTTATCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGGTGG15700.11842942963783225No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15670.11820313136463893No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15480.11676990896774797No Hit
GTCTCTGTAGTCACCTTTAGCGGCTATGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGT15330.11563841760178141No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15250.11503495553993259No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15220.11480865726673929No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15060.11360173314304163No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT14990.1130737038389239No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTCCACCT14910.11247024177707507No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCGGG14810.11171591419976404No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14770.11141418316883962No Hit
TTCCTGTTCAGTCTCTGTAGTCACCTTTAGCGGCTATGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG14500.10937749871009984No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14330.1080951418286711No Hit
GGATATAGGATAGATCAGGAGTTTAGGGGCTTCTCCTGCTTTCTGCTGATACCAGCTTAAATTATTTCCAATGTC14310.1079442763132089No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT14110.10643562115858682No Hit
GATCAGGAGTTTAGGGGCTTCTCCTGCTTTCTGCTGATACCAGCTTAAAT14100.1063601884008557No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14100.1063601884008557No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14070.1061338901276624No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13990.10553042806581359No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGATGACCCAGTCTCCATCCTCC13850.10447436945757813No Hit
CTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGGACAAAGTGTTTCTGCAAATGGA13750.1037200418802671No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13440.10138162639060289No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATTCATGGCATAGCCGC13340.10062729881329185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)