FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325684
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTATATCCCGTTTACCATACGGGGTTCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCA38990.29411232239357193No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38590.2910950120843278No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31330.23633082997154675No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30470.22984361280667187No Hit
ATATAGGATAGATCAGGAGTTTAGGGGCTTCTCCTGCTTTCTGCTGATACCAGCTTAAATTATTTCCAATGTC28510.21505879229137562No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28050.21158888543574486No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27070.20419647517809675No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT26330.1986144511059951No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25180.1899396839669182No Hit
GAAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGAAACA24360.18375419783296773No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23990.18096318579691692No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22410.1690448100754026No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22380.16881851180220928No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22350.16859221352901596No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21610.16301018945691434No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21270.16044547569405682No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTGGAAA20670.1559195102301906No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20460.15433542231783742No Hit
AGTCAGGACATTGGAAATAATTTAAGCTGGTATCAGCAGAAAGCAGGAGA20370.1536565274982575No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20170.15214787234363544No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20100.1516198430395177No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20050.1512426792508622No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19930.15033748615808895No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19510.14716931033338262No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT19350.14596238620968496No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19010.14339767244682744No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18530.13977690007573448No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18450.13917343801388565No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18260.1377402156169947No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTCCACCTTGGTCCCTTGGCCG18260.1377402156169947No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18240.1375893501015325No Hit
ACCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGGACAAAGTGTTTCTG18170.13706132079741476No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17990.13570353115825493No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17950.1354018001273305No Hit
GTCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT17860.13472290530775055No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT17100.1289900157201867No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16660.12567097438001815No Hit
GTCTCTGTAGTCACCTTTAGCGGCTATGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGT16640.12552010886455595No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16610.12529381059136266No Hit
CTGTAATCTTGTAGACAGTAATAATTTCCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTG16600.12521837783363154No Hit
GTATGTGGTATCACCAGTACCACTGATGACAGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATT16530.12469034852951383No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16140.1217484709780008No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16000.12069241236976536No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15990.12061697961203424No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15910.12001351755018541No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15800.11918375721514328No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15580.11752423654505902No Hit
GTATATTTCTGTGCGAAAGAGCATCTGTTTTCAGGGGGGTGGGGACTACC15570.1174488037873279No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15510.11699620724094127No Hit
GTCTACAAGATTACAGTTATCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGGTGGAAATCAAGCGAACTGTGG15370.11594014863270583No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGGAAATTATTACTGTCTACAAGATTACAGTTAT15280.11526125381312591No Hit
AGATTACAGTTATCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGGTGG15250.11503495553993259No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15090.11382803141623493No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15050.11352630038531052No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14760.11133875041110854No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14750.11126331765337742No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT14620.11028269180287309No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14590.11005639352967976No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14530.10960379698329314No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14430.10884946940598213No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14390.10854773837505771No Hit
CCTATATCCCGTTTACCATACGGGGTTCCATCAAGGTTCAGCGGCAGTGG14310.1079442763132089No Hit
TTCCTGTTCAGTCTCTGTAGTCACCTTTAGCGGCTATGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG14240.10741624700909115No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14110.10643562115858682No Hit
GATCAGGAGTTTAGGGGCTTCTCCTGCTTTCTGCTGATACCAGCTTAAAT13790.10402177291119151No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13720.10349374360707378No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13680.10319201257614936No Hit
CTCCTGATCTATCCTATATCCCGTTTACCATACGGGGTTCCATCAAGGTT13630.10281484878749386No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13520.10198508845245172No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTCCACCT13440.10138162639060289No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC13360.10077816432875406No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCGGG13340.10062729881329185No Hit
CTTGTAGACAGTAATAATTTCCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATC13300.10032556778236744No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13270.10009926950917414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)