FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280371
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATAATGTCCCTCAAACATTCGGCGGAGGGACCAACGTGGAGATCAAACGAACTGTGGCTGCACCAT85260.66590074283157No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76290.5958429236525975No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC69370.5417960887898898No Hit
TTATCATACTGGTGACAGTAATATGTTGCAATGTCTTCAGGCTGCAGGCTGCTGATGGTGAAAATAAAATCTCTC65150.5088368918071403No Hit
CCTAATATATGATGCATCGAAATTGCAAACAGGGGTCCCGTCAAGATTCA56940.4447148521795636No Hit
ATATTACTGTCACCAGTATGATAATGTCCCTCAAACATTCGGCGGAGGGA56350.4401068127909801No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49890.3896526866041171No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG49490.38652859210338253No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48470.3785621511265094No Hit
CAGTAATATGTTGCAATGTCTTCAGGCTGCAGGCTGCTGATGGTGAAAATAAAATCTCTCCCAGATCCACTTCCA44000.3436503950808008No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43630.34076060766762134No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43350.3385737415171071No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42810.3343562139411155No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA42050.32842043438971985No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41560.32459341862632No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38280.2989758437202967No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG37580.29350867834401123No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36360.28398019011677084No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33950.26515752074984517No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33920.26492321366229005No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33140.2588312293858577No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32870.2567224655978618No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG32720.2555509301600864No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31170.24344506396974003No Hit
ACATATTACTGTCACCAGTATGATAATGTCCCTCAAACATTCGGCGGAGGGACCAACGTGGAGATCAAACGAA30420.23758738678086272No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCCCTCCGCCGAATGTT30410.23750928441834437No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAAAATAAAATCTCTCCCAGATCCACTTCCACTGAATCTTGACGGGAC30180.235712930080422No Hit
GCCTGAAGACATTGCAACATATTACTGTCACCAGTATGATAATGTCCCTC28420.22196691427718998No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27780.21696836307601466No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGACAT27330.21345375676268832No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC27060.21134499297469247No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26330.20564352051085194No Hit
GTAATATGTTGCAATGTCTTCAGGCTGCAGGCTGCTGATGGTGAAAATAAAATCTCTCCCAGATCCACTTCCA25710.2008011740347134No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24910.19455298503324428No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24610.19220991415769337No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23940.18697705586896296No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23510.18361865428067334No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23110.18049455977993878No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22810.17815148890438787No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT22730.17752667000424097No Hit
ATATATTAGGAGCCTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAACCACTTCTCAATGTCCCGACTCGC22030.1720595046279555No Hit
CCCCTAGGCTCCTAATATATGATGCATCGAAATTGCAAACAGGGGTCCCG21970.1715908904528453No Hit
ACCCAGTCTCCATCGTCCCTGTCTGCATTTGTGGGAGACAGAGTCACCAT21600.16870110303966585No Hit
ATTGAGAAGTGGTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAG21110.164874087276266No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT21090.1647178825512293No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20830.16268722112575185No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCC20670.16143758332545802No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20450.159719331350054No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20390.15925071717494382No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20370.1590945124499071No Hit
CCATCGTCCCTGTCTGCATTTGTGGGAGACAGAGTCACCATCACTTGCCA20350.1589383077248704No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20320.15870400063731527No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20230.15800107937465002No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20110.15706385102442963No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20080.15682954393687457No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGACATCCGGATGACCCAGTCTCCATCGTCC20000.15620472503672764No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19830.15487698487391544No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19750.15425216597376853No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19700.15386165416117673No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19330.15097186674799726No Hit
GCCTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAACCACTTCTCA19200.14995653603525852No Hit
GTCTCCATCGTCCCTGTCTGCATTTGTGGGAGACAGAGTCACCATCACTT19140.14948792186014834No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18900.14761346515970764No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18730.14628572499689543No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18550.14487988247156489No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18520.1446455753840098No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18320.14308352813364253No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18060.14105286670816505No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17980.14042804780801815No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGA17370.13566380369439796No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17160.1340236540815123No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16690.1303528430431492No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT16690.1303528430431492No Hit
ATATATGATGCATCGAAATTGCAAACAGGGGTCCCGTCAAGATTCAGTGG16680.13027474068063083No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16350.12769736271752485No Hit
AGTCGGGACATTGAGAAGTGGTTAAATTGGTATCAGCAAAAACCAGGGAA16330.1275411579924881No Hit
CTTCTCAATGTCCCGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCCACAAATGCAGACAGGGACGATGGAGA15930.12441706349175356No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15740.12293311860390466No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15740.12293311860390466No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15600.12183968552864756No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15590.1217615831661292No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT15570.12160537844109245No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15540.12137107135353738No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15430.12051194536583537No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15350.11988712646568846No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG15280.1193404099280599No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15040.11746595322761919No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14840.11590390597725192No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14810.11566959888969681No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGACATCCGGATGACCCAGTCT14270.11145207131370517No Hit
CACCATCACTTGCCAGGCGAGTCGGGACATTGAGAAGTGGTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCC14080.10996812642585627No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13970.10910900043815425No Hit
GAGTCACCATCACTTGCCAGGCGAGTCGGGACATTGAGAAGTGGTTAAATTGGTATCAGCAAAAACCAGGGAAAG13730.10723454373771353No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13610.10629731538749315No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13490.10536008703727279No Hit
GGCTCTCAGGTGGCAGATGTGACATCCGGATGACCCAGTCTCCATCGTCC13460.1051257799497177No Hit
GCCCCTAGGCTCCTAATATATGATGCATCGAAATTGCAAACAGGGGTCCCGTCAAGATTCAGTGGAAGTGGAT13270.10364183506186879No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12930.10098635473624441No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12820.10012722874854242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)