FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280371
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATAATGTCCCTCAAACATTCGGCGGAGGGACCAACGTGGAGATCAAACGAACTGTGGCTGCACCAT79780.6231006481715066No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT77690.6067772544051685No Hit
TTATCATACTGGTGACAGTAATATGTTGCAATGTCTTCAGGCTGCAGGCTGCTGATGGTGAAAATAAAATCTCTC63970.4996208130299733No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55870.43635789939009867No Hit
ATATTACTGTCACCAGTATGATAATGTCCCTCAAACATTCGGCGGAGGGA53690.4193315843610953No Hit
CCTAATATATGATGCATCGAAATTGCAAACAGGGGTCCCGTCAAGATTCA52080.4067571039956388No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50890.39746292285595347No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50800.39676000159328817No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG46580.36380080461053865No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42870.3348248281162257No Hit
CAGTAATATGTTGCAATGTCTTCAGGCTGCAGGCTGCTGATGGTGAAAATAAAATCTCTCCCAGATCCACTTCCA42390.3310759147153442No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40950.3198291745126999No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40730.31811092253729584No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39800.310847402823088No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39720.3102225839229411No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT35940.28069989089099956No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34660.270702788488649No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34340.2682035128880613No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33290.26000276482363316No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33260.25976845773607804No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32520.2539888829097191No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32510.2539107805472008No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32460.253520268734609No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31230.2439136781448502No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAAAATAAAATCTCTCCCAGATCCACTTCCACTGAATCTTGACGGGAC30970.24188301671937273No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG29820.23290124502976092No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCCCTCCGCCGAATGTT29800.23274504030472418No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29170.22782459146606726No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT29030.22673115839081018No Hit
GCCTGAAGACATTGCAACATATTACTGTCACCAGTATGATAATGTCCCTC27650.21595303236327598No Hit
ACATATTACTGTCACCAGTATGATAATGTCCCTCAAACATTCGGCGGAGGGACCAACGTGGAGATCAAACGAA27470.2145471898379454No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC25240.19713036299635026No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24370.1903354574572526No Hit
GTAATATGTTGCAATGTCTTCAGGCTGCAGGCTGCTGATGGTGAAAATAAAATCTCTCCCAGATCCACTTCCA24060.18791428421918335No Hit
ATATATTAGGAGCCTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAACCACTTCTCAATGTCCCGACTCGC23650.18471208735593045No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23650.18471208735593045No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23430.18299383538052644No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23390.18268142593045297No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22970.17940112670468167No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22790.17799528417935115No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGACAT22380.17479308731609822No Hit
CCCCTAGGCTCCTAATATATGATGCATCGAAATTGCAAACAGGGGTCCCG22380.17479308731609822No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21290.16627992980159656No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21030.1642492683761191No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT20770.16221860695064166No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20730.16190619750056817No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT20510.1601879455251642No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20160.15745436283702147No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20030.15643903212428273No Hit
ACCCAGTCTCCATCGTCCCTGTCTGCATTTGTGGGAGACAGAGTCACCAT19940.15573611086161745No Hit
ATTGAGAAGTGGTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAG19890.15534559904902562No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19390.15144048092310744No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19330.15097186674799726No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19280.15058135493540545No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19280.15058135493540545No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19180.1498003313102218No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGACATCCGGATGACCCAGTCTCCATCGTCC19170.14972222894770346No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18950.14800397697229944No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18670.14581711082178525No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18280.14277111868356904No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17910.1398813312703896No Hit
GTCTCCATCGTCCCTGTCTGCATTTGTGGGAGACAGAGTCACCATCACTT17820.13917841000772432No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17700.13824118165750396No Hit
GCCTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAACCACTTCTCA17440.13621052023202648No Hit
CCATCGTCCCTGTCTGCATTTGTGGGAGACAGAGTCACCATCACTTGCCA17380.1357419060569163No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGA17160.1340236540815123No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17040.13308642573129195No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16980.13261781155618177No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16880.13183678793099812No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16810.13129007139336957No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16550.12925940996789212No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16090.12566670129204738No Hit
AGTCGGGACATTGAGAAGTGGTTAAATTGGTATCAGCAAAAACCAGGGAA16040.12527618947945557No Hit
CTTCTCAATGTCCCGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCCACAAATGCAGACAGGGACGATGGAGA16010.12504188239190048No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15700.12262070915383119No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT15630.12207399261620264No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCGGGACATTGAGAAGTGGTTAAATTGGTATCAGCAAAAA15600.12183968552864756No Hit
ATATATGATGCATCGAAATTGCAAACAGGGGTCCCGTCAAGATTCAGTGG15450.1206681500908721No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCC15260.1191842052030232No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15240.11902800047798646No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15220.11887179575294973No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15120.1180907721277661No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15100.11793456740272937No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15080.11777836267769264No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15000.11715354377754572No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14990.11707544141502738No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14880.11621631542732536No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14480.11309222092659081No Hit
GAGTCACCATCACTTGCCAGGCGAGTCGGGACATTGAGAAGTGGTTAAATTGGTATCAGCAAAAACCAGGGAAAG14440.11277981147651735No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGGCAGATGTGACATCCGGATGACCCAGTCT14320.11184258312629698No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14120.11028053587592972No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT13730.10723454373771353No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13420.10481337049964425No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG13220.10325132324927697No Hit
TATATTAGGAGCCTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAACCACTTCTCAATGTCCCGACTCGC13070.10207978781150151No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13020.1016892759989097No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG12990.1014549689113546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2950.039.1438181
GTATCAA31950.038.4807851
TATCAAC34000.035.845532
ATCAACG35300.034.7069363
AACCGCA400.002531899134.67749842
TCAACGC35650.034.178474
TTAGTAC400.002814250533.9378053
CAACGCA35800.033.844335
AACGCAG36500.033.2882466
CACGACT500.00420172731.27091268
ACGCAGA39150.031.0338047
ATTAGTA553.5800168E-430.8621962
CGCAGAG39650.030.6436548
AGAGTAC40300.030.23361811
GCGCACT450.005008728730.16811811
CAGAGTA40550.029.88099510
ATAGGAC803.139232E-629.695583
GCAGAGT41050.029.5985589
GCGCGTG4850.027.66961770
CCGGTCG808.632865E-526.26711549
Produced by FastQC (version 0.11.5)