FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519846
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTATCTTGGTTCCAGCAAAA169741.116823678188448No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTATCCGGGAATCCGGGGTCCCT117790.7750127315530653No Hit
GTATAAAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCTCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA106580.7012552587564793No Hit
GTTTAGGAGGCTGTCCTGGTTTTTGCTGGAACCAAGATAAGTAGTTCTTATTGTTGGAGCTGTATAAAACAGT104260.68599055430616No Hit
ATATTATAGTACTCCCTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT100520.6613827979940072No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT91240.60032398019273No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC90250.5938101623453955No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTC75750.49840575953089983No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC65470.430767327742416No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC65400.4303067547633116No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG61080.401882822338579No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA59230.3897105364622468No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG56650.3727351323752538No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC53590.3526015135744016No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT52450.34510075362898607No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG49140.32332223133133225No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG47570.3129922373714179No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG47200.31055778019615143No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT45850.3016753013134226No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCCTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA45020.29621422170404105No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT44110.29022677297568306No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT43220.2843709165270692No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC43090.2835155667087323No Hit
GTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCA41820.27515945694497995No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCAC40920.269237804356494No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39890.2624608019496712No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38120.25081488519231554No Hit
TTATAGTACTCCCTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT37710.24811724345756084No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTG37120.24423527120510896No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC37090.24403788278549274No Hit
GTGTAGGGAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTCC34320.22581235204093045No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGAGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATA34200.22502279836246566No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAAT33950.22337789486566403No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG33740.2219961759283506No Hit
GTCAGGGACCCCGGATTCCCGGATAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTTTGCTGGAA32810.2158771349202485No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGAGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACAGCTCCAA32700.2151533773816558No Hit
ATAGTACTCCCTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGA32590.21442961984306305No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA32450.2135084738848541No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA32410.21324528932536585No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32320.21265312406651726No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG32140.2114687935488201No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA31360.20633669463879895No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCACCATCAGCAGCC30410.20008606135095267No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGAGCCACCATCAACTGCAAGTCCAGC28810.1895586789714221No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTATCCGGGAA28690.1887691252929573No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT28580.1880453677543646No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28570.18797957161449252No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT28420.18699262951641152No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA28140.1851503375999937No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC27950.18390021094242442No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA27790.18284747270447138No Hit
GCTCATTTACTGGGCATCTATCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT27680.18212371516587866No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27190.1788997043121474No Hit
TCTCCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC26750.17600467415777651No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC26560.17475454750020725No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT26380.17357021698251007No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT26320.17317544014327768No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT26210.17245168260468496No Hit
CCCTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG26140.1719911096255805No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG25570.16824072965287273No Hit
GTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTTTGCTGGAACCAAGATA25410.16718799141491966No Hit
CAGTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCA25360.16685901071555934No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT25330.16666162229594314No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCC25320.16659582615607108No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC25020.1646219419599091No Hit
GGCCAAAAGTGTAGGGAGTACTATAATATTGCTGACAGTAATAAACTGCC24850.16350340758208398No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA24830.16337181530233982No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC24700.16251646548400298No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT24250.15955563918976No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC24110.1586344932315511No Hit
GATTTCTCTCTCACCATCAGCAGCCTGCAGGCTGGAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT24100.15856869709167903No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA23560.15501570553858746No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGAGCCACCATCAACTG23480.15448933641961093No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC23370.15376557888101822No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG23120.15212067538421656No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAAT22830.15021258732792664No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22790.14994940276843838No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22760.14975201434882218No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG22680.14922564522984566No Hit
GGCTGGAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCCT22670.14915984908997357No Hit
CTACTTATCTTGGTTCCAGCAAAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTATCCGGGAATC22650.14902825681022944No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT21710.14284341966225525No Hit
ATCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCACCATCAGCAGC21610.1421854582635346No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC21390.14073794318634913No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT21220.13961940880852403No Hit
GTCCTGGTTTTTGCTGGAACCAAGATAAGTAGTTCTTATTGTTGGAGCTG21100.13882985513005922No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20610.135605844276328No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG20580.1354084558567118No Hit
GTCTCTGGGCGAGAGAGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACAGCTCCAACAATAAGAACTA20330.13376355235991017No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGAG20300.13356616394029397No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20300.13356616394029397No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20180.13277661026182916No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGAT19860.13067113378592304No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19850.13060533764605098No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC19630.12915782256886554No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19560.12869724958976106No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG19480.12817088047078454No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC19440.12790769591129628No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA18880.12422311207846057No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18820.12382833523922818No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC18800.12369674295948405No Hit
TTATTACTGTCAGCAATATTATAGTACTCCCTACACTTTTGGCCAGGGGA18760.12343355839999579No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA18710.12310457770063545No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT18660.12277559700127512No Hit
GTCTGGGACAGATTTCTCTCTCACCATCAGCAGCCTGCAGGCTGGAGATG18630.12257820858165892No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18560.12211763560255448No Hit
ACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACAGCTCCAACAATAA18050.11876203246907911No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC18010.11849884790959084No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGATAGATGCCCAGTAAAT17970.11823566335010258No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17870.11757770195138192No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17750.11678814827291713No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCACCATCAG17600.11580120617483614No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGATAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTTTG17600.11580120617483614No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA16970.11165604936289597No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT16820.11066910726481498No Hit
CAATAAGAACTACTTATCTTGGTTCCAGCAAAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTAT16770.11034012656545465No Hit
GATAAGTAGTTCTTATTGTTGGAGCTGTATAAAACAGTCTGGCTGGACTT16430.10810305780980442No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG16380.1077740771104441No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16340.10751089255095583No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT16330.10744509641108377No Hit
GCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTGTAGGG16020.10540541607504972No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGC15880.10448427011684079No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT15620.10277357048016707No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG15320.10079968628400508No Hit
CTCCCTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15290.10060229786438889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)