FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519846
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTATCTTGGTTCCAGCAAAA149810.9856919714234205No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTATCCGGGAATCCGGGGTCCCT106890.7032949390925134No Hit
ATATTATAGTACTCCCTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT98160.6458549089841997No Hit
GTTTAGGAGGCTGTCCTGGTTTTTGCTGGAACCAAGATAAGTAGTTCTTATTGTTGGAGCTGTATAAAACAGT97910.644210005487398No Hit
GTATAAAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCTCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA91540.6022978643888921No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT89930.5917046858694894No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTC77490.5098542878686393No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC73600.4842595894584057No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC68120.44820330480851345No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC63380.4170159345091542No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG54080.35582552442813287No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT53990.3552333591692843No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA53350.35102240621747205No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC48300.3177953555820787No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT48100.3164794327846374No Hit
GTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCA46070.303122816390608No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC45530.29956982483751643No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG44920.29555626030532045No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCCTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA44420.29226645331171713No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG43460.28595002388399876No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT43370.2853578586251502No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG42510.2796993905961525No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42340.2785808562183274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41210.2711458924127839No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT40510.2665401626217393No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39630.2607501023129975No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCAC39000.25660494550105734No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC38610.2540388960460468No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTG37190.2446958441842134No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAAT36670.24127444491086597No Hit
TTATAGTACTCCCTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT36610.24087966807163358No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA35530.23377368496545042No Hit
GTGTAGGGAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTCC35460.233313111986346No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGAT34530.22719407097824387No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG33020.2172588538575619No Hit
TCTCCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC32880.21633770789935297No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32700.2151533773816558No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA32170.21166618196843626No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG31400.2065998791982872No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGAGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATA30910.20337586834455598No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGAGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACAGCTCCAA29880.19659886593773315No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA29800.19607249681875663No Hit
GTCAGGGACCCCGGATTCCCGGATAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTTTGCTGGAA29620.19488816630105946No Hit
ATAGTACTCCCTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGA29500.19409861262259465No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29120.19159835930745614No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28760.18922969827206176No Hit
CAGTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCA28140.1851503375999937No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28080.1847555607607613No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCACCATCAGCAGCC27670.1820579190260066No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGAGCCACCATCAACTGCAAGTCCAGC27540.18120256920766972No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA27510.18100518078805353No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT26980.17751798537483401No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26800.17633365485713684No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26670.17547830503879996No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA26570.1748203436400793No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC25960.1708067791078833No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC25830.16995142928954646No Hit
GGCCAAAAGTGTAGGGAGTACTATAATATTGCTGACAGTAATAAACTGCC25770.16955665245031404No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC25750.1694250601705699No Hit
CCCTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG25150.16547729177824594No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24710.16258226162387504No Hit
GCTCATTTACTGGGCATCTATCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT24600.16185850408528232No Hit
GGCTGGAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCCT24580.1617269118055382No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT24570.16166111566566613No Hit
GATTTCTCTCTCACCATCAGCAGCCTGCAGGCTGGAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT24400.16054258128784102No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC24380.1604109890080969No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTCCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAAT24180.15909506621065556No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTATCCGGGAA23860.15698958973474944No Hit
GTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTTTGCTGGAACCAAGATA23680.15580525921705227No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23270.15310761748229754No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22960.1510679371462635No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT22860.15040997574754283No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA22830.15021258732792664No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22700.1493572375095898No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT21620.14225125440340666No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA21610.1421854582635346No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21570.14192227370404634No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA21490.14139590458506981No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21470.14126431230532566No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC21210.13955361266865196No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGAGCCACCATCAACTG21130.1390272435496754No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21030.13836928215095476No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC20940.13777711689210617No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCC20450.13455310603837495No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG20300.13356616394029397No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20290.1335003678004219No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT20190.13284240640170122No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20030.13178966816374818No Hit
GTCCTGGTTTTTGCTGGAACCAAGATAAGTAGTTCTTATTGTTGGAGCTG19710.12968419168784207No Hit
CTACTTATCTTGGTTCCAGCAAAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTATCCGGGAATC19700.12961839554797No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGAG19610.12902623028912139No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19580.1288288418695052No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA19460.1280392881910404No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19130.12586801557526223No Hit
GTCTGGGACAGATTTCTCTCTCACCATCAGCAGCCTGCAGGCTGGAGATG19100.12567062715564603No Hit
ATCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCACCATCAGCAGC18980.12488107347718123No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18810.12376253909935611No Hit
GTCTCTGGGCGAGAGAGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACAGCTCCAACAATAAGAACTA18680.12290718928101925No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18540.12198604332281035No Hit
GAGAGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG18500.12172285876332209No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG18500.12172285876332209No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG18230.1199463629867763No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC17940.11803827493048638No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17790.11705133283240539No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT17770.11691974055266126No Hit
TTATTACTGTCAGCAATATTATAGTACTCCCTACACTTTTGGCCAGGGGA17490.1150774486362434No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG16880.11106388410404737No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC16860.11093229182430324No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16620.10935318446737367No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTGTAGGGAGTA16510.10862942692878096No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT16480.10843203850916475No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA16440.10816885394967649No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC16430.10810305780980442No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGC16140.1061949697535145No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT16140.1061949697535145No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGG15960.10501063923581731No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCACCATCAG15860.10435267783709666No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG15810.10402369713773632No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGATAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTTTG15800.10395790099786426No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15760.103694716438376No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGATAGATGCCCAGTAAAT15720.10343153187888773No Hit
CTCCCTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15540.10224720136119055No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA15430.10152344382259781No Hit
GCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTGTAGGG15340.10093127856374921No Hit
ACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACAGCTCCAACAATAA15230.1002075210251565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)