FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100291
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGTTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT105130.9554745062896997No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAACTCTGTC94310.8571368846968667No Hit
CTTTTATACAGCTCCAATAGTAAGAACTACTTAGCTTGGTACCAGCAGAA70100.6371041842567103No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67320.6118381410008806No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50400.45806064032151494No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46640.423887862392767No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44300.4026207612349824No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38450.3494530083405208No Hit
GAGTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTGTT38150.3467264569100356No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGAAGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA37850.3439999054795504No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36620.3328210446145611No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTATTGGAGCTGTATAAAAGACT35500.3226419192740829No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT34490.3134625294581161No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34420.3128263341243362No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT32370.29419489934935394No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31810.28910533667911487No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30340.2757452346697374No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30020.27283691314388653No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29830.2711100972379125No Hit
GAGTGAACTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG29810.27092832714254683No Hit
GTTTATTACTGTCAGCAATATTATAGTGTTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA28930.2629304429464569No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAACTCTGTCCCAGACCCGCTGCCA28530.25929504103914325No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28510.2591132709437776No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28260.2568411447517066No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28080.25520521389341544No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27200.24720732969732553No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26880.24429900817147465No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26850.24402635302842612No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26670.24239042217013498No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26030.23657377911843322No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG25830.23475607816477642No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25680.23339280244953378No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24650.22403164253820126No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAGTTCACTCTCAC23350.21221658633943202No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCAGCCAGAGTCTTTTATA23150.2103988853857752No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA21950.1994926796638344No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21170.19240364594457285No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATG20600.18722319822665096No Hit
GCTCCAATAGTAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGG20580.18704142813128527No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19930.18113390003190064No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19770.1796797392689752No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19560.17777115326763557No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19130.17386309621727344No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCA18560.16868264849935155No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18090.16441105125825803No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17960.1632295456383811No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17800.16177538487545567No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17700.16086653439862728No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17500.15904883344497045No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17390.15804909792045924No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA17140.15577697172838822No Hit
GTTGATGGTGAGAGTGAACTCTGTCCCAGACCCGCTGCCACTGAATCGGT17000.15450458106082846No Hit
ATTATAGTGTTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA16750.15223245486875744No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16650.15132360439192905No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTATTGGAGCTG16550.15041475391510065No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16550.15041475391510065No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAACTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC16520.15014209877205212No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGAGTTCACTCTCACCATCAACAGCC16460.1495967884859551No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTGTTCCCCTCACTTT16270.1478699725799811No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16040.14577961648327578No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15960.14505253610181307No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15870.1442345706726675No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15860.14414368562498467No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15780.14341660524352193No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15660.14232598467132784No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15270.1387814678116971No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCAGC15100.1372364220010888No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAACTCTGTCCCAGACCCGCTGCCA15010.13641845657194326No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14980.13614580142889474No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14950.1358731462858462No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14920.13560049114279768No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG14890.13532783599974915No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14690.13351013504609235No Hit
GTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCAGCCAGAGTCTTTTATACAGCTCCAATAGTAAGAACTA14430.1311471238063385No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGC14270.12969296304341305No Hit
GGATCTCTGGTGCCTGCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14140.12851145742353615No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTGTTCCCC14100.12814791723280478No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14050.12769349199439056No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13950.12678464151756216No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13670.12423986018244265No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATC13670.12423986018244265No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13590.12351277980097992No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13580.12342189475329707No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13440.12214950408573733No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13410.1218768489426888No Hit
CACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAACT13300.12087711341817754No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13240.12033180313208051No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13200.11996826294134914No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13170.11969560779830063No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13100.11905941246452074No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13080.11887764236915507No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12920.11742348160622962No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12890.11715082646318109No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12750.11587843579562135No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12700.11542401055720715No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12590.1144242750326959No Hit
ACCTTCAACTGCAAGTCCAGCCAGAGTCTTTTATACAGCTCCAATAGTAA12530.11387896474659885No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTGCGGGGACATCGTGATGA12430.11297011426977044No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12360.11233391893599057No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12120.1101526777916024No Hit
CTATTGGAGCTGTATAAAAGACTCTGGCTGGACTTGCAGTTGAAGGTGGC11970.10878940207635979No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC11940.10851674693331127No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11520.10469957493063199No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11500.10451780483526632No Hit
CCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAA11300.1027001038816095No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11270.102427448738561No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT11190.10170036835709827No Hit
ATAGTGTTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA11050.10042797768953848No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG11050.10042797768953848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)