FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121358
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65810.5868776965072706No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62450.5569140274559953No Hit
ATATTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT58600.5225806566680756No Hit
CTTTTATACAACTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA50970.45453815819747123No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44570.39746450286170876No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44220.3943432873355342No Hit
TTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT40010.35679952343497795No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38910.3469899889241438No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38840.34636574581890883No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37760.336734566480999No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37560.33495101475175637No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36920.3292436492181801No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34220.3051657008734053No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33860.3019553077607686No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32060.28590334219758545No Hit
CTCCTAAATTGCTCATTTACTGGGCTTCTACCCGGGACTCCGGGGTCCCT30550.27243752664180393No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30130.2686920680103946No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA29850.2661950955894549No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29750.2653033197248337No Hit
ATTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTGTATAAAAGACT29620.26414401110082597No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29470.262806347303894No Hit
AACCAGGACAGCCTCCTAAATTGCTCATTTACTGGGCTTCTACCCGGGAC29120.2596851317777195No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28630.2553154300410752No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTCGCTCTGGATCTCTGGTGC28030.24996477485334745No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27140.242027969658218No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC27130.24193879207175584No Hit
GTCCCTGACCGATTCAGTGGCAGCGGCTCTGGGACAGATTTCACTCTCAC26650.23765826792157366No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24890.221963012704239No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCGCGCCCAGAGACACAGGCAGGGAGTCTGGAGA24320.2168798902758976No Hit
CTCCCTGCCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACAACTCCAA23170.2066244678327528No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT23020.20528680403582084No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22570.20127381264502506No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22540.20100627988563868No Hit
ACCCGGGACTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGCTCTGGGACAGATTTCACTCTCACCATCAGCAGC22210.1980634195323884No Hit
GTCTCCAGACTCCCTGCCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA22070.1968149333219186No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT21940.19565562469791095No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21700.19351536262281982No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21610.19271276434466067No Hit
GTCAGGGACCCCGGAGTCCCGGGTAGAAGCCCAGTAAATGAGCAATTTAGGAGGCTGTCCTGGTTTCTGCTGGTA20630.18397336087137203No Hit
ATTCAGTGGCAGCGGCTCTGGGACAGATTTCACTCTCACCATCAGCAGCC20570.18343829535259926No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20450.1823681643150537No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20430.18218980914212946No Hit
GTGCTGACCCAGTCTCCAGACTCCCTGCCTGTGTCTCTGGGCGCGAGGGC19720.1758582005033183No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19320.17229109704483314No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTG19290.17202356428544674No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18870.16827810565403734No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18710.16685126427064328No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18040.16087636597768062No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18030.1607871883912185No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17960.1601629452859836No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17630.15722008493273334No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17620.15713090734627122No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17530.15632830906811204No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17370.154901467684718No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17170.15311791595547541No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17100.1524936728502405No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16850.15026423318868729No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16850.15026423318868729No Hit
GTGTATTACTGTGCGAGAGATATCCAAGATTACTATGTCAGTAGTGCTAA16840.15017505560222516No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16820.14999670042930088No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16650.1484806814594447No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16580.1478564383542098No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16250.14491357800095955No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAATTGCTCATTTACTGGGCTTCTACCCGGGACTC16220.14464604524157318No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16220.14464604524157318No Hit
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTCCATCTGCAGATACA16090.1434867366175655No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16080.14339755903110338No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCTCGGACGTT15790.14081140902370162No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15640.1394737452267697No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTC15450.13777937108398924No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15200.13554993142243602No Hit
ATAGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA15200.13554993142243602No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15120.134836510730739No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAATTGCTCATTTACTGGGCTTCTACCCGGGA15100.1346581555578147No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAATTGCTCATT15030.1340339124525798No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14960.1334096693473449No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14900.13287460382857214No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG14890.13278542624211No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGAGCCGCTGCCA14630.13046680899409466No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14470.12903996761070058No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14300.1275239486408444No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14270.127256415881458No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14030.12511615380636693No Hit
GTCCCGGGTAGAAGCCCAGTAAATGAGCAATTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTT13930.12422437794174565No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13910.12404602276882139No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13830.12333260207712435No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13830.12333260207712435No Hit
ATTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA13810.1231542469042001No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13570.121013984829109No Hit
GGATCTCTGGTGCCTGCGGGGACATCGTGCTGACCCAGTCTCCAGACTCCCTGCCTGTGTCTCTGGGCGCGAGGG13540.1207464520697226No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13370.11923043309986642No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13270.11833865723524513No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCTC13240.11807112447585874No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13150.11726852619769958No Hit
GGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACAACTCCAACA13120.11700099343831319No Hit
CTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC12940.11539579688199487No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12910.11512826412260849No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12910.11512826412260849No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTCGCTCTGGATCTCTGGTGCCTGCGGGGACATCGTGCTGA12900.11503908653614635No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12860.11468237619029784No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC12840.11450402101737357No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA12640.112720469288131No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12620.11254211411520675No Hit
CTCCAGACTCCCTGCCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTGC12550.11191787100997183No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12520.11165033825058544No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12500.1114719830776612No Hit
GTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACAACTCCAACAATAAGAACTA12180.10861830031087306No Hit
CCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG12050.10745899168686539No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTCGCTCTGGATCTCTGGTGCCTGCGGGGACATCGTG12040.10736981410040325No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTCGCTCTGGAT11990.10692392616809261No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA11870.10585379513054706No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11780.1050511968523879No Hit
GCAGAAACCAGGACAGCCTCCTAAATTGCTCATTTACTGGGCTTCTACCCGGGACTCCGGGGTCCCTGACCGATT11730.10460530892007726No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11560.10308928995022106No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11460.10219751408559977No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACAACTCCAACAATAA11400.10166244856682702No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAATTGCTCATTTACTGGGCTTCTACCCGGGACTCCGGGGTCCCTGA11390.1015732709803649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)