FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004692177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206366
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT74063.588769467838694No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC11240.5446633650892104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.37796923911884706No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7130.3455026506304333No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.30867487861372517No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG6310.30576742292819553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.26457846738319296No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.22048205615266078No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4490.2175746004671312No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.2161208726243664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.21563629667677814No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.20449104988224803No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.17880852466006997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3660.17735479681730518No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.17735479681730518No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.171539885446246No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT3510.1700861576034812RNA PCR Primer, Index 47 (95% over 22bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.16960158165589292No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.1686324297607164No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.1657249740751868No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.1599100627041276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.14682651211924447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.14585736022406792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.13858872101024391No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.13858872101024391No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2720.1318046577440082No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.13132008179641994No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.1279280501633021No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.12259771473983117No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATGTCGTATGCCGT2510.12162856284465465No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2500.12114398689706637No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2440.1182365312115368No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.11484449957841893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.11484449957841893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.11435992363083067No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAG2270.10999874010253627No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.10902958820735974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.10902958820735974No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT2200.10660670846941842No Hit
CTCCAGCCCCCACGTTGCACCAGACCCGTACTCTGCGTTGATAC2180.10563755657424188No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2130.10321467683630055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.10321467683630055No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.10224552494112402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)