FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206366
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116915.665177403254412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.38136127075196496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.374092631538141No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6710.32515046083172616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.26893965091148736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5220.2529486446410746No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.21418256883401335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.20546020177742455No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.20303732203948324No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.19043834740218835No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.18220055629318782No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.17444734113177557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3470.16814785381312813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.16766327786553986No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.1671787019179516No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3120.15118769564753884No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.15021854375236232No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3020.14634193617165617No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.14585736022406792No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG2980.1444036323813031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.13713499316747912No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT2770.13422753748194954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.1308355058488317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.12550517042536077No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.12550517042536077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.12453601853018423No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.12308229068741944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.11048331605012453No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2240.10854501225977146No Hit
CTCCAGCCCCCACGTTGCACCAGACCCGTACTCTGCGTTGATAC2240.10854501225977146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.10660670846941842No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.10273010088871228No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAG2110.10224552494112402No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2090.10127637304594748No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.10030722115077095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAAT200.00274278966.3686656
TTACAGC250.0043588759.04120364
AGAGACT200.00442341558.82977743
CTAAAAG150.004942991357.20991516
CACTAAA150.00498592657.08509414
CTGCTAT150.004990713757.071261
ACAGTAC150.004995504457.05742611
TGCTATA150.004995504457.0574262
ATGCTCG450.005920965754.62212870
GCCACAT250.006072684754.29884358
GAGTTGT250.00791260650.7856251
AATATCA300.00857224949.76246665
GTATAGA350.001057313841.40237439
ACTTGTA350.001546106538.3246135
CGCCACA500.0021746635.7458857
CTTCCCA401.5258197E-435.6695371
TATTAAA1151.5275968E-435.62312770
TACTTGT400.003270913632.896934
AGAGATC400.003270913632.896934
CTTCACT350.003418480332.60424411
Produced by FastQC (version 0.11.5)