FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436396
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57030.3970353579375047No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT54470.37921297469500054No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54260.3777509823196389No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42420.29532245982305716No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41570.2894048716370694No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39600.2756899907824862No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39350.2739495236689604No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36390.25334239304481493No Hit
AAGTAAAGATTGTCCCAGATCCACTCCCAATGAACCTTGATGGGACCCCT35570.24763366091245034No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG33400.23252640636704638No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33320.23196945689071816No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33040.23002013372356925No Hit
CTTTTGGCCAGGGGACCACGCTGGACATCAAACGAACTGTGGCTGCACCA32720.22779233581825625No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31240.2174887705061835No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31010.2158875407617398No Hit
ATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCACAACCATTTAAATTGGTATCAGCAGAAA30930.21533059128541154No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCACAA30900.21512173523178846No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30510.2124066065346882No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29900.20815986677768525No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29590.20600168755691328No Hit
GGCCAAAAGTGTACACAAATCCGTCCGTGTAGGGAGGGAGATTATCATAATGATGACAGTGATATGTAGCGATAT28900.20119799832358207No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28620.19924867515643319No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCGTGGTCCCCTGGCCAAAAGTG28470.1982043948883177No Hit
AGTCAGGACATTCACAACCATTTAAATTGGTATCAGCAGAAACCAGGGAA28380.19757782672744842No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGCGTGGTCC27780.1934007056549865No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27520.1915906198569197No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27210.1894324406361477No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26960.1876919735226219No Hit
CCCTACACGGACGGATTTGTGTACACTTTTGGCCAGGGGACCACGCTGGA26850.18692616799267056No Hit
ATCTTTACTTTGACCATCAGCAGCCTGCAGCCTGAAGATATCGCTACATATCACTGTCATCATTATGATAATCTC26270.1828882842892907No Hit
ACATATCACTGTCATCATTATGATAATCTCCCTCCCTACACGGACGGATT26250.18274904692020863No Hit
GTGATATGTAGCGATATCTTCAGGCTGCAGGCTGCTGATGGTCAAAGTAA25890.18024277427673147No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25640.1785023071632057No Hit
GTGTAGGGAGGGAGATTATCATAATGATGACAGTGATATGTAGCGATATC25580.1780845950559595No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC23970.16687598684485336No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATGGTTGTGA23560.16402162077867108No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22870.15921793154533986No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22480.1565028028482396No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22230.15476233573471385No Hit
CTCCTAATCTACGATGCGTCCGATTTGCAAACAGGGGTCCCATCAAGGTTCATTGGGAGTGGATCTGGGACAAT22210.15462309836563176No Hit
GTCCCAGATCCACTCCCAATGAACCTTGATGGGACCCCTGTTTGCAAATC22030.1533699620438932No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT21980.15302186862118802No Hit
GATATGTAGCGATATCTTCAGGCTGCAGGCTGCTGATGGTCAAAGTAAAG21230.14780046728061064No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20700.14411067699993596No Hit
GATTAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT20680.14397143963085388No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20630.14362334620814873No Hit
GATTTGTGTACACTTTTGGCCAGGGGACCACGCTGGACATCAAACGAACT19840.1381234701294072No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19700.13714880854583275No Hit
CCCCTAAACTCCTAATCTACGATGCGTCCGATTTGCAAACAGGGGTCCCA19230.13387673037240427No Hit
ATTCACAACCATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA19200.13366787431878116No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18850.13123122035984505No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18820.13102236430622197No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18820.13102236430622197No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT18790.13081350825259885No Hit
CTACATATCACTGTCATCATTATGATAATCTCCCTCCCTACACGGACGGATTTGTGTACACTTTTGGCCAGGGGA18790.13081350825259885No Hit
CAGTGATATGTAGCGATATCTTCAGGCTGCAGGCTGCTGATGGTCAAAGT18770.1306742708835168No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18660.12990846535356546No Hit
GTCCAGCGTGGTCCCCTGGCCAAAAGTGTACACAAATCCGTCCGTGTAGG18530.12900342245453203No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18490.12872494771636792No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18340.12768066744825243No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18320.12754143007917038No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18120.12614905638834972No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17900.12461744532844704No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17630.12273774084583916No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17440.12141498583955956No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17430.12134536715501852No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC17340.12071879899414925No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17330.12064918030960821No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17280.12030108688690305No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17280.12030108688690305No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17240.12002261214873894No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17210.11981375609511583No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17010.11842138240429519No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16690.11619358449898218No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCAC16600.11556701633811288No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACT16600.11556701633811288No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT16570.11535816028448981No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16220.11292150632555367No Hit
ACGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT16180.11264303158738954No Hit
GCCTGAAGATATCGCTACATATCACTGTCATCATTATGATAATCTCCCTCCCTACACGGACGGATTTGTGTACA16160.11250379421830747No Hit
GTCTCCTATAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG16150.11243417553376646No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTACGATGCGTCCGATTTGCAAACAGGGGTCCCATCAAGGTT16100.11208608211106129No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16080.11194684474197925No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC16010.11145951395019202No Hit
CCCAATGAACCTTGATGGGACCCCTGTTTGCAAATCGGACGCATCGTAGA15970.11118103921202788No Hit
TCCCTACACGGACGGATTTGTGTACACTTTTGGCCAGGGGACCACGCTGG15920.11083294578932272No Hit
TCTTTACTTTGACCATCAGCAGCCTGCAGCCTGAAGATATCGCTACATATCACTGTCATCATTATGATAATCTCC15870.11048485236661756No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15750.10964942815212518No Hit
ACACAAATCCGTCCGTGTAGGGAGGGAGATTATCATAATGATGACAGTGA15730.10951019078304311No Hit
GTTGTGAATGTCCTGACTCGCCTGGCAAGTGATGGTGACT15540.10818743577676353No Hit
TCTACGATGCGTCCGATTTGCAAACAGGGGTCCCATCAAGGTTCATTGGG15430.10742163024681216No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15420.10735201156227114No Hit
TCTTCAGGCTGCAGGCTGCTGATGGTCAAAGTAAAGATTGTCCCAGATCCACTCCCAATGAACCTTGATGGGACC15270.10630773129415565No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15220.10595963787145049No Hit
CCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCA15100.10512421365695812No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14860.10345336522797335No Hit
CACCATCACTTGCCAGGCGAGTCAGGACATTCACAACCATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC14750.102687559698022No Hit
CATCGTAGATTAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATGGTTGTGAATGTCCTGA14470.1007382365308731No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG14460.10066861784633206No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14440.10052938047725002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)