FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436396
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92010.6405615164620342No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56550.3936936610795352No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46950.32685972392014456No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44110.30708801751049153No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42090.2930250432332031No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38270.2664307057385289No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36610.2548740041047176No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG35410.24651976195979383No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35400.24645014327525278No Hit
AAGTAAAGATTGTCCCAGATCCACTCCCAATGAACCTTGATGGGACCCCT34250.2384439945530341No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32690.22758347976463314No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32640.227235386341928No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32480.22612148738927149No Hit
CTTTTGGCCAGGGGACCACGCTGGACATCAAACGAACTGTGGCTGCACCA31180.21707105839893737No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCACAA30850.21477364180908326No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30630.21324203074918058No Hit
ATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCACAACCATTTAAATTGGTATCAGCAGAAA29890.20809024809314422No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCGTGGTCCCCTGGCCAAAAGTG29560.20579283150329017No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29240.20356503359797717No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT28450.19806515751923565No Hit
GGCCAAAAGTGTACACAAATCCGTCCGTGTAGGGAGGGAGATTATCATAATGATGACAGTGATATGTAGCGATAT28380.19757782672744842No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28180.19618545303662777No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28160.19604621566754574No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26910.18734388009991673No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26520.18462875140281648No Hit
ATCTTTACTTTGACCATCAGCAGCCTGCAGCCTGAAGATATCGCTACATATCACTGTCATCATTATGATAATCTC26380.18365408981924203No Hit
AGTCAGGACATTCACAACCATTTAAATTGGTATCAGCAGAAACCAGGGAA26290.18302752165837277No Hit
GTGTAGGGAGGGAGATTATCATAATGATGACAGTGATATGTAGCGATATC25950.18066048638397766No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGCGTGGTCC25870.18010353690764944No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25670.17871116321682878No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25670.17871116321682878No Hit
GTGATATGTAGCGATATCTTCAGGCTGCAGGCTGCTGATGGTCAAAGTAA24560.17098348923277423No Hit
CCCTACACGGACGGATTTGTGTACACTTTTGGCCAGGGGACCACGCTGGA23770.16548361315403273No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATGGTTGTGA23380.16276848445693248No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23090.16074954260524255No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23070.16061030523616052No Hit
ACATATCACTGTCATCATTATGATAATCTCCCTCCCTACACGGACGGATT22830.15893945680717575No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22370.15573699731828827No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22310.15531928521104207No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22120.15399653020476248No Hit
CTCCTAATCTACGATGCGTCCGATTTGCAAACAGGGGTCCCATCAAGGTTCATTGGGAGTGGATCTGGGACAAT21430.1491928409714313No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21320.14842703544147995No Hit
GTCCCAGATCCACTCCCAATGAACCTTGATGGGACCCCTGTTTGCAAATC21090.14682580569703618No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC20870.1452941946371335No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT20690.14404105831539493No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20640.14369296489268976No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20450.14237020988641017No Hit
GATTAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT20040.13951584382022786No Hit
GATATGTAGCGATATCTTCAGGCTGCAGGCTGCTGATGGTCAAAGTAAAG19600.13645262170042244No Hit
ATTCACAACCATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA19090.13290206878882982No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18910.13164893246709125No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACT18700.1301869400917296No Hit
GATTTGTGTACACTTTTGGCCAGGGGACCACGCTGGACATCAAACGAACT18610.12956037193086029No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18510.12886418508544997No Hit
CAGTGATATGTAGCGATATCTTCAGGCTGCAGGCTGCTGATGGTCAAAGT18130.12621867507289075No Hit
CCCCTAAACTCCTAATCTACGATGCGTCCGATTTGCAAACAGGGGTCCCA18100.12600981901926767No Hit
GTCCAGCGTGGTCCCCTGGCCAAAAGTGTACACAAATCCGTCCGTGTAGG18050.1256617255965625No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17880.12447820795936497No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17540.12211117268496989No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT17380.12099727373231337No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17270.12023146820236202No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17220.11988337477965687No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17100.11904795056516448No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17090.11897833188062344No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17060.11876947582700037No Hit
ACGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT16850.11730748345163869No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16680.11612396581444115No Hit
GCCTGAAGATATCGCTACATATCACTGTCATCATTATGATAATCTCCCTCCCTACACGGACGGATTTGTGTACA16670.1160543471299001No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16650.11591510976081804No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG16590.11549739765357185No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16510.11494044817724361No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTACGATGCGTCCGATTTGCAAACAGGGGTCCCATCAAGGTT16360.11389616790912813No Hit
GGGGACCACGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCA16300.11347845580188193No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT16280.11333921843279986No Hit
CTACATATCACTGTCATCATTATGATAATCTCCCTCCCTACACGGACGGATTTGTGTACACTTTTGGCCAGGGGA16090.11201646342652025No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15950.11104180184294582No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15930.11090256447386375No Hit
GTCTCCTATAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG15670.10909247867579691No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15650.10895324130671487No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15640.10888362262217383No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15640.10888362262217383No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC15480.10776972366951733No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15420.10735201156227114No Hit
GTTGTGAATGTCCTGACTCGCCTGGCAAGTGATGGTGACT15150.10547230707966326No Hit
TCCCTACACGGACGGATTTGTGTACACTTTTGGCCAGGGGACCACGCTGG15100.10512421365695812No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15030.1046368828651709No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14980.10428878944246574No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCAC14850.10338374654343231No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14810.1031052718052682No Hit
GTGTACACTTTTGGCCAGGGGACCACGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG14720.1024787036443989No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14710.10240908495985786No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14640.10192175416807063No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14600.10164327942990652No Hit
ACACAAATCCGTCCGTGTAGGGAGGGAGATTATCATAATGATGACAGTGA14600.10164327942990652No Hit
CACCATCACTTGCCAGGCGAGTCAGGACATTCACAACCATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC14580.10150404206082445No Hit
CCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCA14450.10059899916179103No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT200.008571316549.792231
TACGTAT2100.041.8925270
ATGGCAA5400.033.83626670
GTATCAA41900.032.0856861
TATCAAC42100.031.13612
GTACGTA2100.030.29910969
ATCAACG44250.029.6119273
AACGCAG44300.029.5056786
GGTACGT1950.029.29233668
TCAACGC44900.029.1852784
CGCAGAG45250.028.8872288
CAACGCA45250.028.8862195
AATGGTA11350.028.61921970
AGAGTAC45900.028.47418211
CAGAGTA45950.028.44517910
GCAGAGT46200.028.290279
TGTTAGA22650.028.08484670
ACGCAGA46750.027.9603657
GTGGTAT4750.027.9535311
TAGGGTG606.8184896E-427.6459715
Produced by FastQC (version 0.11.5)