FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980927
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48440.493818602199756No Hit
GAGTTATAGTCGTCCCCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT42520.4334675261257973No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40110.4088989292781216No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29060.29625038356574956No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27500.28034705946517935No Hit
CTATAACTCTGTAGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAAACTGGTGATGGTGAGAGTGAAATCTGTC27290.278206227374718No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27060.2758615065137365No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26010.26515734606142966No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAAGACCTATTTAAATTGGTATCACCAGAAA25710.2620990145036277No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24920.25404540806808257No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24540.25017152142820004No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24230.24701124548513806No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23310.23763236204121202No Hit
GTATGGGACAGATTTCACTCTCACCATCACCAGTTTGCAACCTGAAGATT22820.23263708716346884No Hit
GTTATAGTCGTCCCCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT21840.22264653740798243No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21800.2222387598669422No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAAACTGGTGATGGTGAGAGTGAAATCTGTCCCATACCCATTGCCA21460.21877265076809999No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21350.21765126253023925No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20880.2128598764230162No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20840.21245209888197591No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20450.20847626785683337No Hit
ACTTACTACTGTCTACAGAGTTATAGTCGTCCCCCGTGGACGTTCGGCCA20430.20827237908631327No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20230.20623349138111197No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAAGAC20050.20439849244643077No Hit
GTCCCATACCCATTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT18510.18869905711638074No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18180.18533489240279857No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18000.1834998934681174No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17880.18227656084499663No Hit
CTGCTACTCTGGCTCCGAGGTGGCAGATGTGACATCCAGATGACCCAGTC17210.17544628703257226No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16920.17248989986003035No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGGCAGATGTGACAT16760.17085878969586932No Hit
GGGTATGGGACAGATTTCACTCTCACCATCACCAGTTTGCAACCTGAAGA16020.1633149051866245No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTTGGCCGAACGTC15980.16290712764558424No Hit
GATTTCACTCTCACCATCACCAGTTTGCAACCTGAAGATTTTGCAACTTACTACTGTCTACAGAGTTATAGTCGT15930.1623974057192839No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15820.16127601748142317No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15310.15607685383315986No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14890.15179518965223712No Hit
AGTCAGAACATTAAGACCTATTTAAATTGGTATCACCAGAAACCAGGCAA14550.1483290805533949No Hit
CATTAAGACCTATTTAAATTGGTATCACCAGAAACCAGGCAAAGCCCCTA14550.1483290805533949No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14540.14822713616813482No Hit
GAGTGAAATCTGTCCCATACCCATTGCCACTGAACCTTGATGGGACCCCA14210.14486297145455268No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC14120.1439454719872121No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14100.14374158321669195No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13980.14251825059357118No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13720.13986769657680947No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA13490.13752297571582797No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGGCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13100.13354714469068543No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12900.13150825698548416No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12770.1301829799771033No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12750.12997909120658316No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12550.12794020350138186No Hit
GGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12480.1272265928045614No Hit
GTTTAGGGGCTTTGCCTGGTTTCTGGTGATACCAATTTAAATAGGTCTTA12210.12447409440253963No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12180.12416826124675945No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12140.12376048370571918No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12020.1225371510825984No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11980.12212937354155813No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11950.12182354038577795No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11840.12070215214791723No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11670.11896909759849612No Hit
CCCTGGTCCCTTGGCCGAACGTCCACGGGGGACGACTATAACTCTGTAGA11580.11805159813115552No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11220.11438160026179318No Hit
ATTTAAATTGGTATCACCAGAAACCAGGCAAAGCCCCTAAACTCCTGATC11190.11407576710601298No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11060.11275049009763215No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11010.11224076817133181No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG10910.11122132431873116No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT10840.11050771362191071No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC10810.1102018804661305No Hit
CACCATCACCAGTTTGCAACCTGAAGATTTTGCAACTTACTACTGTCTAC10710.10918243661352986No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10700.10908049222826978No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10590.10795910399040906No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10550.10755132644936882No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10540.10744938206410876No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG10470.10673577136728829No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10470.10673577136728829No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10410.10612410505572789No Hit
CCAGAAACCAGGCAAAGCCCCTAAACTCCTGATCTATGATGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT10410.10612410505572789No Hit
GTCTACAGAGTTATAGTCGTCCCCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTG10360.10561438312942757No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10260.1045949392768269No Hit
GACTATAACTCTGTAGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAAACTGGTGATGGTGAGAGTGAAAT10130.10326966226844607No Hit
GATCTATGATGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAATGGGTATGGGACAGATTTCA10110.10306577349792595No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10050.10245410718636555No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9920.1011288301779847No Hit
CATCAAGGTTCAGTGGCAATGGGTATGGGACAGATTTCACTCTCACCATCACCAGTTTGCAACCTGAAGATTTTG9860.1005171638664243No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCATCATAGATCAGGAGT9820.10010938632538406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25350.039.79951
TATCAAC27200.036.7109952
ATCAACG29200.034.3132553
TCAACGC29550.033.9068374
CAACGCA29850.033.5660635
TGTTAGA9900.032.50963670
AACGCAG30850.032.4780246
CGCAGAG32600.030.6269138
TATAAGA1251.4370016E-1029.9902522
AGAGTAC33000.029.94589211
CCCTAAG1600.029.87620728
CACGAGA1600.029.82745721
TCGTATG1401.4551915E-1129.60366440
CCGCACT704.0710533E-529.33090431
GCAGAGT34000.029.2655779
CTTATAC1750.029.2157521
ACGCAGA34850.029.0406847
TATACAC2100.027.5884363
GACAGGT1950.026.2192671
TGCGCTA659.3036547E-426.21258410
Produced by FastQC (version 0.11.5)