FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980927
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46950.4786288887960062No Hit
GAGTTATAGTCGTCCCCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT42950.43785113469198017No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30670.31266342959262006No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30030.3061389889359759No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27680.28218205839986055No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26540.27056039848021307No Hit
CTATAACTCTGTAGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAAACTGGTGATGGTGAGAGTGAAATCTGTC26060.26566706798773No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25090.25577846261750364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24550.25027346581346016No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAAGACCTATTTAAATTGGTATCACCAGAAA24130.2459918016325374No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23730.24191402622213476No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22560.22998653314670717No Hit
GTATGGGACAGATTTCACTCTCACCATCACCAGTTTGCAACCTGAAGATT20900.21306376519353631No Hit
GTTATAGTCGTCCCCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT20810.21214626572619572No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAAACTGGTGATGGTGAGAGTGAAATCTGTCCCATACCCATTGCCA20770.21173848818515548No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20590.20990348925047428No Hit
ACTTACTACTGTCTACAGAGTTATAGTCGTCCCCCGTGGACGTTCGGCCA20160.20551988068429147No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAAGAC19840.2022576603559694No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19590.19970905072446776No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19380.1975682186340064No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19340.19716044109296615No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19210.19583516408458532No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18990.19359238760886388No Hit
CTGCTACTCTGGCTCCGAGGTGGCAGATGTGACATCCAGATGACCCAGTC18900.19267488814152328No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18590.18951461219846125No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18470.18829127957534048No Hit
GTCCCATACCCATTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT18240.18594655871435897No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18140.1849271148617583No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17970.1831940603123372No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17940.182888227156557No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17170.175038509491532No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16290.16606740358864625No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTTGGCCGAACGTC16010.1632129608013644No Hit
GGGTATGGGACAGATTTCACTCTCACCATCACCAGTTTGCAACCTGAAGA15850.16158185063720337No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15690.15995074047304234No Hit
GATTTCACTCTCACCATCACCAGTTTGCAACCTGAAGATTTTGCAACTTACTACTGTCTACAGAGTTATAGTCGT15580.15882935223518163No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGGCAGATGTGACAT15400.15699435330050043No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14930.1522029671932774No Hit
AGTCAGAACATTAAGACCTATTTAAATTGGTATCACCAGAAACCAGGCAA14890.15179518965223712No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13890.14160075112623058No Hit
CATTAAGACCTATTTAAATTGGTATCACCAGAAACCAGGCAAAGCCCCTA13880.14149880674097054No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13610.13874630833894877No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13000.1325277008380848No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13000.1325277008380848No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12950.13201797891178446No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA12870.13120242382970393No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12840.13089659067392376No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGGCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12740.1298771468213231No Hit
GAGTGAAATCTGTCCCATACCCATTGCCACTGAACCTTGATGGGACCCCA12660.12906159173924256No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12640.12885770296872245No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12350.12590131579618055No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12210.12447409440253963No Hit
ATTTAAATTGGTATCACCAGAAACCAGGCAAAGCCCCTAAACTCCTGATC12030.12263909546785846No Hit
GTTTAGGGGCTTTGCCTGGTTTCTGGTGATACCAATTTAAATAGGTCTTA11870.12100798530369741No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11840.12070215214791723No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11820.12049826337739708No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAATGGGTATGGGACAGATTTCACT11790.12019243022161691No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11760.1198865970658367No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11720.11947881952479646No Hit
GGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11570.11794965374589547No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11320.11540104411439382No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC11280.11499326657335358No Hit
CTCCTGATCTATGATGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT11260.11478937780283344No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10910.11122132431873116No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10910.11122132431873116No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10640.1084688259167094No Hit
CCCTGGTCCCTTGGCCGAACGTCCACGGGGGACGACTATAACTCTGTAGA10590.10795910399040906No Hit
CACCATCACCAGTTTGCAACCTGAAGATTTTGCAACTTACTACTGTCTAC10540.10744938206410876No Hit
GTCTACAGAGTTATAGTCGTCCCCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTG10490.10693966013780842No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10310.10510466120312724No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10300.10500271681786717No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10230.10428910612104672No Hit
GATCTATGATGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAATGGGTATGGGACAGATTTCA10160.10357549542422627No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10130.10326966226844607No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10090.1028618847274058No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10020.10214827403058535No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC10000.10194438526006523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)