FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574669
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT108930.6917644279527951No Hit
CTATAATATTGCTGACAGTAATAAACAGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC100410.6376578188813014No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT94990.6032378868193887No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC92340.5864089532466823No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC85390.5422726934993958No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC73640.4676538370921127No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA66500.422310974560368No Hit
GTTTTATACAGTTTTGACGAGAAGAACTACTTAGCCTGGTATCAGCAGAA64940.4124041306458691No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC62420.3964007673993709No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT60180.3821755556247059No Hit
GTAATAAACAGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA56780.36058371632387504No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT55930.3551857564986673No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC55590.3530265725685843No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG55080.34978779667345966No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT53140.3374677471900444No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAA52150.33118071162892015No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51920.3297200872056286No Hit
GTATAAAACACTCCGGCTGGACCTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGACAGGGAGTCTGGAGA51470.32686234376875395No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG50900.3232425354153794No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT48890.31047794806400586No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT48690.3092078398698393No Hit
ATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGA47810.3036193638155066No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC46750.2968877903864241No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG46310.2940935523592577No Hit
GTCCTGGTTTCTGCTGATACCAGGCTAAGTAGTTCTTCTCGTCAAAACTG44450.2822815461535091No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCGCGGTT44420.28209102992438406No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG44020.27955081353605105No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTG41800.2654526125808027No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40790.2590385662002618No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG40680.2583400066934702No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC40560.2575779417769703No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAGGCTAAGTAGTTCTTC40340.2561808227633871No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40190.2552282416177622No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG39610.2515449278546793No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39480.2507193575284711No Hit
GTCAGGGACCCCGGATTCGCGGTTAGATGCCCAGTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTCTGCTGATA39370.2500207980216795No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA37680.23928838378097236No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA37340.2371291998508893No Hit
GAGTACTATAATATTGCTGACAGTAATAAACAGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT36590.23236629412276485No Hit
AACCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTAACCGCGAA35960.2283654533111403No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG34000.21591839300830842No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA33580.21325116580055872No Hit
GTCTCCAGACTCCCTGTCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAGGTCCAGCCGGAGTGTTTTATA33120.2103299169539757No Hit
GTCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAGGTCCAGCCGGA32940.20918681957922586No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT32390.20569402204526793No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32030.2034078272957682No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC32030.2034078272957682No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC32000.2032173110666432No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC31950.20289978401810158No Hit
GTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAGGCTA31740.20156617041422673No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC31530.20023255681035187No Hit
CTCCCTGTCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAGGTCCAGCCGGAGTGTTTTATACAGTTTTGA30800.1955966619016441No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC30380.1929294346938944No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCTGTTTATTACTGTCAGCAATATTATAGTACT30090.19108777781235295No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA30010.19057973453468635No Hit
TTATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT29100.18480074225122867No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC28990.18410218274443707No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT28840.1831496015988122No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC28600.18162547176581237No Hit
GAACTACTTAGCCTGGTATCAGCAGAAACCAGGACAGCCTCCTAAGTTAC28260.1794662878357293No Hit
AAGTAGTTCTTCTCGTCAAAACTGTATAAAACACTCCGGCTGGACCTGCA28140.17870422291922938No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27530.1748303929270215No Hit
CTCCTAAGTTACTCATTTACTGGGCATCTAACCGCGAATCCGGGGTCCCT27500.17463987669789652No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT26920.1709565629348136No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT26790.17013099260860537No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA26000.1651140652416476No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT25920.164606021963981No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC25430.161494256888273No Hit
CAGTAATAAACAGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA25340.1609227082008981No Hit
CTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAGGCTAAGTAGTTCTTCT24990.15870001886110668No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCTGTTTATTACTGTCAGCAATATTATAGTACTCCATTCACTTT24920.1582554809931484No Hit
GTACTATAATATTGCTGACAGTAATAAACAGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT24650.1565408349310236No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24650.1565408349310236No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT24520.15571526460481536No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC24050.15273051034852403No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT24000.1524129832999824No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA23740.15076184264756592No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG23720.15063483182814927No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGGTGACCCAGTCTCCAGACTCCCTGTCTGTGTCTCTGGGCGAGAGGG23210.14739605593302466No Hit
CCCTGTCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAGGTCCAGC23090.14663399101652475No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA22950.14574491528060818No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG22710.14422078544760836No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG22560.14326820430198348No Hit
CTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCA22550.14320469889227513No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT22440.14250613938548354No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT22240.14123603119131703No Hit
CTACGGGGACATCGTGGTGACCCAGTCTCCAGACTCCCTGTCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTG22230.14117252578160872No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGGTGA22140.14060097709423378No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22050.14002942840685884No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT21990.1396483959486089No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21790.13837828775444236No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA21780.13831478234473404No Hit
GGCTGAAGATGTGGCTGTTTATTACTGTCAGCAATATTATAGTACTCCAT21510.13660013628260922No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT21080.1338694036651512No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20970.13317084415835964No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG20870.13253579006127636No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20620.1309481548185682No Hit
GTTTTGACGAGAAGAACTACTTAGCCTGGTATCAGCAGAAACCAGGACAGCCTCCTAAGTTACTCATTTACTGGG20610.1308846494088599No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG20600.13082114399915157No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC20540.1304401115409016No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC20390.12948753039527672No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT20210.12834443302052687No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCC19820.12586772204190214No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG19680.12497864630598558No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCGCGGTTAGATGCCCAGTAAAT19340.12281946237590248No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19280.12243842991765254No Hit
CTCATTTACTGGGCATCTAACCGCGAATCCGGGGTCCCTGACCGATTCAG19280.12243842991765254No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19270.12237492450794421No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT19270.12237492450794421No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC19200.12193038663998593No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA19060.12104131090406936No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG18930.12021574057786112No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGG18800.1193901702516529No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA18600.11812006205748637No Hit
GTTAGATGCCCAGTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTCTGCT18570.11792954582836139No Hit
GGTTTCTGCTGATACCAGGCTAAGTAGTTCTTCTCGTCAAAACTGTATAA18360.11659593222448654No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17950.11399221042644517No Hit
GAATCGGTCAGGGACCCCGGATTCGCGGTTAGATGCCCAGTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTCTG17880.1135476725584869No Hit
GCCTCCTAAGTTACTCATTTACTGGGCATCTAACCGCGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT17840.11329365091965359No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17650.11208704813519539No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT17500.11113446698957051No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTGAATGGAGTA17340.11011838043423729No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC17300.10986435879540399No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGG17220.10935631551773739No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17020.10808620732357087No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT17020.10808620732357087No Hit
GCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTGAATGG16650.10573650716436278No Hit
ACTTAGCCTGGTATCAGCAGAAACCAGGACAGCCTCCTAAGTTACTCATT16470.10459340978961293No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG16280.10338680700515473No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGC16150.10256123667894648No Hit
TTATTACTGTCAGCAATATTATAGTACTCCATTCACTTTCGGCCCTGGGA15990.10154515012361327No Hit
GGTCCAGCCGGAGTGTTTTATACAGTTTTGACGAGAAGAACTACTTAGCCTGGTATCAGCAGAAACCAGGACAGC15880.10084659061682169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAA150.001901347372.8300558
TTCGTGA150.002399103368.6830140
GTGGTAT2400.044.5805661
GTCGTAA405.808779E-542.04689430
TATGGAC556.2521194E-638.569150
GACAGGT2350.038.4151651
GAGTTTA1650.036.4750061
GTATCAA20000.035.1071931
AATGGTA5250.034.85427570
TCGTAAA601.5778736E-533.6967931
GATTGCG701.626842E-533.5461567
ACATCGA601.6541384E-533.4652122
GGATTGC701.8098857E-533.0275466
CTATTGT751.7308303E-632.05206740
CTTACAG2001.1945303E-831.6704770
GGTCGTA553.8021686E-430.54732329
TTACACG553.8021686E-430.54732329
ATCGAGG553.8760772E-430.44713424
GCTTACA553.9145674E-430.395841
AGTTTAT2100.030.239562
Produced by FastQC (version 0.11.5)