FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062971
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACGACCATTGTTGTCTGCTGA60730.5713232063715755No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC59160.5565532832033987No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAG43470.4089481274653777No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA39700.37348149667300423No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTC36950.3476106121427584No Hit
CTATTACTGTCAGTCAGCAGACAACAATGGTCGTCCTTGGGTGTTCGGCG35910.33782671399313813No Hit
CCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35120.33039471443717655No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC34450.3240916262061712No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33320.3134610445628338No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33080.31120322191292143No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG32660.3072520322755748No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG32300.30386529830070624No Hit
GACTATTACTGTCAGTCAGCAGACAACAATGGTCGTCCTTGGGTGTTCGG31050.29210580533241265No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGT30360.2856145652139146No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACCGATGGTCAACGT30220.2842975020014657No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29250.2751721354580699No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28830.2712209458207232No Hit
CACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28450.267646059958362No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC27690.2604962882336395No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26980.2538168962276487No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26510.24939532687157034No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAGGCCACTGTCACGGCTC25980.24440930185301388No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACCGATGGTCAACGTGA25820.2429040867530723No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25810.24281001080932596No Hit
GTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25480.23970550466569643No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24880.2340609480409155No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC24370.2292630749098517No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24330.2288867711348663No Hit
CCCAAGGACGACCATTGTTGTCTGCTGACTGACAGTAATAGTCAGCCTCG24330.2288867711348663No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24120.226911176316193No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23520.22126661969141206No Hit
ATATAAAGACACTGAGAGGGCCTCGGGGATCCCTGAGCGATTCTCTGGCT23350.21966732864772417No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGA23190.2181621135477826No Hit
ACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACGACCAT21490.2021692031109033No Hit
CCATTGTTGTCTGCTGACTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACCGATGGTCAACGTGA21200.19944100074225918No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAACAC21150.19897062102352744No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT21050.19802986158606395No Hit
GGCCAGGCCCCTGTGATGGTGATATATAAAGACACTGAGAGGGCCTCGGG20640.1941727478924637No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA20600.1937964441174783No Hit
GGGCTGCTGTCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20370.19163269741131225No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAGTCAGCAGACAACAA20190.18993933042387798No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19970.1878696596614583No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19570.18410662191160437No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19360.18213102709293105No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19120.17987320444301866No Hit
CAACAATGGTCGTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC18870.17752130584935996No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18860.1774272299056136No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18610.17507533131195488No Hit
GGCCTGGCCTGGCTTCTGTTGGTACCAGTAAGCATATTGTTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC18500.17404049593074505No Hit
GCTCTGGAGATGCATTGCCAAAACAATATGCTTACTGGTACCAACAGAAG18430.1733819643245206No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18410.17319381243702792No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT17850.16792555958723238No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCCTGAAG17670.16623219259979813No Hit
GTCTTTATATATCACCATCACAGGGGCCTGGCCTGGCTTCTGTTGGTACC16820.1582357373813585No Hit
CCTCAGGGACAACAGTCACGTTGACCATCGGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAGTCAG16360.15390824396902644No Hit
GTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16180.15221487698159217No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAGGCCACTGTCA16170.1521208010378458No Hit
CAGTCACGTTGACCATCGGTGGAGTCCAGGCAGAAGACGAGGCTGACTAT16140.15183857320660676No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15900.1495807505566944No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGGACGACCATTGTTGTCTGCTGACTGACAGTAATAGTCAGCCTCGTCTT15890.14948667461294807No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15780.14845183923173821No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15740.1480755354567528No Hit
GACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACGACCA15260.14355989015692808No Hit
GTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCAAGGTCAGCCC14950.14064353590079126No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACGAC14890.14007908023831317No Hit
ACAATATGCTTACTGGTACCAACAGAAGCCAGGCCAGGCCCCTGTGATGGTGATATATAAAGACACTGAGAGGGC14740.13866794108211794No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAACAATATGCTTACTGGTACCAACAGAAGCCAGGCCAGGCCC14230.1338700679510542No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT14020.13189447313238084No Hit
AGACACACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC13950.1312359415261564No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13740.1292603467074831No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGACAGCAGCC13670.12860181510125865No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13530.12728475188880975No Hit
ACGTGACTGTTGTCCCTGAGGTGGAGCCAGAGAATCGCTCAGGGATCCCC13210.1242743216889266No Hit
CAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13000.1222987268702533No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC12960.12192242309526789No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCC12880.1211698155452971No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12850.12088758771405805No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12830.12069943582656535No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCCTGAAG12820.12060535988281901No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC12760.12004090422034092No Hit
GGACCAAGCTGACCGTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG12560.11815938534541395No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAACACC12430.11693639807671141No Hit
GGCCTGGAAGGCAGACAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12420.11684232213296505No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12130.11411411976432095No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC12060.1134555881580965No Hit
ATATATCACCATCACAGGGGCCTGGCCTGGCTTCTGTTGGTACCAGTAAG12040.11326743627060382No Hit
ACAGAAGCCAGGCCAGGCCCCTGTGATGGTGATATATAAAGACACTGAGAGGGCCTCGGGGATCCCTGAGCGATT11990.11279705655187207No Hit
CCATCACAGGGGCCTGGCCTGGCTTCTGTTGGTACCAGTAAGCATATTGT11860.11157406928316953No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC11840.11138591739567683No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT11780.11082146173319873No Hit
GCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCA11700.11006885418322797No Hit
GTAATAGACAGCCGTGTCCTCGTCTCTCAGGCTGCTCATATGCAGGAACA11620.10931624663325717No Hit
GTCTATTACTGTACGAGAGATGGGGTGGGAGCTACATTTGACCCCTCGAC11570.10884586691452543No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAGTCAGCAGAC11560.10875179097077907No Hit
CACTGAGAGGGCCTCGGGGATCCCTGAGCGATTCTCTGGCTCCACCTCAG11510.10828141125204732No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11490.10809325936455463No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCT11490.10809325936455463No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAACAATA11450.10771695558956923No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11430.10752880370207653No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC11380.1070584239833448No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT11370.10696434803959844No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11200.10536505699591052No Hit
CCCCTGTGATGGTGATATATAAAGACACTGAGAGGGCCTCGGGGATCCCT11160.10498875322092513No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGG11080.10423614567095434No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAGGCCACTG11070.10414206972720799No Hit
TCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT11050.10395391783971529No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11020.10367169000847624No Hit
GTGATATATAAAGACACTGAGAGGGCCTCGGGGATCCCTGAGCGATTCTC10990.10338946217723718No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG10900.10254277868352005No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT10820.10179017113354928No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG10780.10141386735856388No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT10730.10094348763983213No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTG10710.10075533575233944No Hit
TCACAGGGGCCTGGCCTGGCTTCTGTTGGTACCAGTAAGCATATTGTTTT10700.10066125980859308No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC10680.10047310792110038No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG10650.10019088008986134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTATA150.002477163768.1291352
AGGGGGG2500.057.63020770
TCTATAC252.7333092E-454.5033073
GGTATCA7500.054.0796431
TCGCTAA2250.050.14536731
TCGTATG2300.049.5252740
CTTATAC2450.048.6911541
CGTATGC2300.048.0863941
ACACTCG2450.047.3155727
CGCTAAT2350.046.59403232
GCTAATC2250.045.6621633
CACTCGC2550.045.51148628
TATACAC2700.045.4194223
ATCTCGT2300.044.8412837
AATCTCG2300.044.8008736
CTAATCT2300.044.71179234
TCTCCGA2600.044.54178211
ATGCCGT2500.044.41757244
TATGCCG2500.044.36441843
CTCGCTA2550.044.21668230
Produced by FastQC (version 0.11.5)