FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062971
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC56670.533128373210558No Hit
GCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACGACCATTGTTGTCTGCTGA54950.516947310886186No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAG47820.4498711629950394No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC35690.3357570432307184No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA35360.3326525370870889No Hit
CTATTACTGTCAGTCAGCAGACAACAATGGTCGTCCTTGGGTGTTCGGCG33830.3182589176938976No Hit
CCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33180.3121439813503849No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33050.3109209940816824No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG32840.308945399263009No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTC31830.29944372894462784No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31610.2973740581822082No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31120.2927643369386371No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30740.28918945107627586No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30230.2843915779452121No Hit
GACTATTACTGTCAGTCAGCAGACAACAATGGTCGTCCTTGGGTGTTCGG29380.2763951227267724No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGT29200.27470175573933814No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG29190.2746076797955918No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC27820.26171927550234203No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAACAC27200.2558865669900684No Hit
CACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25910.24375077024678943No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAGGCCACTGTCACGGCTC25440.23932920089071102No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25230.2373536060720377No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC25080.2359424669158425No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24470.2302038343473152No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24150.22719340414743208No Hit
GTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24080.22653487254120763No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACCGATGGTCAACGT23420.22032586025394862No Hit
ATATAAAGACACTGAGAGGGCCTCGGGGATCCCTGAGCGATTCTCTGGCT23270.2189147210977534No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22920.21562206306663118No Hit
GGGCTGCTGTCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22460.21129456965429916No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22310.2098834304981039No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACCGATGGTCAACGTGA22240.20922489889187945No Hit
CCCAAGGACGACCATTGTTGTCTGCTGACTGACAGTAATAGTCAGCCTCG21630.2034862663233522No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21470.20198105122341062No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21060.1981239375298103No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGA20850.196148342711137No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA20350.19144454552381956No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT20310.19106824174883416No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19810.18636444456151674No Hit
GGCCAGGCCCCTGTGATGGTGATATATAAAGACACTGAGAGGGCCTCGGG19580.1842006978553507No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAGTCAGCAGACAACAA19460.18307178653039452No Hit
GGCCTGGCCTGGCTTCTGTTGGTACCAGTAAGCATATTGTTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC19160.18024950821800406No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19140.18006135633051137No Hit
CCATTGTTGTCTGCTGACTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACCGATGGTCAACGTGA19130.17996728038676502No Hit
ACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACGACCAT19020.17893244500555516No Hit
GCTCTGGAGATGCATTGCCAAAACAATATGCTTACTGGTACCAACAGAAG18520.17422864781823777No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18390.17300566054953523No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18030.16961892657466668No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18000.16933669874342763No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17790.16736110392475428No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17550.16510328127484195No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT17410.16378621806239305No Hit
CAACAATGGTCGTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC17270.16246915484994415No Hit
GTCTTTATATATCACCATCACAGGGGCCTGGCCTGGCTTCTGTTGGTACC17010.1600231803125391No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCCTGAAG16690.15701275011265595No Hit
GTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16060.15108596565663596No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16020.15070966188165058No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15860.149204446781709No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACGAC15820.14882814300672362No Hit
CCTCAGGGACAACAGTCACGTTGACCATCGGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAGTCAG15600.14675847224430394No Hit
CAGTCACGTTGACCATCGGTGGAGTCCAGGCAGAAGACGAGGCTGACTAT15590.14666439630055758No Hit
GTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCAAGGTCAGCCC15550.1462880925255722No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGGACGACCATTGTTGTCTGCTGACTGACAGTAATAGTCAGCCTCGTCTT15490.1457236368630941No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAACACC15100.14205467505698652No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAACAATATGCTTACTGGTACCAACAGAAGCCAGGCCAGGCCC14970.14083168778828398No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAGGCCACTGTCA14770.138950168913357No Hit
ACAATATGCTTACTGGTACCAACAGAAGCCAGGCCAGGCCCCTGTGATGGTGATATATAAAGACACTGAGAGGGC14040.13208262501987353No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT13810.12991887831370752No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13610.12803735943878056No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC13390.12596768867636088No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC12940.12173427120777519No Hit
GACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACGACCA12850.12088758771405805No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAGTCAGCAGAC12850.12088758771405805No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC12840.12079351177031171No Hit
AGACACACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC12630.11881791695163837No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12440.11703047402045776No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12420.11684232213296505No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGACAGCAGCC12400.11665417024547237No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC12400.11665417024547237No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCC12340.11608971458299426No Hit
GGACCAAGCTGACCGTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG12320.11590156269550157No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12320.11590156269550157No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC12310.11580748675175521No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCCTGAAG12160.11439634759555999No Hit
CAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12110.11392596787682825No Hit
GGCCTGGAAGGCAGACAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12030.11317336032685746No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC12010.11298520843936477No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT11870.11166814522691587No Hit
ACGTGACTGTTGTCCCTGAGGTGGAGCCAGAGAATCGCTCAGGGATCCCC11740.11044515795821333No Hit
ACAGAAGCCAGGCCAGGCCCCTGTGATGGTGATATATAAAGACACTGAGAGGGCCTCGGGGATCCCTGAGCGATT11470.10790510747706193No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGG11300.106305816433374No Hit
GTGATATATAAAGACACTGAGAGGGCCTCGGGGATCCCTGAGCGATTCTC11200.10536505699591052No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11180.10517690510841783No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAACAATA11160.10498875322092513No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11120.10461244944593973No Hit
CTGTGATGGTGATATATAAAGACACTGAGAGGGCCTCGGGGATCCCTGAGCGATTCTCTGGCTCCACCTCAGGGA10930.10282500651475909No Hit
GCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCA10880.10235462679602735No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT10820.10179017113354928No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTGTCTGCCTTCCAGGCCACTG10810.10169609518980292No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCT10750.10113163952732483No Hit
CACTGAGAGGGCCTCGGGGATCCCTGAGCGATTCTCTGGCTCCACCTCAG10680.10047310792110038No Hit
GTACCAACAGAAGCCAGGCCAGGCCCCTGTGATGGTGATATATAAAGACA10670.10037903197735404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)