FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001254
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46850.4679132368010515No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39010.38961142727020315No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32930.3288875749809739No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32560.3251922089699517No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC31640.3160037313209236No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29520.294830282825337No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26920.26886284599112714No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26450.26416873240955846No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA26370.26336973435312117No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG24450.2441937809986277No Hit
GATTTTACACTGAAGATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTGCAAACT24120.24089791401582417No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA23950.23920004314589507No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23840.23810142081829386No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23690.23660329946247408No Hit
CCTCTTACCTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG23490.234605804321381No Hit
GGTAAGAGGAGTTTGCAGACCTTGCATGCAGTAATAAACTCCAACATCCT23360.23330743247967048No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23170.23140981209563205No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23140.23111018782446813No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23130.23101031306741346No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA22460.22431870434475165No Hit
GGTCTAGTCAGAGCCTCCTACATAGTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC22420.2239192053165331No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22050.2202238393055109No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21580.21552972572394216No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGGTAAGAGGAGTTTG21430.21403160436812238No Hit
GTTTATTACTGCATGCAAGGTCTGCAAACTCCTCTTACCTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAA21310.21283310728346652No Hit
GTCTGCAAACTCCTCTTACCTTTGGCCAGGGGACCAAGCTGGAGATCAAA20020.19994926362341625No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19630.19605414809828475No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCATGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA19580.1955547743130115No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19490.1946559014995196No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19240.19215903257315325No Hit
CTCCTACATAGTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCATGAT19180.19155978403082535No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18610.1858669228787101No Hit
GTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGGTA18410.18386942773761702No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC18270.18247118113885188No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18060.18037381124070417No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17910.17887568988488436No Hit
GTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCATGATCTATTTGGGTT17900.1787758151278297No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAGATCAGCAGAG17770.1774774432861192No Hit
AGCCAGGGCAGTCTCCACAGCTCATGATCTATTTGGGTTCTAATCGGGCC17590.17567969765913544No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17260.1723838306763319No Hit
ATGTAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA17230.17208420640516792No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17040.17018658602112952No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC16800.16778959185181783No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA16640.16619159573894338No Hit
TTATTACTGCATGCAAGGTCTGCAAACTCCTCTTACCTTTGGCCAGGGGA16600.16579209671072476No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT16490.16469347438312357No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15820.1580018656604618No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC15500.15480587343471286No Hit
GTTTGCAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGTAAAAT15470.1545062491635489No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14940.14921288703965227No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA14450.14431902394397425No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCA14360.14342015113048237No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA14110.14092328220411604No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14080.14062365793295206No Hit
GGCCAAAGGTAAGAGGAGTTTGCAGACCTTGCATGCAGTAATAAACTCCA13960.13942516084829623No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13960.13942516084829623No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13850.13832653852069504No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13770.1375275404642578No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13750.13732779095014852No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13700.13682841716487526No Hit
CTCCACAGCTCATGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT13660.13642891813665664No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT13530.13513054629494614No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13290.13273355212563445No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13290.13273355212563445No Hit
GCTCATGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGT13280.13263367736857978No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13250.13233405309741583No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13200.13183467931214257No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13170.13153505504097862No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13160.13143518028392395No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13080.13063618222748674No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13030.13013680844221345No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGAATCCATTACTATGT12940.12923793562872157No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12910.12893831135755762No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG12850.1283390628152297No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12790.12773981427290176No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12550.1253428201035901No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12520.12504319583242612No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA12340.12324545020544236No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT11910.11895083565209227No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGAATCCA11870.11855133662387367No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTGCAAACTCCTCTTACCTTTGGCCAGGGGA11850.11835158710976434No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11810.11795208808154574No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCATGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT11690.1167535909968899No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11680.11665371623983524No Hit
AGTCAGAGCCTCCTACATAGTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG11630.11615434245456197No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11620.11605446769750734No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11550.11535534439812475No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT11470.11455634634168753No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC11300.11285847547175842No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11240.1122592269294305No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11220.1120594774153212No Hit
CCTACATAGTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAG11220.1120594774153212No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11170.11156010363004792No Hit
ATCCATTACTATGTAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC11140.11126047935888395No Hit
GAAGATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGC10780.10766498810491644No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT10750.10736536383375248No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10730.10716561431964318No Hit
AGTCTCCACAGCTCATGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGAT10590.10576736772087802No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10480.10466874539327682No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCATGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA10380.1036699978227303No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG10350.10337037355156634No Hit
GATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTGCAAACTCCTCTTACCTTTGG10320.10307074928040239No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTGCAAAC10180.10167250268163723No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10080.10067375511109068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCTA804.9427348E-543.2059470
CGGTATC405.95328E-541.8702552
TATAAGA405.95328E-541.8702552
ATGGCAA1508.5310603E-1041.47770770
TCGTATG1350.035.82277740
TATACAC1700.035.4647983
GGTATCA11200.035.2976951
GTATCAA26300.034.904161
ACGCGTA1550.034.70498727
GACGCGT1600.033.57669426
ATACGGG502.2111773E-433.50291
CTCTTGG400.002997593733.5028951
CGTATGC1450.033.44180341
TGTTAGA14350.033.2399770
ATCTCGT1650.033.1940237
TAATCTC1650.033.06239735
CTTGAAA1800.032.1838157
GTAATCT1700.032.03135334
GATTCAC1900.031.7395881
TTATACA1800.031.6353032
Produced by FastQC (version 0.11.5)