FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004692190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001254
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45110.45053502907354176No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34630.34586628368026495No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32760.32718970411104475No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30030.2999238954351243No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC28210.28174668965117744No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28050.28014869353830296No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27870.2783509479113192No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA26330.2629702353249026No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25030.24998651690779763No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG24850.24818877128081387No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA24200.2416969120722614No Hit
GGTCTAGTCAGAGCCTCCTACATAGTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC22810.22781432084166453No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22490.22461832861591563No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22470.22441857910180632No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22370.22341983153125977No Hit
GGTAAGAGGAGTTTGCAGACCTTGCATGCAGTAATAAACTCCAACATCCT22270.22242108396071328No Hit
GATTTTACACTGAAGATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTGCAAACT22210.22182183541838535No Hit
GTTTATTACTGCATGCAAGGTCTGCAAACTCCTCTTACCTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAA22110.2208230878478388No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22020.21992421503434692No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21330.21303285679757583No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA21050.21023636360004552No Hit
GTCTGCAAACTCCTCTTACCTTTGGCCAGGGGACCAAGCTGGAGATCAAA20920.20893799175833505No Hit
CCTCTTACCTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG20730.20704037137429665No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20630.2060416238037501No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGGTAAGAGGAGTTTG20370.2034448801203291No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19830.19805164323937782No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCATGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA19180.19155978403082535No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18940.18916278986151366No Hit
GTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGGTA18880.18856354131918573No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18660.18636629666398336No Hit
CTCCTACATAGTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCATGAT18650.18626642190692871No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18380.18356980346645307No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18330.1830704296811798No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17730.17707794425790058No Hit
GTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCATGATCTATTTGGGTT16950.16928771320763764No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAGATCAGCAGAG16850.1682889656370911No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16830.16808921612298178No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16660.16639134525305266No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16550.1652927229254515No Hit
GTTTGCAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGTAAAAT16480.1645935996260689No Hit
ATGTAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA16380.16359485205552238No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC16360.16339510254141307No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16100.16079835885799207No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA15890.15870098895984436No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT15840.1582016151745711No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15780.15760236663224317No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC15760.15740261711813386No Hit
AGCCAGGGCAGTCTCCACAGCTCATGATCTATTTGGGTTCTAATCGGGCC15700.15680336857580593No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15540.15520537246293148No Hit
TTATTACTGCATGCAAGGTCTGCAAACTCCTCTTACCTTTGGCCAGGGGA15210.1519095054801279No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15210.1519095054801279No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC14900.14881338801143368No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14860.14841388898321506No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14350.14332027637342773No Hit
CTCCACAGCTCATGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT13960.13942516084829623No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13960.13942516084829623No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA13760.1374276657072032No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13680.13662866765076592No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA13500.13483092202378216No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13420.13403192396734495No Hit
GGCCAAAGGTAAGAGGAGTTTGCAGACCTTGCATGCAGTAATAAACTCCA13410.13393204921029028No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA13170.13153505504097862No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG13050.13033655795632276No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCA12950.12933781038577624No Hit
GCTCATGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGT12900.12883843660050298No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12900.12883843660050298No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT12860.12843893757228436No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12780.12763993951584715No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT12710.12694081621646455No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12680.1266411919453006No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12680.1266411919453006No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12580.12564244437475405No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12530.1251430705894808No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12510.1249433210753715No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12350.12334532496249702No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12220.12204695312078653No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTGCAAACTCCTCTTACCTTTGGCCAGGGGA12210.12194707836373188No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12160.1214477045784586No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGAATCCATTACTATGT12060.12044895700791208No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11870.11855133662387367No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11640.11625421721161663No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11510.11495584536990613No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11290.11275860071470376No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT11260.1124589764435398No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11100.11086098033066534No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11010.10996210751717346No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGAATCCA10720.10706573956258851No Hit
CCTACATAGTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAG10670.10656636577731525No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC10640.1062667415061513No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10590.10576736772087802No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTGCAAAC10430.10416937160800356No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10430.10416937160800356No Hit
GCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGAATCCATTACTATGTAGGAGGCTCTGA10400.1038697473368396No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10360.10347024830862099No Hit
GAAGATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGC10260.10247150073807446No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCATGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT10180.10167250268163723No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCATGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA10160.10147275316752791No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10150.10137287841047327No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10140.10127300365341861No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT10120.1010732541393093No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10080.10067375511109068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)