FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168929
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT5300.31374127592065304No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC4150.2456653386925868No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT4040.2391537273055544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.22731443387458639No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.22553853985994116No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA3460.2048197763557471No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG3450.20422781168419868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.19061262423858544No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGAT2960.175221542778327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2770.16397421401890735No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC2490.1473992032155521No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA2460.14562330920090688No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.14325545051471328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.14088759182851968No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC2330.13792776847077767No Hit
TCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAG2250.13319205109839044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.13141615708374524No Hit
GGTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCC2040.12076079299587401No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.1195768636527772No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG1970.1166170402950352No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.11128935825109958No Hit
CCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTA1870.11069739357955116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.11069739357955116No Hit
ATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1850.10951346423645437No Hit
GTATCAACGCAGAGTACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA1850.10951346423645437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1830.10832953489335756No Hit
GAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG1800.10655364087871237No Hit
GCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAAC1790.10596167620716396No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.10536971153561556No Hit
ACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCG1770.10477774686406716No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1750.10359381752097034No Hit
TGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCG1740.10300185284942195No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.10122595883477674No Hit
GTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGC1690.10004202949167995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCTG203.533636E-582.3392356
CATACCC205.8489917E-574.3863544
GCACGTG200.002393210868.6835166
ACATACC251.8656584E-458.8466843
TCTTCTG655.820766E-1154.46473350
TAACCAC250.00657723153.2103963
TAGTTAT250.00851762749.8442857
TCGTATG703.3469405E-1045.58522840
GCCGTCT601.3846329E-744.19440546
GACGTAG702.910383E-1143.8109526
ACACATC703.274181E-1143.318116
TACACAT703.274181E-1143.318115
TTTTGAA358.876041E-442.8958845
CATAGTT400.001136394640.79251555
AGACGTA756.184564E-1140.7681625
GAGACGT756.366463E-1140.69527424
CCGTCTT751.6496415E-840.66195347
TGTGAAC300.001188529240.4302373
GTATGCC752.4649125E-838.85443542
CGTATGC752.8094291E-838.28264641
Produced by FastQC (version 0.11.5)