FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168929
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10660.6310343398705965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.25040105606497404No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC4200.2486251620503288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.23737783329090917No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.22553853985994116No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT3440.20363584701265028No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA3380.2000840589833599No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG3300.19534834161097267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.18528494219464983No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2750.16279028467581055No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGAT2720.16101439066116535No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC2480.1468072385440037No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA2310.13674383912768087No Hit
TCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAG2160.12786436905445484No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.1260884750398096No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.12253668701051922No Hit
GGTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCC1960.1160250756234868No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.11484114628038998No Hit
ACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCG1920.11365721693729319No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.11306525226574476No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.11010542890800276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1820.10773757022180916No Hit
GTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGC1820.10773757022180916No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.10536971153561556No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG1780.10536971153561556No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC1770.10477774686406716No Hit
GAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG1750.10359381752097034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.10359381752097034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.10300185284942195No Hit
ATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1740.10300185284942195No Hit
TGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCG1720.10181792350632514No Hit
GTATCAACGCAGAGTACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA1720.10181792350632514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.10063399416322834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCTA255.2383007E-4100.6387670
CTGTCCG400.003384420962.89922370
GATAGGT150.00382579961.0293352
ATCGATG200.004284444759.30153753
AATAAGC200.004908672557.2998949
TCATAAC200.005248808356.3386247
ATTGCAT200.005857045354.79974742
TCAAATG250.006123654554.17992466
CCTGTCC300.00651415753.33350869
AACTGAC200.00929374348.76278332
TATAGCG200.00955147148.42648731
AAATGGC300.00976343648.14726668
GTATAGC200.00981447748.094830
GGGGTTT954.3121247E-544.1398170
TTGTATC300.001143823140.7465917
GCTTGTA300.00114884340.71034615
TCGAGCC450.001882875236.80900659
CTTGTAT350.002442460534.91528716
CAGGGTA350.002453146734.8842431
CCGCTAT500.002554772734.58841362
Produced by FastQC (version 0.11.5)