FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707553
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTACTGATCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT31810.4495776288136719No Hit
GTACAGGGGACTGGCCTGCCTTCTGCTGATACCAGCAAACATATTTTTCC29070.41085261457445593No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT20740.293122918000489No Hit
CCTATGAGGTGACTCAGCCACCCTCAGTGTCCGTGTCTCCAGGACAGACA19180.271075099674512No Hit
GACTTAGGACGGTGAACTTGGTCCCAGTTCCGAAGACTCGATTGCTGCTGTCCCACGCCTGACAGTAATAGTCAG17710.2502992708673414No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16560.23404607146037115No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG15860.22415281964743278No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC15580.22019551892225742No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA15120.2136942391594693No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC14660.20719295939668125No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC14570.20592096987787487No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14420.2038009873465309No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC13680.1933424068585675No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCAATCGAGTCTTCGGAACTGGGACCAAGTT13040.18429714805816666No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCAATCGAGTCTTCGGAACTGGGACCAAGTTCACCGTCCTAAGTCAG12450.17595855010154718No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTGCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC12440.1758172179327909No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11760.16620663045736503No Hit
CTCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAGATTGGGGGAA11620.16422798009477735No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11510.16267332623845845No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG11420.1614013367196521No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10790.15249741008800755No Hit
GTTTATTACTGTGCGAGAGATCATGTCAACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA10710.15136675273795744No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10610.14995343105039483No Hit
GTACTGATCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCG10490.14825744502531968No Hit
CTATTACTGTCAGGCGTGGGACAGCAGCAATCGAGTCTTCGGAACTGGGACCAAGTTCACCGTCCTAAGTCAGCC10480.1481161128565634No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10280.14528946948143814No Hit
GGATTCGTGGCCTCCTATGAGGTGACTCAGCCACCCTCAGTGTCCGTGTCTCCAGGACAGACAGCCAGCATCAC9900.13991884706870014No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9870.13949485056243138No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG9800.13850552538113753No Hit
CCCTCAGTGTCCGTGTCTCCAGGACAGACAGCCAGCATCACCTGCTCTGG9800.13850552538113753No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT9690.13695087152481863No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG9610.13582021417476853No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9360.13228690995586195No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT8760.12380697983048619No Hit
GCCTTGGGCTGACTTAGGACGGTGAACTTGGTCCCAGTTCCGAAGACTCG8730.12338298332421742No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGCAACACAGCCACTCTGA8700.1229589868179486No Hit
GATCAGTACAGGGGACTGGCCTGCCTTCTGCTGATACCAGCAAACATATT8620.12182832946789851No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8520.1204150077803359No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGCAACACAG8400.11871902175526072No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC8260.11674037139267307No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGAACTTGGTCCCAGTTCCGAAGACTCGATTGCTG8240.11645770705516054No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8220.116175042717648No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8100.11447905669257286No Hit
GGACAGCAGCAATCGAGTCTTCGGAACTGGGACCAAGTTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCA8030.11348973151127902No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7980.11278307066749771No Hit
CTACAACCCCTCCCTGAAGAATCGCGTCAACATATCAGCGGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAG7900.1116524133174476No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7890.11151108114869134No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7850.11094575247366628No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG7780.10995642729237243No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTAT7750.10953243078610365No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG7670.10840177343605356No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT7560.10684711957973465No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7460.10543379789217204No Hit
GAGTGGCTGTGTTGCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT7330.10359647969834063No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA7300.10317248319207183No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7230.10218315801077799No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7170.10133516499824041No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7160.10119383282948416No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7090.1002045076481903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAG950.056.779625
TCCATAC200.00810367550.501432
TCGTATG1100.050.384640
GAGACTA1100.049.01958524
CGTATGC1150.048.33101341
TCTCGTA1150.047.88411738
AGGTGAT10050.046.52276270
CTTGAAA1250.045.9590857
CCGTCTT1150.045.86884347
ACTAGGC1200.045.0717127
ACGAGAC1200.044.91237622
CACGAGA1200.044.8996821
GCCGTCT1200.043.87344446
TTCAATT650.001022405941.6993370
ATCTCGT1350.040.65800537
TAGGCAT1350.040.15491529
GACTAGG1350.039.99846626
CGAGACT1350.039.93340723
ATCTCCG1350.039.90236710
CTTATAC1450.039.4891241
Produced by FastQC (version 0.11.5)