FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004692194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707553
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTACTGATCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT30770.4348790832630206No Hit
GTACAGGGGACTGGCCTGCCTTCTGCTGATACCAGCAAACATATTTTTCC28850.40774330686181814No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT18210.25736587930515453No Hit
GACTTAGGACGGTGAACTTGGTCCCAGTTCCGAAGACTCGATTGCTGCTGTCCCACGCCTGACAGTAATAGTCAG17620.24902728134853502No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC16790.23729671134176522No Hit
CCTATGAGGTGACTCAGCCACCCTCAGTGTCCGTGTCTCCAGGACAGACA15790.22316349446613892No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15760.22273949795987014No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA15020.21228091747190672No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14770.20874761325300012No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC14410.20365965517777468No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG14220.20097434397140568No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13830.19546238938991145No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13150.18585180191448555No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC13080.18486247673319173No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12450.17595855010154718No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCAATCGAGTCTTCGGAACTGGGACCAAGTT11960.16903327383249028No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCAATCGAGTCTTCGGAACTGGGACCAAGTTCACCGTCCTAAGTCAG11480.16224932973218967No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTGCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC11320.15998801503208948No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11200.1582920290070143No Hit
CTCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAGATTGGGGGAA10870.15362806743805765No Hit
GTTTATTACTGTGCGAGAGATCATGTCAACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA10600.14981209888163854No Hit
CTATTACTGTCAGGCGTGGGACAGCAGCAATCGAGTCTTCGGAACTGGGACCAAGTTCACCGTCCTAAGTCAGCC10180.14387614779387553No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10170.14373481562511925No Hit
GTACTGATCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCG10100.14274549044382542No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10000.14133216875626278No Hit
CCCTCAGTGTCCGTGTCTCCAGGACAGACAGCCAGCATCACCTGCTCTGG9650.1363855428497936No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9580.13539621766849974No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG9520.13454822465596217No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG9350.1321455777871057No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG9250.1307322560995431No Hit
GGATTCGTGGCCTCCTATGAGGTGACTCAGCCACCCTCAGTGTCCGTGTCTCCAGGACAGACAGCCAGCATCAC9160.1294602665807367No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT8990.12705761971188023No Hit
GCCTTGGGCTGACTTAGGACGGTGAACTTGGTCCCAGTTCCGAAGACTCG8770.12394831199924246No Hit
GATCAGTACAGGGGACTGGCCTGCCTTCTGCTGATACCAGCAAACATATT8750.12366564766172992No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG8630.12196966163665478No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8510.12027367561157963No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGCAACACAGCCACTCTGA8340.11787102874272316No Hit
CTACAACCCCTCCCTGAAGAATCGCGTCAACATATCAGCGGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAG8340.11787102874272316No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT8340.11787102874272316No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGAACTTGGTCCCAGTTCCGAAGACTCGATTGCTG8330.1177296965739669No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8180.11560971404262295No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8120.11476172103008539No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA8110.11462038886132912No Hit
GGACAGCAGCAATCGAGTCTTCGGAACTGGGACCAAGTTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCA7890.11151108114869134No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7710.1089671021110786No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG7530.10642312307346587No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA7440.1051511335546595No Hit
GAGTGGCTGTGTTGCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT7430.10500980138590324No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC7420.10486846921714699No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGCAACACAG7380.10430314054212195No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG7320.10345514752958436No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC7280.1028898188545593No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7190.10161782933575295No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC7080.10006317547943404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)