FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023193
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38650.3777390971204846No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34130.33356365807819244No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32690.31949006687887815No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28730.28078769108076385No Hit
GTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27990.27355542893667173No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCA27310.2669095664258845No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26610.26006823737066226No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25950.2536178414043099No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25880.2529337084987876No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24800.2423785150993019No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23430.2289890568055098No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22370.21862932995045903No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22140.2163814646894574No Hit
GTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22090.2158927983283701No Hit
GACTTAGGACGGTCAGTCTGGTCCCTCCGCCGAACACGAAACGGAGGCTG21110.206314937651059No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21040.20563080474553674No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18780.1835430852243907No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18110.17699495598582088No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17720.1731833583693399No Hit
TCTCTGGGCTCCGGACTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT17560.17161962601386052No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17500.17103322638055576No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16940.16556016313637797No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16650.16272589824207162No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16010.15647096882015415No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTCTGGTCCCTC15310.14962963976493193No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15190.14845684049832242No Hit
GCCTTGGGCTGACTTAGGACGGTCAGTCTGGTCCCTCCGCCGAACACGAAACGGAGGCTGTTGGATATATATGAG15120.14777270759280017No Hit
GGACCAGACTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG15030.14689310814284304No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGTA15000.14659990832619066No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT14580.1424951108930573No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14520.14190871125975255No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14430.1410291118097954No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14270.13946537945431606No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG14270.13946537945431606No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14240.13917217963766365No Hit
CATGAAGGGAGTACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCACCA14160.13839031345992397No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14060.13741298073774938No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13910.1359469816544875No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13380.13076711822696208No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13310.13008298532143986No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13300.12998525204922237No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13240.12939885241591761No Hit
GTCTGGTCCCTCCGCCGAACACGAAACGGAGGCTGTTGGATATATATGAGCTGCAGAAATAATCAGCCTCGTCCT13230.12930111914370016No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT13210.12910565259926524No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13090.12793285333265572No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG13070.1277373867882208No Hit
AGGGAGTACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC12840.1254895215272192No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12820.1252940549827843No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGG12580.12294845644956523No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12530.12245979008847793No Hit
GTACCAACAGCACCCAGGCGAAGCCCCCAAACTCTTAATTTATGACGTCA12380.12099379100521603No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACC12170.11894139228864936No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12170.11894139228864936No Hit
GTACTGACATTGGTGGTTATAACCATGTCTCGTGGTACCAACAGCACCCA12010.11737765993316998No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11870.11600939412212555No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTACTGACATT11660.11395699540555887No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11570.11307739595560173No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT11480.1121977965056446No Hit
CGCATGAAGGGAGTACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC11420.11161139687233983No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCCAGAGAAA11290.11034086433351284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11060.10809299907251124No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG10940.10692019980590173No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC10900.10652926671703188No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG10760.10516100090598743No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10730.10486780108933506No Hit
GTTCATAGGTTCTCATCCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCT10720.10477006781711759No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG10280.10046980383954934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT1550.046.45014637
GTGGTAT3800.046.3240851
TGGTATC3800.045.422142
TCGTATG1900.045.4126440
GGTATCA14150.044.4983251
CGTATGC1950.044.34324641
GTATCAA40200.040.8416061
GCCGTCT2000.040.13876746
TCTCGTA1800.040.0761238
ATGCCGT2350.036.99130644
GTATGCC2450.036.7751542
AGGGGGG2950.036.10679670
TCTCCGA2400.035.25066811
CCGTCTT2300.034.957947
TATACAC2450.034.534643
ATGTCGT501.9310192E-434.28463437
TAGGCAT2400.033.96512629
TATCAAC48900.033.913162
CCCACGA2500.033.8439519
GGGGTAC400.002853133533.84229318
Produced by FastQC (version 0.11.5)