FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023193
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37180.36337230610451793No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36480.3565309770492957No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC32310.3157762025346147No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30670.2997479458909512No Hit
GTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28190.275510094381021No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26870.2626093024483162No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25580.2500017103322638No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCA25220.24648331253243524No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24460.23905558384390824No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23900.23358252059973045No Hit
GTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22910.22390692665020187No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22390.21882479649489392No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22080.21579506505615265No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20970.20494667184001455No Hit
GACTTAGGACGGTCAGTCTGGTCCCTCCGCCGAACACGAAACGGAGGCTG20130.19673707697374787No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19830.19380507880722406No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19440.18999348119074308No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19110.18676828320756692No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC17490.1709354931083383No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG17020.16634202931411768No Hit
TCTCTGGGCTCCGGACTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT16500.16125989915880973No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16460.1608689660699399No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16460.1608689660699399No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15480.15129110539262874No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15280.14933643994827955No Hit
GCCTTGGGCTGACTTAGGACGGTCAGTCTGGTCCCTCCGCCGAACACGAAACGGAGGCTGTTGGATATATATGAG15270.1492387066760621No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGTA15230.14884777358719223No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14560.1422996443486224No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14490.14161551144310017No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14050.13731524746553192No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTCTGGTCCCTC13900.13584924838227003No Hit
GGACCAGACTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG13900.13584924838227003No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13880.13565378183783508No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13600.1329172502157462No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13540.13233085058244143No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13440.13135351786026683No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13410.13106031804361445No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC13390.13086485149917954No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13150.12851925296596048No Hit
GTCTGGTCCCTCCGCCGAACACGAAACGGAGGCTGTTGGATATATATGAGCTGCAGAAATAATCAGCCTCGTCCT13090.12793285333265572No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12900.12607592116052396No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT12870.12578272134387158No Hit
CATGAAGGGAGTACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCACCA12680.12392578917173984No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12590.12304618972178269No Hit
GTACCAACAGCACCCAGGCGAAGCCCCCAAACTCTTAATTTATGACGTCA12430.12148245736630332No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTACTGACATT12420.12138472409408588No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT12350.12070059118856363No Hit
AGGGAGTACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC12270.11991872501082397No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12120.11845272592756206No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11940.11669352702764776No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11740.11473886158329856No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGG11700.11434792849442871No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11230.10975446470020808No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACC11160.10907033179468585No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11130.10877713197803347No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC10950.10701793307811919No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10890.10643153344481443No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG10850.10604060035594458No Hit
GTTCATAGGTTCTCATCCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCT10850.10604060035594458No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10660.10418366818381282No Hit
CGCATGAAGGGAGTACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC10540.1030108689172033No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCCAGAGAAA10390.1015448698339414No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG10320.10086073692841917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)