FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766921
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGAACAGGTCATCTATCAAAGCTGGAATCGGCCCTCAGGGATCCCT77970.4412760955356804No Hit
GTTCAGGGGGCTGGCCTGGCTTCAGTTGATACCAAGAAAGAGAATTATCA72650.41116722253003957No Hit
GCCTATGGATGAGGCTGACTATGTTTGTCAGGCGTGGGACAGATCCTCCC71380.403979578034332No Hit
TCCATAGGCTGGGCCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGACTTGGAGCCAGAGAATCGCTCAGGGATC69220.39175492282903424No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT66540.3765872950743129No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG58340.3301788817949416No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC57800.32712271799361714No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGTCACAAGGGAGGATCTGTCCCACGCCTGACAAACATAGT51870.2935615118049986No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50390.28518535916433163No Hit
CCCCAGGACAGACGGCCACCATCACCTGCTCTGGAGATATATTGGGTGAT49710.28133685659970087No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGCGATCCTGCAGCTCTAGTCTCC49440.2798087746990386No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGTCACAAGGGAGGATCTGTCCCACGCCTGA48400.2739228296001915No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT47410.2683198626310967No Hit
GGAGATATATTGGGTGATAATTCTCTTTCTTGGTATCAACTGAAGCCAGGCCAGCCCCCTGAACAGGTCATCTAT44300.25071862296050584No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT43140.24415353035025333No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG42940.24302161783124426No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACGGCCACCATCACCTGCTCTGG42780.24211608781603708No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT40230.2276842031986716No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG38870.21998719806941No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC38610.21851571179469825No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGCGATCCTGCAG37930.21466720923006746No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC37810.21398806171866203No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG37640.21302593607750434No Hit
GAGTGGCTGTGTTCCCAGACTTGGAGCCAGAGAATCGCTCAGGGATCCCT36930.20900764663502217No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA36360.20578169595584636No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC35840.2028387234064228No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG35630.20165021526146332No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC35490.20085787649815695No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC35070.19848086020823794No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGCGATCCTGCAG33980.19231193697963858No Hit
CAGCCACTCTGACCATCAGCGGGGCCCAGCCTATGGATGAGGCTGACTATGTTTGTCAGGCGTGGGACAGATCCT33940.19208555447583678No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA32800.18563365311748517No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA32550.18421876246872385No Hit
GTCCCACGCCTGACAAACATAGTCAGCCTCATCCATAGGCTGGGCCCCGC32180.18212472430855708No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGCGAT32060.18144557679715168No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG31710.1794647298888858No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG31380.17759707423252086No Hit
GGATCCGTGGCCTCCGATGAGTTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACGGCCACCATCAC31280.17703111797301635No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30760.17408814542359277No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT30400.1720507028893765No Hit
ATATATCTCCAGAGCAGGTGATGGTGGCCGTCTGTCCTGGGGACACGGACACTGAGGGTGGCTGAGTCAACTCAT30000.16978687785135838No Hit
GGACAGATCCTCCCTTGTGACTTTCGGCGGAGGGACCAAGCTGACCGTCC29640.1677494353171421No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC28230.15976945205812823No Hit
GCACAGGATCCGTGGCCTCCGATGAGTTGACTCAGCCACCCTCAGTGTCC27680.15665669263085333No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA27080.15326095507382617No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCGATGAGTTGACT27050.1530911681959748No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26660.15088393878390716No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26500.14997840876869992No Hit
GATCTGTCCCACGCCTGACAAACATAGTCAGCCTCATCCATAGGCTGGGC26430.14958223938704673No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC26030.14731841434902862No Hit
GATCCTCCCTTGTGACTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCA24880.14080991736472656No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGCGATCCTGCAGCTCTAGT24580.13911204858621296No Hit
GTAATACACCGCCGTGTCTTCAGCTCTCAACCTGCTCAACTCCAAGTACACTGTGCTCGCGGATCTGTCGATGGT23850.1349805678918299No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTG23590.13350908161711814No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGTCACAAGGGAGGATC23280.1317546172126541No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC22990.13011334406009098No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT22980.13005674843414053No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG22740.12869845341132966No Hit
GCTTGGTCCCTCCGCCGAAAGTCACAAGGGAGGATCTGTCCCACGCCTGA22590.12784951902207287No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22420.12688739338091515No Hit
GTCCTGGGGACACGGACACTGAGGGTGGCTGAGTCAACTCATCGGAGGCCACGGATCCTGTGCAGTAAGCAAGGA22190.12558569398405475No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG22080.12496314209859978No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG21830.12354825144983844No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG21760.12315208206818529No Hit
ACTGAAGCCAGGCCAGCCCCCTGAACAGGTCATCTATCAAAGCTGGAATCGGCCCTCAGGGATCCCTGAGCGATT21730.1229822951903339No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC21670.1226427214346312No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC21460.1214542132896717No Hit
GGACAGACGGCCACCATCACCTGCTCTGGAGATATATTGGGTGATAATTC21440.1213410220377708No Hit
GTGTATTACTGTGCGAGAGATTCGATACTGCCGCGGGGCGGTATGGACGT21400.12111463953396898No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAAGTCTGGGAACACAGCCACT21340.12077506577826626No Hit
GCTCTGGAGATATATTGGGTGATAATTCTCTTTCTTGGTATCAACTGAAG21330.12071847015231582No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21320.12066187452636536No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC21140.1196431532592572No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20880.11817166698454543No Hit
GGATGAGGCTGACTATGTTTGTCAGGCGTGGGACAGATCCTCCCTTGTGACTTTCGGCGGAGGGACCAAGCTGAC20780.11760571072504089No Hit
GGCCAGCCCCCTGAACAGGTCATCTATCAAAGCTGGAATCGGCCCTCAGG20780.11760571072504089No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20690.11709635009148683No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC20560.11636060695413095No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20510.11607762882437868No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG19770.11188955250404518No Hit
GTGTCCGTGTCCCCAGGACAGACGGCCACCATCACCTGCTCTGGAGATAT19510.11041806622933341No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT19410.10985210996982887No Hit
GAGTTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACGGCCAC19390.10973891871792797No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC19210.10872019745081982No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT19200.10866360182486935No Hit
GACCATCAGCGGGGCCCAGCCTATGGATGAGGCTGACTATGTTTGTCAGGCGTGGGACAGATCCTCCCTTGTGA19170.10849381494701801No Hit
CCATCACCTGCTCTGGAGATATATTGGGTGATAATTCTCTTTCTTGGTAT19110.1081542411913153No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA19010.10758828493181076No Hit
GCCTCATCCATAGGCTGGGCCCCGCTGATGGTCAGAGTGGCTGTGTTCCC18840.10662615929065307No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC18820.10651296803875217No Hit
CATCCATAGGCTGGGCCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAC18510.10475850363428811No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAGTCTGGGAACACAG18340.10379637799313042No Hit
GACTATGTTTGTCAGGCGTGGGACAGATCCTCCCTTGTGACTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT18190.10294744360387362No Hit
CCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCGA18180.10289084797792318No Hit
GGTCATCTATCAAAGCTGGAATCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAGTCTGGGAACACAGC17970.10170233983296367No Hit
CTGATGGTCAGAGTGGCTGTGTTCCCAGACTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGATTCCAG17820.10085340544370687No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT17790.10068361856585553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)