FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766921
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGAACAGGTCATCTATCAAAGCTGGAATCGGCCCTCAGGGATCCCT74230.42010933143021106No Hit
GTTCAGGGGGCTGGCCTGGCTTCAGTTGATACCAAGAAAGAGAATTATCA72430.4099221187591296No Hit
GCCTATGGATGAGGCTGACTATGTTTGTCAGGCGTGGGACAGATCCTCCC67430.38162430578390316No Hit
TCCATAGGCTGGGCCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGACTTGGAGCCAGAGAATCGCTCAGGGATC65030.36804135555579454No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT63480.35926903353347434No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG63220.35779754725876256No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC50730.287109610446647No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGTCACAAGGGAGGATCTGTCCCACGCCTGACAAACATAGT49200.2784504796762277No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGCGATCCTGCAGCTCTAGTCTCC48240.2730172995849843No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGTCACAAGGGAGGATCTGTCCCACGCCTGA46800.2648675294481191No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46760.2646411469443173No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45120.25535946428844297No Hit
CCCCAGGACAGACGGCCACCATCACCTGCTCTGGAGATATATTGGGTGAT44890.2540577648915826No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGCGATCCTGCAG43770.24771905478513187No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG43760.24766245915918145No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT43050.24364416971669928No Hit
GGAGATATATTGGGTGATAATTCTCTTTCTTGGTATCAACTGAAGCCAGGCCAGCCCCCTGAACAGGTCATCTAT42200.23883354151091077No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACGGCCACCATCACCTGCTCTGG40360.2284199463360275No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT40350.22836335071007705No Hit
GAGTGGCTGTGTTCCCAGACTTGGAGCCAGAGAATCGCTCAGGGATCCCT38850.21987400681750907No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38700.2190250724282523No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC37180.21042253728378346No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC36780.20815871224576535No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG35740.2022727671469183No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC35360.20012213336080106No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT35290.19972596397914794No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGCGATCCTGCAG35250.1994995814753461No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC34890.19746213894112982No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG33310.18852003004095824No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA33060.18710513939219695No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC31790.17991749489648942No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG31770.17980430364458852No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA31720.17952132551483627No Hit
GGATCCGTGGCCTCCGATGAGTTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACGGCCACCATCAC31700.17940813426293536No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31590.17878558237748038No Hit
CAGCCACTCTGACCATCAGCGGGGCCCAGCCTATGGATGAGGCTGACTATGTTTGTCAGGCGTGGGACAGATCCT31350.1774272873546695No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG31090.17595580107995773No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA30310.1715413422558224No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT29660.167862626569043No Hit
GTCCCACGCCTGACAAACATAGTCAGCCTCATCCATAGGCTGGGCCCCGC28750.16271242460755178No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA28560.1616371077144932No Hit
GGACAGATCCTCCCTTGTGACTTTCGGCGGAGGGACCAAGCTGACCGTCC28390.16067498207333547No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGCGAT27900.1579017964017633No Hit
ATATATCTCCAGAGCAGGTGATGGTGGCCGTCTGTCCTGGGGACACGGACACTGAGGGTGGCTGAGTCAACTCAT27690.15671328825680378No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCGATGAGTTGACT27690.15671328825680378No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC27210.15399669821118206No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26670.15094053440985758No Hit
GATCTGTCCCACGCCTGACAAACATAGTCAGCCTCATCCATAGGCTGGGC26530.15014819564655124No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC26400.14941245250919538No Hit
GCACAGGATCCGTGGCCTCCGATGAGTTGACTCAGCCACCCTCAGTGTCC25800.1460167149521682No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGCGATCCTGCAGCTCTAGT25510.14437544179960507No Hit
GATCCTCCCTTGTGACTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCA24810.14041374798307338No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23650.13384865537282084No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG23640.13379205974687042No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC23220.13141504345695137No Hit
GTCCTGGGGACACGGACACTGAGGGTGGCTGAGTCAACTCATCGGAGGCCACGGATCCTGTGCAGTAAGCAAGGA23210.13135844783100092No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23090.1306793003195955No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22770.128868240289181No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTG22630.12807590152587467No Hit
GCTCTGGAGATATATTGGGTGATAATTCTCTTTCTTGGTATCAACTGAAG22490.12728356276256833No Hit
GTAATACACCGCCGTGTCTTCAGCTCTCAACCTGCTCAACTCCAAGTACACTGTGCTCGCGGATCTGTCGATGGT22420.12688739338091515No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGTCACAAGGGAGGATC22380.12666101087711334No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22300.1262082458695097No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT21830.12354825144983844No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG21570.12207676517512667No Hit
GCTTGGTCCCTCCGCCGAAAGTCACAAGGGAGGATCTGTCCCACGCCTGA21370.12094485265611762No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC21020.11896400574785176No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20940.11851124074024814No Hit
GTGTATTACTGTGCGAGAGATTCGATACTGCCGCGGGGCGGTATGGACGT20720.11726613696933817No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAAGTCTGGGAACACAGCCACT20680.11703975446553637No Hit
GGACAGACGGCCACCATCACCTGCTCTGGAGATATATTGGGTGATAATTC20600.11658698945793276No Hit
GGATGAGGCTGACTATGTTTGTCAGGCGTGGGACAGATCCTCCCTTGTGACTTTCGGCGGAGGGACCAAGCTGAC20530.11619082007627958No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC20140.11398359066421193No Hit
GGCCAGCCCCCTGAACAGGTCATCTATCAAAGCTGGAATCGGCCCTCAGG20100.11375720816041011No Hit
GACCATCAGCGGGGCCCAGCCTATGGATGAGGCTGACTATGTTTGTCAGGCGTGGGACAGATCCTCCCTTGTGA20030.11336103877875693No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC19830.11222912625974787No Hit
GAGTTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACGGCCAC19720.1116065743742929No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19710.11154997874834247No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT19600.11092742686288748No Hit
ACTGAAGCCAGGCCAGCCCCCTGAACAGGTCATCTATCAAAGCTGGAATCGGCCCTCAGGGATCCCTGAGCGATT19580.11081423561098656No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC19550.11064444873313523No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG19090.10804104993941438No Hit
GGCGTGGGACAGATCCTCCCTTGTGACTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGC19080.10798445431346392No Hit
GTGTCCGTGTCCCCAGGACAGACGGCCACCATCACCTGCTCTGGAGATAT19050.10781466743561258No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGT18960.1073053068020585No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA18730.10600360740519808No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18710.10589041615329717No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT18700.10583382052734673No Hit
CTGATGGTCAGAGTGGCTGTGTTCCCAGACTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGATTCCAG18540.10492829051213948No Hit
GCCTCATCCATAGGCTGGGCCCCGCTGATGGTCAGAGTGGCTGTGTTCCC18480.10458871675643677No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG18410.10419254737478359No Hit
GTCTGGGAACACAGCCACTCTGACCATCAGCGGGGCCCAGCCTATGGATG18290.10351339986337815No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGCGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC18230.10317382610767543No Hit
GGCTGGGCCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGACTTGGAGCC17970.10170233983296367No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17930.10147595732916187No Hit
GGTCATCTATCAAAGCTGGAATCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAGTCTGGGAACACAGC17860.10107978794750869No Hit
CCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCGA17850.10102319232155824No Hit
GACTATGTTTGTCAGGCGTGGGACAGATCCTCCCTTGTGACTTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT17770.10057042731395462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)