FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841918
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT90080.48905543026345366No Hit
GTAATGGATACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT88680.4814546575906202No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC84010.456100651603383No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC62660.34018886834267326No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG61230.3324252219697077No Hit
GTAATATACGCCCGTGTCTTCGGCGCTCAGGCTGTTCATTTGCAGGGACA58370.3168979292237765No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG58080.31532348345583244No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGTCTGTGCCTGATCCACTGCCA57090.30994865135147165No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56270.30549677021452637No Hit
GTGTAAAGTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG53700.2915439232365393No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC51250.27824257105908085No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT49570.2691216438516807No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT49350.2679272367173783No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC49120.2666785383496985No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA48560.26363822928056513No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG48180.26157516241222467No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG47650.2586977270432234No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG46620.25310573000535314No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTAGAAACT45840.24887101380191737No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT45180.24528779239901013No Hit
GTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGGTGGCCGATAAGAACCCAAATAGATCAGGAG44310.24056445509517796No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTC43110.2340495070898922No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG42900.23290939118896714No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG42380.23008624705334332No Hit
GGCACAGACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGG42150.22883754868566353No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT41870.22731739415109684No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG41270.22405992014845394No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41210.22373417274818966No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40380.21922800037786697No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40200.21825075817707412No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40150.21797930201018723No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC38910.21124718907139187No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34820.1890420746200428No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG34610.18790195871911777No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA34530.18746762885209872No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTTATCGGCCA34030.18475306718322967No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACGCAGTCTCCACTCTCCCTGCCCGTCACCC33720.18307003894853083No Hit
GTCAGGGACCCCGGGTGGCCGATAAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA33010.17921536137873673No Hit
GATCAGGACTGAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC32740.17774949807754742No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACGCAGTCTCCACTCTCC32600.1769894208102641No Hit
GTTTATTACTGCATGCAAGCTCTAGAAACTCTTATCTACACTTTTGGCCA32130.17443773284152714No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG31690.17204891857292237No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGTCTGTGCCTGATCCACTGCCA31110.16890002703703422No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGCGTCATCACAATATCC30680.1665655040018068No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGACTTTACACTGAA30120.16352519493267345No Hit
GTATATTACTGTGCAAGCGTGGGTGTTTTAAGGGGGTATTCTACACGGGGGCGAATCTCCTTCGGTCTGGACGT29990.16281940889876748No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29310.15912760502910553No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29060.157770324194671No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29010.1574988680277841No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA28750.15608729595997214No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA28690.15576154855970786No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACGCAGTCTCCACT28040.15223261839017807No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27990.15196116222329115No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27990.15196116222329115No Hit
GTTCAGTGGCAGTGGATCAGGCACAGACTTTACACTGAAAATCAGCAGAG27850.15120108495600781No Hit
GGATACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTTAT27030.14674920381906253No Hit
GCCCTGGCTTCTGCAGGTACCAATTCAAATAGGTGTATCCATTACTATGC26850.14577196161826966No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26750.14522904928449584No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25950.1408857506143053No Hit
TTATTACTGCATGCAAGCTCTAGAAACTCTTATCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA25880.14050571198066364No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25650.13925701361298387No Hit
GGCCAAAAGTGTAGATAAGAGTTTCTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTG25560.1387683925125874No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA25540.13865981004583264No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG25400.13789973277854933No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25390.13784544154517192No Hit
GGACTGAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT25270.13719394674464336No Hit
GCATAGTAATGGATACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGC25250.1370853642778886No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25150.13654245194411477No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24950.13545662727656713No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24520.13312210424133972No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG24480.1329049393078302No Hit
CTAGAAACTCTTATCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24440.13268777437432067No Hit
ATTCTACACGGGGGCGAATCTCCTTCGGTCTGGACGTCTGGGGCCAAGGGACCACGGTCGCCGTCTCCTCAGCAT24300.13192769710703733No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACGCAGTCTC23790.12915884420479087No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23630.12829018447075277No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23500.1275843984368468No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTAGAA23440.12725865103658252No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT23390.12698719486969562No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23230.12611853513565752No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23210.12600995266890275No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC23110.12546704033512893No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT23100.12541274910175154No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23080.12530416663499677No Hit
CTCCTGCATAGTAATGGATACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT22840.12400117703393963No Hit
GTGTAGAATACCCCCTTAAAACACCCACGCTTGCACAGTAATATACGCCC22580.1225896049661277No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22510.12220956633248602No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC22500.12215527509910865No Hit
GAGTTTCTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT22420.1217209452320896No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA21990.11938642219686219No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21910.11895209232984313No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA21890.11884350986308836No Hit
CTCCTGTGCAGCCTCTGGATTCGGTTTTAACAGTTATGGCATGGCCTGGGTCCGCCAGGCTCCAGGGAAGGGGCT21740.11802914136242765No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21580.11716048162838955No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21490.1166718605279931No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG21270.11547745339369071No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT21200.11509741476004903No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21060.11433733749276569No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20770.11276289172482162No Hit
GGGATATTGTGATGACGCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT20450.1110255722567454No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA20390.11069982485648112No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20390.11069982485648112No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTTATCGGCCACCCGGGGTCCCTGACAGGTT20350.1104826599229716No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTTATCGGCCACCCGGGGTCCCT20160.10945112648880134No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20150.10939683525542396No Hit
GTGTAGATAAGAGTTTCTAGAGCTTGCATGCAGTAATAAACCCCAACATC20100.10912537908853706No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20040.10879963168827277No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG20030.1087453404548954No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19820.10760522455397037No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19820.10760522455397037No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19620.10651939988642273No Hit
GGTGTATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGG19470.10570503138576201No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19430.10548786645225249No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19400.10532499275212036No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19210.10429345931795009No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19080.10358767328404414No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18770.10190464504934531No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18710.10157889764908101No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18600.10098169408192981No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18540.10065594668166551No Hit
ATTTGGGTTCTTATCGGCCACCCGGGGTCCCTGACAGGTTCAGTGGCAGT18500.10043878174815599No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18430.10005874311451433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCA200.00472634657.8794568
GGTATCA8600.047.519891
TACGTAT555.5048644E-447.29324370
CGTATGC2900.046.43111841
TAGCGCT2950.046.2780729
CTAGCGC2950.046.2541528
TCGTATG2950.044.4163140
ACTAGCG3200.042.62199427
CGAGACT3200.042.57105623
TCTGGAG19650.042.35934470
ACGAGAC3300.042.30845322
ATCTCCG3150.042.14501610
ATGCCGT3200.041.1966444
GACTAGC3400.040.1006426
TCTCCGA3350.039.62889511
AGACTAG3450.039.50443625
ATCTCGT2900.039.06735637
CGCTCAT3450.038.65442332
GTATCAA23450.038.485371
CACGAGA3750.038.1333121
Produced by FastQC (version 0.11.5)