FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841918
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT88280.47928300825552494No Hit
GTAATGGATACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT81340.44160489229162214No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71260.3868793290472214No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC60740.329764951534216No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC59300.321947013927873No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGTCTGTGCCTGATCCACTGCCA54950.298330327408712No Hit
GTGTAAAGTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG54210.29431277613878576No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG53720.29165250570329404No Hit
GTAATATACGCCCGTGTCTTCGGCGCTCAGGCTGTTCATTTGCAGGGACA52950.28747208073323566No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52110.28291161712953566No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT50480.27406214608902246No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC49630.269447391251945No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT47850.25978355171077105No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC46200.2508254982035031No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG45730.24827381023476616No Hit
GTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGGTGGCCGATAAGAACCCAAATAGATCAGGAG45550.24729656803397326No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44670.2425189394967637No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTC43930.23850138822683747No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT42550.2310091980207588No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTAGAAACT42350.22992337335321117No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG42150.22883754868566353No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42070.22840321881864448No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG41290.2241685026152087No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40330.21895654421098007No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40270.2186307968107158No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40010.21721922474290384No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39600.2149932841744312No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39320.2134731296398645No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39270.2132016734729776No Hit
GGCACAGACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGG37120.20152905829684056No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36220.19664284729287623No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC35700.1938197031572524No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA34670.18822770611938208No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG33890.18399298991594631No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33720.18307003894853083No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT33480.18176704934747367No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACGCAGTCTCCACTCTCC32110.17432915037477237No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA31700.17210320980629973No Hit
GATCAGGACTGAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC31690.17204891857292237No Hit
GTTTATTACTGCATGCAAGCTCTAGAAACTCTTATCTACACTTTTGGCCA31380.17036589033822352No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTTATCGGCCA31230.1695515218375628No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30950.16803136730299612No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACGCAGTCTCCACTCTCCCTGCCCGTCACCC30940.16797707606961873No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG30390.16499105823386276No Hit
GTCAGGGACCCCGGGTGGCCGATAAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA29950.16260224396525796No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA29660.1610277981973139No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGCGTCATCACAATATCC29610.16075634203042696No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC29450.1598876822963889No Hit
GTATATTACTGTGCAAGCGTGGGTGTTTTAAGGGGGTATTCTACACGGGGGCGAATCTCCTTCGGTCTGGACGT28360.15396993785825427No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGTCTGTGCCTGATCCACTGCCA27880.15136395865613994No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27570.14968093042144112No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGACTTTACACTGAA27260.1479979021867423No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACGCAGTCTCCACT27160.14745498985296848No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27100.1471292424527042No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA26930.14620629148528871No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26840.14571767038489225No Hit
GTTCAGTGGCAGTGGATCAGGCACAGACTTTACACTGAAAATCAGCAGAG26560.14419751585032559No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26390.1432745648829101No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26030.14132008048132436No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA25750.1397999259467577No Hit
GCCCTGGCTTCTGCAGGTACCAATTCAAATAGGTGTATCCATTACTATGC25190.1367596168776243No Hit
GGATACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTTAT24970.1355652097433219No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24690.13404505520875523No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24540.1332306867080945No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24370.132307735740679No Hit
GGACTGAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT24290.13187340587365995No Hit
GGCCAAAAGTGTAGATAAGAGTTTCTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTG24250.13165624094015044No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24240.13160194970677302No Hit
CTCCTGCATAGTAATGGATACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT24210.1314390760066409No Hit
TTATTACTGCATGCAAGCTCTAGAAACTCTTATCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA24190.13133049353988613No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23820.12932171790492303No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23590.12807301953724326No Hit
GAGTTTCTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT23510.1276386896702242No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23410.1270957773364504No Hit
ATTCTACACGGGGGCGAATCTCCTTCGGTCTGGACGTCTGGGGCCAAGGGACCACGGTCGCCGTCTCCTCAGCAT23080.12530416663499677No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT23080.12530416663499677No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA23030.12503271046810988No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22890.12427263320082653No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTAGAA22710.12329539100003366No Hit
CTCCTGTGCAGCCTCTGGATTCGGTTTTAACAGTTATGGCATGGCCTGGGTCCGCCAGGCTCCAGGGAAGGGGCT22700.12324109976665629No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACGCAGTCTC22580.1225896049661277No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG22550.12242673126599554No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22530.12231814879924079No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC22530.12231814879924079No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22450.12188381893222175No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22210.12058082933116458No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22190.12047224686440983No Hit
CTAGAAACTCTTATCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22180.12041795563103243No Hit
GCATAGTAATGGATACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGC22050.11971216959712648No Hit
GTGTAGAATACCCCCTTAAAACACCCACGCTTGCACAGTAATATACGCCC21880.11878921862971098No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT21800.11835488876269194No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21560.11705189916163478No Hit
GGTGTATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGG21370.11602036572746452No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC21200.11509741476004903No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT21140.11477166735978474No Hit
GTGTAGATAAGAGTTTCTAGAGCTTGCATGCAGTAATAAACCCCAACATC20730.1125457267913121No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20630.11200281445753828No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20610.11189423199078352No Hit
GGGATATTGTGATGACGCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT20370.11059124238972635No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20200.10966829142231088No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19740.10717089468695132No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19710.10700802098681918No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACACCTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG19620.10651939988642273No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACGCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG19600.10641081741966797No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19500.10586790508589417No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19190.10418487685119532No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTTATCGGCCACCCGGGGTCCCT19010.10320763465040245No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18960.10293617848351555No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18930.1027733047833834No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18760.10185035381596792No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18540.10065594668166551No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18510.10049307298153339No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)