FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871428
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATAATCAGCCTCATCCTCAGACTGGAGTCCACGGATGACCAGGAAAC67410.773557884300252No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC64330.7382135988285894No Hit
GATTATCACTGTGCAACTTGGGAAGACACCCTGAACGGTCCCTCTGTGGT61320.7036725925721918No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGAGGGACCGTTCAGGGTGTCTTCC45840.5260331318250044No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38310.4396232390972059No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCC35600.4085248580490872No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33710.3868363192369306No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30610.3512625254180495No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCT29450.3379510412793713No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28970.3324428409461252No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC28850.3310657908628137No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27210.3122461063908894No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCC26170.3003116723355228No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25930.2975575721688998No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25140.28849199245376556No Hit
GTCCCTCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT25000.2868854340232354No Hit
CCACAGAGGGACCGTTCAGGGTGTCTTCCCAAGTTGCACAGTGATAATCA24860.2852788755927053No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24620.28252477542608223No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23780.2728854248429015No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23280.2671477161624368No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22830.2619837783500186No Hit
GTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC22730.26083623661392563No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG22450.2576231197528654No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT20950.2404099937114713No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20790.23857392693372256No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20600.236393597635146No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20510.23536081007266232No Hit
GCTGTAAACTGGTATCAGCACCTCCCAGGAACGGCCCCCAAACTCCTCAT20270.2326067099060393No Hit
TCCGTGGACTCCAGTCTGAGGATGAGGCTGATTATCACTGTGCAACTTGGGAAGACACCCTGAACGGTCCCT19880.2281312971352768No Hit
GTTTACAGCAGTCCTTCCGATGTTGGAGTAGGTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA19830.22755752626723033No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19790.22709850957279318No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19260.22101653837150056No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA19070.21883620907292398No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18850.2163116172535195No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18730.21493456717020798No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18720.2148198129965987No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17840.2047214457189808No Hit
CAGAGGGACCGTTCAGGGTGTCTTCCCAAGTTGCACAGTGATAATCAGCC17400.19967226208017186No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGAGGGACCG17230.19772144112881387No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC17130.1965738993927209No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16690.19152471575391197No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16630.19083619071225622No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15810.1814263484762941No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT15750.18073782343463832No Hit
CTCTTGTTCTGGAACCTACTCCAACATCGGAAGGACTGCTGTAAACTGGTATCAGCACCTCCCAGGAACGGCCCC15610.1791312650041082No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15530.17821323161523386No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15200.17442634388612713No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGTTTCCTGGTCATCCGTGGACTCCAGT14900.1709837186778483No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14650.16811486433761597No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGAGGGACCGT14630.1678853559903974No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA14490.16627879755986727No Hit
CCCCTGAGGGCCGGTGATTATTATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTTTA14350.16467223912933715No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14260.16363945156685347No Hit
GTTCAGGGTGTCTTCCCAAGTTGCACAGTGATAATCAGCCTCATCCTCAG14220.16318043487241632No Hit
CCTCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCC13920.15973780966413748No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC13890.1593935471433096No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13840.15881977627526314No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC13780.15813125123360736No Hit
GTCTGGCACCTCAGGTTTCCTGGTCATCCGTGGACTCCAGTCTGAGGATG13690.15709846367112373No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC13450.15434436350450068No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAATAATAATCACCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG13400.15377059263645418No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTTTACAGCAGTCCTT13360.153311575942017No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12820.14711485056711512No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12800.14688534221989655No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12590.14447550457410135No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12570.14424599622688278No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12500.1434427170116177No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12440.14275419196996195No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGAGGGAC11990.1375902541575437No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11950.13713123746310651No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGTTTCCTGGT11930.13690172911588794No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11800.13540992485896713No Hit
GCTTGGTCCCTCCGCCGAACACCACAGAGGGACCGTTCAGGGTGTCTTCCCAAGTTGCACAGTGATAATCAGCCT11640.1335738580812184No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11600.13311484138678123No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11540.13242631634512547No Hit
TCCTCAGACTGGAGTCCACGGATGACCAGGAAACCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC11340.1301312328729396No Hit
GTTCCTGGGAGGTGCTGATACCAGTTTACAGCAGTCCTTCCGATGTTGGA11280.12944270783128384No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11170.12818041192158158No Hit
GTCTGAGGATGAGGCTGATTATCACTGTGCAACTTGGGAAGACACCCTGA11160.1280656577479723No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11040.12668860766466078No Hit
GTCCACGGATGACCAGGAAACCTGAGGTGCCAGACTTGGAGCCAGAGAAT11020.12645909931744218No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10950.1256558201021771No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG10830.1242787700188656No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10780.12370499915081912No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT10660.1223279490675076No Hit
TTCCTGGGAGGTGCTGATACCAGTTTACAGCAGTCCTTCCGATGTTGGAGTAGGTTCCAGAACAAGAGATGGTGA10650.1222131948938983No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAG10620.12186893237307042No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10580.12140991567863324No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10470.120147619768931No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10400.11934434055366594No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC10210.11716401125508935No Hit
GGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG10100.11590171534538712No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10040.11521319030373134No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG9930.11395089439402911No Hit
AGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCCCCGGGCAGAGG9870.11326236935237334No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG9840.11291810683154546No Hit
GTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG9790.11234433596349899No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9640.11062302335935958No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGGGA9530.10936072744965734No Hit
GACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGTTTCCTGGTCATCCGTGGACTCCAGTCTGAGGATGAGG9450.108442694060783No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA9440.1083279398871737No Hit
CCTCAGGTTTCCTGGTCATCCGTGGACTCCAGTCTGAGGATGAGGCTGATTATCACTGTGCAACTTGGGAAGACA9390.10775416901912722No Hit
CCCTGAACGGTCCCTCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCT9380.10763941484551794No Hit
CTCCAGTCTGAGGATGAGGCTGATTATCACTGTGCAACTTGGGAAGACAC9370.10752466067190863No Hit
GTCAGGGACCCCTGAGGGCCGGTGATTATTATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGATA9300.10672138145664357No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9270.1063771189358157No Hit
GAACACCACAGAGGGACCGTTCAGGGTGTCTTCCCAAGTTGCACAGTGAT9230.10591810224137851No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9220.10580334806776923No Hit
CAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGTTTC9010.10339351042197405No Hit
GGATGAGGCTGATTATCACTGTGCAACTTGGGAAGACACCCTGAACGGTCCCTCTGTGGTGTTCGGCGGAGGGAC8980.10304924790114617No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8940.10259023120670899No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8930.1024754770330997No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC8830.10132793529700675No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8820.10121318112339746No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8810.10109842694978816No Hit
GTATATATAGTGGTGGTGATATATTTTATGCAGATTCCGTCAAGGGCCGA8800.10098367277617887No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8780.10075416442896028No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC8750.1004099019081324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG151.7107825E-4133.4599670
GACGTTA150.001010379385.3998569
ACGTAAA150.002106019370.97430462
AGTACTA252.4234926E-455.8457450
CACGTAA200.00663130253.12956661
TAAGCGC200.007137266552.150947
CGAGTGT200.007597035751.3339915
ACGACAG306.1311293E-446.27005445
TCGTATG1300.045.1987840
TCTCGTA1300.045.0812238
GCCAGAG24450.043.94080470
CGTCTTC1350.043.84234248
GCTTAGA405.2307674E-542.797971
TATACAC2400.042.7783243
GTATGCC1550.042.44228742
CGTATGC1400.042.01412641
CCGTCTT1250.041.72072247
ACACATC2600.040.803946
CGAGCCC2450.040.50845315
TATCTCG1700.040.4849636
Produced by FastQC (version 0.11.5)