FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004692202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871428
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATAATCAGCCTCATCCTCAGACTGGAGTCCACGGATGACCAGGAAAC62500.7172135850580885No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC60500.6942627503362298No Hit
GATTATCACTGTGCAACTTGGGAAGACACCCTGAACGGTCCCTCTGTGGT58960.6765906076003984No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGAGGGACCGTTCAGGGTGTCTTCC43130.4949347507768858No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37820.4340002845903505No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCC36780.42206585053498397No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36370.41736092941700287No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28930.33198382425168804No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28240.3240657862726467No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27520.31580348577277756No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCT27350.31385266482141955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26690.30627888936320613No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC26210.30077068902996No Hit
GTCCCTCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT26020.2985903597313834No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24950.28631166315518897No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCC24450.2805739544747242No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24120.27678706674561754No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24000.275410016662306No Hit
GTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC23940.27472149162065024No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23750.27254116232207365No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23520.2699018163290599No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG23350.2679509953777019No Hit
CCACAGAGGGACCGTTCAGGGTGTCTTCCCAAGTTGCACAGTGATAATCA21050.2415575354475642No Hit
GCTGTAAACTGGTATCAGCACCTCCCAGGAACGGCCCCCAAACTCCTCAT21000.24098376457951776No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19650.22549195114226306No Hit
GTTTACAGCAGTCCTTCCGATGTTGGAGTAGGTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA19650.22549195114226306No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19460.22331162184368644No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19250.22090178419789125No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19020.2182624382048775No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA19010.21814768403126822No Hit
TCCGTGGACTCCAGTCTGAGGATGAGGCTGATTATCACTGTGCAACTTGGGAAGACACCCTGAACGGTCCCT18980.21780342151044033No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18810.21585260055908234No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18320.2102296460522269No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18300.21000013770500833No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT18240.20931161266335258No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18100.20770505423282243No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17830.2046066915453715No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16830.1931312741844421No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC16820.19301652001083278No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16780.1925575033163956No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16410.18831159889285173No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16350.18762307385119598No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGAGGGACCG15930.18280339855960562No Hit
CTCTTGTTCTGGAACCTACTCCAACATCGGAAGGACTGCTGTAAACTGGTATCAGCACCTCCCAGGAACGGCCCC15220.17465585223334573No Hit
CAGAGGGACCGTTCAGGGTGTCTTCCCAAGTTGCACAGTGATAATCAGCC15130.17362306467086208No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15090.1731640479764249No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15070.17293453962920632No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA14770.1694919144209275No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT14620.16777060181678807No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC14550.16696732260152303No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14290.1639837140876814No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14260.16363945156685347No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14100.16180338478910478No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGTTTCCTGGTCATCCGTGGACTCCAGT14020.16088535140023041No Hit
CCCCTGAGGGCCGGTGATTATTATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTTTA13620.15629518445585866No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC13410.1538853468100635No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTTTACAGCAGTCCTT13350.1531968217684077No Hit
GTTCAGGGTGTCTTCCCAAGTTGCACAGTGATAATCAGCCTCATCCTCAG13090.15021321325456607No Hit
CCTCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCC13050.1497541965601289No Hit
GTCTGGCACCTCAGGTTTCCTGGTCATCCGTGGACTCCAGTCTGAGGATG12980.14895091734486382No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGAGGGACCGT12930.14837714647681738No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12820.14711485056711512No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12660.14527878378936643No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12610.14470501292131996No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGAGGGAC12360.14183615858108758No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC12290.14103287936582254No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAATAATAATCACCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG12170.139655829282511No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12060.13839353337280877No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGTTTCCTGGT12060.13839353337280877No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11980.1374754999839344No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11670.13391812060204628No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11340.1301312328729396No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11280.12944270783128384No Hit
GTCTGAGGATGAGGCTGATTATCACTGTGCAACTTGGGAAGACACCCTGA11130.1277213952271444No Hit
GTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG10910.12519680340773992No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10880.12485254088691206No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10880.12485254088691206No Hit
AGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCCCCGGGCAGAGG10670.12244270324111688No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10620.12186893237307042No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC10520.12072139063697748No Hit
GCTTGGTCCCTCCGCCGAACACCACAGAGGGACCGTTCAGGGTGTCTTCCCAAGTTGCACAGTGATAATCAGCCT10510.12060663646336818No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10340.11865581551201018No Hit
TCCTCAGACTGGAGTCCACGGATGACCAGGAAACCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC10260.11773778212313582No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC10260.11773778212313582No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10160.11659024038704288No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10150.11647548621343358No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10130.116245977866215No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAG9910.11372138604681052No Hit
GTCCACGGATGACCAGGAAACCTGAGGTGCCAGACTTGGAGCCAGAGAAT9900.11360663187320123No Hit
GTTCCTGGGAGGTGCTGATACCAGTTTACAGCAGTCCTTCCGATGTTGGA9870.11326236935237334No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9760.11200007344267111No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC9710.11142630257462464No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC9710.11142630257462464No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9600.1101640066649224No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9570.10981974414409452No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9510.10913121910243875No Hit
TTCCTGGGAGGTGCTGATACCAGTTTACAGCAGTCCTTCCGATGTTGGAGTAGGTTCCAGAACAAGAGATGGTGA9470.10867220240800157No Hit
CCCTGAACGGTCCCTCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCT9400.10786892319273653No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT9400.10786892319273653No Hit
CTCCAGTCTGAGGATGAGGCTGATTATCACTGTGCAACTTGGGAAGACAC9390.10775416901912722No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA9290.10660662728303429No Hit
GACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGTTTCCTGGTCATCCGTGGACTCCAGTCTGAGGATGAGG9230.10591810224137851No Hit
GGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG9030.10362301876919264No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCCCCCTCAGCGTCTGGGA9020.10350826459558334No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8990.10316400207475546No Hit
GGGTCACCATCTCTTGTTCTGGAACCTACTCCAACATCGGAAGGACTGCT8880.10190170616505322No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8860.10167219781783464No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8850.10155744364422534No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT8770.10063941025535098No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8770.10063941025535098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)