FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866353
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG37270.4301941587320642No Hit
ATATTATAGTGTTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT34720.4007604290629801No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAACTCTGTC25590.29537613420857317No Hit
CTTTTATACAGCTCCAATAGTAAGAACTACTTAGCTTGGTACCAGCAGAA25550.29491442864513656No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24960.2881042715844465No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23160.2673275212297989No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21330.24620449170257389No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG20690.2388172026875881No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19930.23004479698229244No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19670.22704371081995445No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19190.2215032440587151No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17720.20453556460241956No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16910.19518602694282816No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15800.18237369755746213No Hit
CACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCA15420.17798749470481431No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15150.1748709821516172No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGG14680.16944594178123698No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14250.1644826069742934No Hit
CCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTG14180.1636746222382793No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14110.16286663750226524No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13250.15293996788837808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13230.15270911510665974No Hit
GAGTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTGTT13190.15224740954322313No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12830.14809205947229362No Hit
CGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGG12640.14589895804596972No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC12600.14543725248253309No Hit
GGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGA12560.14497554691909648No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA12270.14162818158418106No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT11910.13747283151325154No Hit
GGCCGGTACTTCGCCCACAGCATCCTGACCGTGTCCGAAGAGGAATGGAA11750.13562600925950508No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11730.13539515647778677No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11700.1350488773052093No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11470.13239407031544878No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11460.13227864392458963No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGAAGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA10770.12431422295530806No Hit
GTTTATTACTGTCAGCAATATTATAGTGTTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA10650.12292910626499821No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10590.12223654791984329No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT10580.12212112152898413No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10510.12131313679297007No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10500.12119771040211091No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10110.11669608115860393No Hit
GGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGACACGTTGTA10060.11611894920430818No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG9750.11254073108767441No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9580.11057848244306882No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9560.11034762966135052No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9510.10977049770705474No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTATTGGAGCTGTATAAAAGACT9480.10942421853447727No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9450.10907793936189983No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9340.10780824906244915No Hit
GACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCG9230.10653855876299845No Hit
GTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTG9080.10480716290011116No Hit
GTGTCGGACATGACCAGGGACACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACC8950.10330661981894217No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8790.10145979756519571No Hit
CCGAGAGGACCGTGGACAAGTCCACCGGTAAACCCACCCTGTACAACGTG8780.10134437117433656No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8710.1005363864383225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT350.00625467653.9170770
GTGGTAT2550.052.009891
CGAATCT750.050.27086334
GGTATCA14800.049.4012871
GGCGAAT850.048.31035632
TCGTATG800.047.53834540
TGGTATC3000.046.464772
ATGCCGT851.8189894E-1244.9981744
GCGAATC851.8189894E-1244.31538833
GTATCAA41650.041.313981
GCCGTCT951.9463187E-1036.7162546
CGTATGC1051.6370905E-1136.27718441
TATCAAC49050.035.003672
CCGTCTT1003.3287506E-1034.9259447
ATCAACG50350.034.1674273
TAAGGCG1201.8189894E-1234.1207229
TCAACGC50850.033.7646034
TGCCGTC1154.1836756E-1133.30473345
CAACGCA51850.033.1134035
ATCTCGT1154.9112714E-1132.9040637
Produced by FastQC (version 0.11.5)