FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190446
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGATGACAGTCTGAGTGGTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTC95150.7992802697476409No Hit
GTAATAATCAGCGTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGG84680.7113300393297974No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT48820.41009840009542636No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA43020.3613771645248923No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC42160.35415298131960626No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37400.3141679672996507No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37330.31357995238759256No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC35830.3009796328434889No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34520.28997535377497174No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34240.2876232941267391No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGCGACCC31720.26645475729264495No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31210.2621706486476497No Hit
GTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC29300.24612624176149106No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGACCACTC27800.23352592221738744No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27360.22982982848445038No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26650.22386567723357464No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26590.2233616644518105No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT26590.2233616644518105No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26020.21857354302505114No Hit
CATAAGGACCACTCAGACTGTCATCCCATGTTGCACAGTAATAATCAGCG24840.20866129165035627No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA24200.20328515531153868No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG23890.20068108927242395No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG23690.19900104666654345No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGACCACTCA23300.19572496358507652No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGACCA21620.18161260569568044No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC21240.1784205247445075No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20900.17556445231451068No Hit
TTCCTATAGATGAGGAGTTTGGGGGCCATTCCTGGGAGTTGCTGGTACCA20770.17447242462068838No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20310.17060832662716327No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20290.17044032236657522No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19410.16304813490070108No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19410.16304813490070108No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGCGACCCCCGGGCAGAGGGTCAC19160.16094808164335048No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19030.15985605394952815No Hit
CTATAGGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC18610.15632796447717914No Hit
CCCCTGAGGGCCGCTGATCATTCCTATAGATGAGGAGTTTGGGGGCCATTCCTGGGAGTTGCTGGTACCAATATA18560.15590795382570902No Hit
CCCTCAGCGTCTGCGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG18420.15473192400159266No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17850.1499438025748333No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17440.1464997152327783No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16750.14070356824249064No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT16520.13877151924572806No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16360.13742748516102368No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16060.13490742125220295No Hit
GGATGACAGTCTGAGTGGTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAA16040.1347394169916149No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT16000.1344034084704388No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC15400.12936328065279737No Hit
GGCCATCAGTGGGCTCCGGTCCGAAGATGACGCTGATTATTACTGTGCAACATGGGATGACAGTCTGAGTGGTC15250.128103248698387No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG15040.12633920396221246No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15010.1260871975713304No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14950.12558318478956626No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14870.12491116774721407No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14720.1236511357928037No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14710.12356713366250968No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC14310.1202070484507487No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAAGATG14280.11995504205986664No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14200.11928302501751445No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13880.11659495684810565No Hit
GTGCAACATGGGATGACAGTCTGAGTGGTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGC13610.11432689933016701No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC13580.11407489293928494No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13520.11357088015752079No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT13290.11163883116075825No Hit
CAGTCTGAGTGGTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA13190.110798809857818No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13140.11037879920634787No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13120.11021079494575982No Hit
CTCTTGTTCTGGAAGCACCTCCAACATCGGAACTAATTATGTATATTGGTACCAGCAACTCCCAGGAATGGCCCC12710.10676670760370482No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12410.1042466436948841No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG12030.10105456274371119No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC11930.10021454144077094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG150.00241891468.5397136
TCGTGCG351.7093074E-551.71794563
ATCGCGT200.00751343451.47900437
AGGGGGG2850.050.7040770
AACGTTG400.00893814649.26361570
GCCAGAG24950.045.0184570
GGTATCA13700.044.125391
CGTATGC1850.042.9701841
TATGCCG2100.042.90969543
ACCGATC2950.041.4778827
ACGAGAC3050.041.18902222
GTATCAA37200.039.9871331
CACGAGA2900.039.80375721
CCGATCA3000.039.6753128
TCTCGTA1850.039.01341238
CTTATAC3050.038.9724121
TCTAGAC350.001458560338.80033
AGACCGA3100.038.35188725
TCTCCGA3100.038.32772411
TCGTATG1900.038.13431540
Produced by FastQC (version 0.11.5)