FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190446
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGATGACAGTCTGAGTGGTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTC86470.7263664206524277No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC46220.3882578462189801No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43650.3666692987334159No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40700.3418886702966787No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGCGACCC37650.3162680205570013No Hit
GTAATAATCAGCGTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGG37270.3130759396058284No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34990.2939234538987909No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA34840.2926634219443805No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34650.291067381468794No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32870.27611500227645774No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC32560.27351093623734296No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32260.27099087232852226No Hit
GTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC30950.25998659326000506No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT30320.2546944590514816No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27170.22823378800886387No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26880.22579772623033723No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA26830.22537771557886707No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26190.2200015792400495No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25120.21101335129858892No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24760.20798927460800404No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG24570.2063932341324176No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC24340.20446118513565503No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGCGACCCCCGGGCAGAGGGTCAC23020.19337290393684384No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGACCACTC22580.18967681020390678No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21650.18186461208656252No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21370.17951255243832984No Hit
CTATAGGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC20560.17270837988451387No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20490.1721203649724557No Hit
CCCTCAGCGTCTGCGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG20170.16943229680304692No Hit
TTCCTATAGATGAGGAGTTTGGGGGCCATTCCTGGGAGTTGCTGGTACCA20120.16901228615157682No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA20070.16859227550010666No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19740.1658202052004039No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGACCA19120.16061207312217438No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18650.15666397299835524No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT18470.15515193465306282No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18410.15464792187129867No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18350.15414390908953451No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18280.15355589417747634No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGACCACTCA17970.15095182813836158No Hit
CCCCTGAGGGCCGCTGATCATTCCTATAGATGAGGAGTTTGGGGGCCATTCCTGGGAGTTGCTGGTACCAATATA17850.1499438025748333No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC17510.14708773014483648No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16860.1416275916757249No Hit
CATAAGGACCACTCAGACTGTCATCCCATGTTGCACAGTAATAATCAGCG16500.13860351498514004No Hit
CTCTTGTTCTGGAAGCACCTCCAACATCGGAACTAATTATGTATATTGGTACCAGCAACTCCCAGGAATGGCCCC16480.13843551072455199No Hit
GGATGACAGTCTGAGTGGTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAA15880.13339538290691053No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT15570.13079131686779577No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC15270.12827125295897504No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAAGATG15050.1264232060925065No Hit
GGCCATCAGTGGGCTCCGGTCCGAAGATGACGCTGATTATTACTGTGCAACATGGGATGACAGTCTGAGTGGTC14880.1249951698775081No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14870.12491116774721407No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14700.12348313153221567No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14310.1202070484507487No Hit
GTGCAACATGGGATGACAGTCTGAGTGGTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGC14250.11970303566898456No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14200.11928302501751445No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14160.11894701649633835No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14030.11785498880251602No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13950.11718297176016383No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13900.11676296110869372No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13690.11499891637251922No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13670.11483091211193115No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG13650.11466290785134312No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13340.11205884181222836No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC12750.10710271612488093No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12740.10701871399458691No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12270.10307061387076777No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC12100.10164257765576934No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG11960.10046654783165301No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG11960.10046654783165301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGT252.798962E-454.2425318
TAGCGTA252.7995434E-454.2402519
AGCGTAT306.884096E-445.19640720
GCGTATA306.884096E-445.19640721
GGTATCA15650.043.772681
CGGTAAT350.001466454538.75771
GTATCAA38450.038.01431
GCCAGAG17850.035.3644870
TATCAAC41900.034.389942
TTAACGG400.002697306534.23266635
TGGTATC3950.033.475262
ATCAACG43900.032.734953
TCAACGC44750.032.1131744
CAACGCA45100.032.0156025
CGGGATT652.6164913E-531.29376817
AACGCAG46300.031.1845156
TAACGGC450.004753344730.49247736
GTACCGC450.00504198930.1271387
CGCAGAG48500.029.8398448
ACGCAGA49800.028.9928347
Produced by FastQC (version 0.11.5)