FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004692207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876702
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT23930.27295477824848124No Hit
CCCCTGTGCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT20840.2377090505097513No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC20570.23462932672675552No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20390.23257617753809162No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG18960.21626504787259523No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18370.2095352810875303No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18320.208964961868457No Hit
CCTATGAGCTTACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA18250.2081665149617544No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17840.20348989736535336No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17570.20041017358235752No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17300.19733044979936168No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17050.1944788537039952No Hit
GCACAGGGGACTGGCCTGGCTTCTGCTGATACCAGAAAGCAAATTTACTC16590.18923191688852084No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16430.18740689538748628No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACTGCTGCTGTCCCACGTCTGACAGTAATAGTCAG16320.18615219310552503No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAAAATTCAGACTCAGGGAAA15940.18181776704056796No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15800.1802208732271627No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGACGTGGGACAGCAGCAGTTATGTCTTCGGAACTGGGACCAAGGT14920.17018325497147263No Hit
GACTATTACTGTCAGACGTGGGACAGCAGCAGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG14870.16961293575239933No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14820.16904261653332603No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13900.1585487429023773No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC13820.15763623215186004No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13700.15626746602608413No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA13670.15592527449464014No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13630.1554690191193815No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGAATCACCTGCTCTGG13570.15478463605649354No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13430.15318774224308832No Hit
CCCCAGGACAGACAGCCAGAATCACCTGCTCTGGAGATAGATTGGGGAGT13010.1483970608028726No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG12470.14223761323688094No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG12260.1398422725167731No Hit
CTATTACTGTCAGACGTGGGACAGCAGCAGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC12200.13915788945388513No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACTGCTGCTGTCCCACGTCTGACAGTAATAGTCAGC12130.1383594425471825No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12030.13721880410903592No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACTGCTG11960.13642035720233328No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG11870.13539378260800136No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11810.1347093995451134No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGA11300.12889214351056574No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11060.1261546112590139No Hit
GGATCCATGGCCTCCTATGAGCTTACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGAATCAC10620.12113580213116885No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT10600.12090767444353955No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT10570.12056548291209555No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10430.11896858909869032No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10420.11885452525487565No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTAT10410.118740461411061No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT10240.11680137606621178No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10060.1147482268775479No Hit
GGCCAGTCCCCTGTGCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGG10050.11463416303373324No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10000.11406384381465993No Hit
GTGCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCG9940.11337946075177198No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9780.11155443925073742No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9670.11029973696877617No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9550.10893097084300025No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCATGG9380.10699188549815103No Hit
AGCCAGGCCAGTCCCCTGTGCTGGTCATCTATCAAGATACCAAGCGGCCC9290.10596531090381908No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9180.10471060862185783No Hit
GTCTCTGGTGACTCCATCAGTACTTATTACTGGAGTTGGATCCGGCAGCC8790.10026211871308609No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8780.10014805486927142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)