FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876702
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC22440.25595926552009696No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21730.24786073260925603No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT21440.2445528811386309No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19870.22664485765972933No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19830.22618860228447063No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC19290.220029154718479No Hit
CCCCTGTGCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT18720.2135275156210434No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18360.20942121724371565No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG17090.19493510907925385No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16690.19037255532666744No Hit
CCTATGAGCTTACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA16440.18752095923130094No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16060.18318653316634387No Hit
GCACAGGGGACTGGCCTGGCTTCTGCTGATACCAGAAAGCAAATTTACTC15920.18158963935293862No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15870.18101932013386532No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15850.180791192446236No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACTGCTGCTGTCCCACGTCTGACAGTAATAGTCAG15500.1767989579127229No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15130.1725785956915805No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAAAATTCAGACTCAGGGAAA14890.16984106344002864No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14600.16653321196940352No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG14480.1651644458436276No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14060.1603737644034119No Hit
GACTATTACTGTCAGACGTGGGACAGCAGCAGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG13930.1588909344338213No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13750.1568377852451574No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGACGTGGGACAGCAGCAGTTATGTCTTCGGAACTGGGACCAAGGT13600.15512682758793753No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGAATCACCTGCTCTGG13480.1537580614621616No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA13210.15067833767916577No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12780.1457735923951354No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC12490.14246574092451025No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG12350.14086884711110503No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACTGCTG12230.13950008098532912No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC12180.13892976176625582No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12040.13733286795285057No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG11680.1332265695755228No Hit
CCCCAGGACAGACAGCCAGAATCACCTGCTCTGGAGATAGATTGGGGAGT11630.1326562503564495No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11440.13048903732397096No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACTGCTGCTGTCCCACGTCTGACAGTAATAGTCAGC11320.12912027119819505No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT11270.12854995197912175No Hit
GGATCCATGGCCTCCTATGAGCTTACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGAATCAC11210.1278655689162338No Hit
CTATTACTGTCAGACGTGGGACAGCAGCAGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC10750.12261863210075943No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10610.12102173828735419No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGA10530.12010922753683691No Hit
GTGCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCG10390.11851233372343169No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT10240.11680137606621178No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA10170.11600292915950915No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10150.11577480147187984No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG9900.11292320537651333No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9620.10972941774970285No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9610.10961535390588821No Hit
GGCCAGTCCCCTGTGCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGG9580.10927316237444422No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTAT9540.10881690699918557No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG9510.10847471546774161No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT9450.10779033240485365No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8930.10185901252649132No Hit
AGCCAGGCCAGTCCCCTGTGCTGGTCATCTATCAAGATACCAAGCGGCCC8790.10026211871308609No Hit
GTCTCTGGTGACTCCATCAGTACTTATTACTGGAGTTGGATCCGGCAGCC8790.10026211871308609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)