FastQCFastQC Report
Wed 8 May 2024
EGAF00004692209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207721
Total Bases88.8 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAACTTGGTCCCTCCGCCAAATAGCAAATTATTATTT52070.43114262317207364No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAATAGCAA47170.3905703386792148No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47010.38924552938965207No Hit
GTAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG45880.37988906378211523No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC45070.37318221675370383No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC44110.3652333610163274No Hit
GACTATTACTGCTCCTCATATGCTGGAAATAATAATTTGCTATTTGGCGG43220.35786410934313473No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40480.3351767502593728No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT39570.3276418974249848No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35440.29344525763814655No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA35220.2916236448649978No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTACTTATGACTTTGTCTCCT34210.28326078622463297No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC31600.26164983468864084No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29900.24757373598703675No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29360.2431025046347625No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT28530.2362300564451558No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA28020.23200722683467456No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27930.23126202160929551No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTACTTATG27640.22886080477196305No Hit
CATTGGTACTTATGACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAG26750.22149155309877036No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26700.22107755019578199No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG26090.21602671477932403No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25360.20998227239569403No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA24810.20542824046282213No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA24710.2046002346568454No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT24510.20294422304489196No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACTGCTCCTCATAT23990.19863859285381308No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG23720.19640297717767596No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGT23610.1954921707911016No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT23190.1920145464059994No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23080.191103740019425No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA23020.190606936535839No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT22980.1902757342134483No Hit
CTATTACTGCTCCTCATATGCTGGAAATAATAATTTGCTATTTGGCGGAG22960.19011013305225297No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCAAATAGCAAATTATTATTTC22930.18986173131045997No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT22820.18895092492388557No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22420.1856389016999787No Hit
CCCCTGAGGGTCGCTTAGTGACCTCAGACATTATGAGCTTGGGGGCTTTG22250.18423129182981832No Hit
GTCAGGGAGGCCGTGTGGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC21580.17868365292977434No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTGGCCAGACTTGGAGCCAGAGAAG21110.1747920256416838No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGG20990.17379841867451176No Hit
GACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGTCGCTTAGTGAC20820.17239080880435134No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC20480.16957558906403053No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20340.16841638093566313No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG19940.16510435771175627No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19810.16402795016398655No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19310.159887921134103No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT19200.15897711474752863No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19080.15798350778035655No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18750.15525108862063342No Hit
ACCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCCACACGG18510.1532638746862893No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCCACACGGCC18100.1498690508817848No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTGGCC18070.14962064913999176No Hit
GGATGAGGCTGACTATTACTGCTCCTCATATGCTGGAAATAATAATTTGC17770.14713663172206162No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC17710.1466398282384756No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC17610.1458118224324989No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA17530.14514941778771753No Hit
CAGTAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGA17160.1420857963056037No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT17150.14200299572500602No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17010.14084378759663863No Hit
ACTTATGACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAA16920.1400985823712596No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT16590.13736616321153644No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA16530.13686935972795042No Hit
GTACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTCC16210.13421974114882493No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16210.13421974114882493No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA16190.1340541399876296No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG16110.13339173534284823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.13165292315029714No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA15880.1314873219891018No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15530.1285893016681833No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15500.12834089992639028No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACT15300.12668488831443686No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15160.12552568018606947No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT15130.12527727844427644No Hit
CAACAACACCCAGGCAAAGCCCCCAAGCTCATAATGTCTGAGGTCACTAA14970.12395246915471371No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA14900.12337286509053No Hit
CTCCAAGTCTGGCCACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTG14890.12329006450993235No Hit
CATTATGAGCTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAA14860.12304166276813933No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC14850.12295886218754166No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC14780.12237925812335795No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC14400.11923283606064645No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT14400.11923283606064645No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14280.1182392290934744No Hit
CCTCATATGCTGGAAATAATAATTTGCTATTTGGCGGAGGGACCAAGTTG14270.11815642851287673No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14180.11741122328749769No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14100.11674881864271631No Hit
TCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTGGCCAGACTT14010.11600361341733728No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA13990.11583801225614195No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13930.11534120877255592No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13780.11409920006359084No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG13750.11385079832179784No Hit
GCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTGGCCAGACTTGGAGCC13750.11385079832179784No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG13710.11351959599940716No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACTGC13700.11343679541880947No Hit
CAGGGAGGCCGTGTGGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCC13590.1125259890322351No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13510.11186358438745372No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT13430.11120117974267237No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA13290.11004197161430497No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTC13200.10929676638892591No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTACTTATGACTT13150.10888276348593756No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12970.1073923530351795No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12790.10590194258442141No Hit
GCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTA12750.10557074026203073No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC12720.1053223385202377No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12630.10457713329485867No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC12610.10441153213366333No Hit
GCATATGAGGAGCAGTAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAGA12590.10424593097246798No Hit
CCTCAGACATTATGAGCTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAG12580.1041631303918703No Hit
CCTGAGGGTCGCTTAGTGACCTCAGACATTATGAGCTTGGGGGCTTTGCC12550.1039147286500773No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG12540.10383192806947962No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12530.10374912748888196No Hit
GTGTTGTTGGTACCAGGAGACAAAGTCATAAGTACCAATGTCACTGCTGG12430.10292112168290525No Hit
ACTTGGTCCCTCCGCCAAATAGCAAATTATTATTTCCAGCATATGAGGAG12310.10192751471573319No Hit
GGCAAAGCCCCCAAGCTCATAATGTCTGAGGTCACTAAGCGACCCTCAGG12280.10167911297394017No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC12110.10027150310377977No Hit
CATAATGTCTGAGGTCACTAAGCGACCCTCAGGGGTCCCTGATCGCTTCT12110.10027150310377977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)