FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207721
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAATAGCAAATTATTATTTCCAGCATATGAGGAG46240.38286988468363137No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCAAATAGCAAATTATTATTTCCAGCATATGAGGAGCAGTAATAGT45510.37682544230000137No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42430.3513228634759187No Hit
GACTATTACTGCTCCTCATATGCTGGAAATAATAATTTGCTATTTGGCGGAGGGACCAAGTTGACCGTCCTAGGT39830.32979471252052417No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG39180.32441267478167557No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38690.3203554463323897No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37040.30669335053377395No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT36050.29849609305460445No Hit
GTAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG35160.29112684138141176No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34670.2870696129321259No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTACTTATGACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC31260.25883461494832No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC29880.24740813482584137No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28850.23887967502428126No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28710.2377204668959139No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26270.21751712523008213No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26090.21602671477932403No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25080.20766385613895924No Hit
CATTGGTACTTATGACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAGCTCATAATGTCTGAGGT24810.20542824046282213No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24800.20534543988222445No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTACTTATGACTTTGTCTCCTGGTACCAACAACA24310.20128821143293854No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC24220.20054300620755955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23490.19449856382392955No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22970.19019293363285064No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22950.1900273324716553No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22710.1880401185373112No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22160.18348608660443927No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACTGCTCCTCATAT22110.18307208370145092No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21390.1771104418984186No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21390.1771104418984186No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21370.17694484073722325No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20790.17214240706255834No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20570.17032079428940955No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19810.16402795016398655No Hit
CCCCTGAGGGTCGCTTAGTGACCTCAGACATTATGAGCTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA19320.15997072171470067No Hit
CTATTACTGCTCCTCATATGCTGGAAATAATAATTTGCTATTTGGCGGAG19280.15963951939230997No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18850.1560790944266101No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTGGCCAGACTTGGAGCCAGAGAAG18750.15525108862063342No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18710.15491988629824271No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18700.15483708571764507No Hit
GACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGTCGCTTAGTGAC18690.15475428513704736No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18380.15218746713851958No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCAAATAGCAAATTATTATTTC17910.14829583985042902No Hit
GTCAGGGAGGCCGTGTGGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC17840.14771623578624535No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17770.14713663172206162No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC17640.14606022417429193No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT17140.14192019514440837No Hit
GGATGAGGCTGACTATTACTGCTCCTCATATGCTGGAAATAATAATTTGCTATTTGGCGGAGGGACCAAGTTGAC16960.14042978469365028No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16360.13546174985779003No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT16340.13529614869659468No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC16290.1348821457936063No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15930.13190132489209014No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15910.1317357237308948No Hit
ACTTATGACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAGCTCATAATGTCTGAGGTCACTAAG15790.13074211676372274No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15700.12999691153834372No Hit
ACCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCCACACGG15580.12900330457117165No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15540.12867210224878095No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTA15520.12850650108758563No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCCACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG15270.12643648657264384No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC15230.12610528425025316No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15060.12469767438009274No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA14960.12386966857411603No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14810.12262765986515098No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14790.12246205870395563No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT14710.12179965405917427No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14570.12064044593080687No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14540.12039204418901385No Hit
GTACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG14390.1191500354800488No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACT14110.116831619223314No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT14000.1159208128367396No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13690.1133539948382118No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13500.11178078380685605No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTC13350.11053877509789099No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTGGCC13280.10995917103370728No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13190.10921396580832825No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13190.10921396580832825No Hit
CAGTAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGA13100.1084687605829492No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13040.10797195709936318No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12870.10656434722920277No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12850.10639874606800744No Hit
CAACAACACCCAGGCAAAGCCCCCAAGCTCATAATGTCTGAGGTCACTAAGCGACCCTCAGGGGTCCCTGATCG12820.10615034432621441No Hit
CTCCAAGTCTGGCCACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACTGCTC12720.1053223385202377No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACTGC12710.10523953793964004No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12580.1041631303918703No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12510.10358352632768661No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12390.10258991936051456No Hit
CATTATGAGCTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAAGTCATAAGTACCAATGTCACTG12250.10143071123214717No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12210.10109950890975648No Hit
CCTCATATGCTGGAAATAATAATTTGCTATTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTG12190.10093390774856115No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTACTTATGACTT12180.10085110716796346No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT12160.10068550600676812No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC12080.10002310136198675No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12080.10002310136198675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)