FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620527
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATGCTTCTCCTCGTACATTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT153080.944630974985298No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT113100.6979211083801751No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC100640.6210325406488136No Hit
GCATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC91820.5666058016929061No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC73920.4561479074399871No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC73790.4553456992694352No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTCACCGGAAATCCGGGGTCTCT72990.45040903360450024No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG66130.4080771255276833No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA63790.3936373784577486No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT61310.37833371489645035No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTTGTTCATATTGTTGGAGCTGTAAAAAAGACT59840.36926259173713244No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC56930.35130547038093163No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG55660.3434685136378474No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG53930.33279297413742565No Hit
GTTTATTACTGTCAGCAGTATTATGCTTCTCCTCGTACATTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA51930.3204513099750883No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTCACCGGAAA50880.31397193628986125No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC49710.3067520627548939No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG49320.3043454382432381No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT49270.3040368966391797No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT49020.30249418861888755No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG48530.29947048089911493No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT48440.29891510601180976No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT47950.2958913982920371No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC46620.28768419162408276No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAATGTA43690.2696036536262586No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG43050.26565432109431064No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC42990.2652840711694406No Hit
GTCTCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC41830.2581259059552849No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGTC38800.23942828474934388No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATT38660.23856436825798027No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG38350.23665141031281797No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA37540.23165303632707138No Hit
GTCAGAGACCCCGGATTTCCGGTGAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA37220.2296783700610974No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA35750.2206072469017795No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG35100.21659620604901983No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34150.21073391557190962No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATGCTTCT33870.2090060825891824No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA33120.20437795852830593No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA33100.20425454188668254No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT32770.20221816729989686No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT31090.19185116940353353No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGT30450.18790183687158563No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA29940.18475471251018957No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT29450.18173100479041696No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA29360.18117562990311176No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC29320.180928796619865No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT28880.17821363050415082No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTTGTTCATATTGTTGGAGCTG28860.17809021386252744No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGTCAGAGTCTTTTTTACAGCTCCAACAATATGAACAA28710.17716458905035215No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT28530.17605383927574178No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG28520.1759921309549301No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA28410.17531333942600155No Hit
CACCATCAGCAGTCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATGCTTCTCCTCGTACATT28280.17451113125544962No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT27950.17247475666866396No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC27870.17198109010217047No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG27710.17099375696918348No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC27640.1705617987235017No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT27630.17050009040268999No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT27020.1667358828331771No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC26810.16544000809613169No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA26360.1626631336596058No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA26230.16186092548905387No Hit
GGATCTCTGGTGCCTGCGGGGTCATCGTGATGACCCAGTCTCCCGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG25940.16007138418551495No Hit
GGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC25760.1589606344109046No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC25220.15562838508707352No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGGCTGAAGATG25060.15464105195408653No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT24820.15316005225460605No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC24820.15316005225460605No Hit
GGGTCATCGTGATGACCCAGTCTCCCGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGT24510.15124709430944377No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC23990.14803826162723607No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23660.14600188704045042No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG23510.1450762622282751No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATGCTTCTCCTC23440.14464430398259331No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT23230.1433484292455479No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23230.1433484292455479No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC23220.1432867209247362No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG23200.14316330428311286No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC22640.1397076383176584No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG22590.13939909671359996No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGTCAGAGTCTTTTTTACAGCTCCAA22430.13841176358061297No Hit
CTTTTTTACAGCTCCAACAATATGAACAACTTAGCTTGGTACCAGCAGAA22280.13748613876843768No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT22240.13723930548519092No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC22230.13717759716437924No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA22150.13668393059788575No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA22150.13668393059788575No Hit
GTCTCCCGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGTCAGAGTCTTTTTTA22100.1363753889938273No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC22060.13612855571058058No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT22000.13575830578571044No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA21670.1337219311989248No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC21220.1309450567623989No Hit
CAATATGAACAACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTCA21190.13075993179996384No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTCACCGGAAATCCGGGGTCTCTGA21170.13063651515834046No Hit
TTATTACTGTCAGCAGTATTATGCTTCTCCTCGTACATTCGGCCAAGGGA21150.1305130985167171No Hit
CCACTGAATCGGTCAGAGACCCCGGATTTCCGGTGAGATGCCCAGTAAAT20690.12767451575937952No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20580.12699572423045094No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC20580.12699572423045094No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTGCGGGGTCATCGTGATGA20400.1258849744558406No Hit
GTCATCGTGATGACCCAGTCTCCCGACTCCCTGGCTGTGTCTCTGGGCGA20350.12557643285178216No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT20290.12520618292691205No Hit
GCCTCCTAAGTTGCTCATTTACTGGGCATCTCACCGGAAATCCGGGGTCTCTGACCGATTCAGTGGCAGCGGGT19800.12218247520713942No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGGCTGAAGAT19590.12088660047009397No Hit
CCCTGGTCCCTTGGCCGAATGTACGAGGAGAAGCATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGC19560.12070147550765893No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19430.11989926733710701No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19420.11983755901629532No Hit
CCATCAGCAGTCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAG19340.11934389244980183No Hit
TCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGAGACC19100.11786289275032134No Hit
GGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG19010.11730751786301616No Hit
CAGCAGTATTATGCTTCTCCTCGTACATTCGGCCAAGGGACCAGGGTGGA18940.11687555961733435No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18630.11496260167217208No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18460.1139135602183734No Hit
GCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAATGTACGAGG18430.11372843525593836No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA18230.1124942688397046No Hit
GCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTCACCGGAAATCCGGGGTCTCTGACCGATT17880.11033447761129558No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC17850.11014935264886051No Hit
GCTCCAACAATATGAACAACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGG17820.10996422768642547No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG17770.10965568608236705No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT17610.10866835294938006No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG17570.1084215196661333No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT17530.10817468638288656No Hit
GCTCATTTACTGGGCATCTCACCGGAAATCCGGGGTCTCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT17500.10798956142045149No Hit
GTACGAGGAGAAGCATAATACTGCTGACAGTAATAAACTGCCACATCTTC17480.10786614477882811No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT17480.10786614477882811No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGC17440.10761931149558138No Hit
GTCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATGCTTCTCCTCGTACATTCGGCCAAGGGA17240.10638514507934765No Hit
CATTTACTGGGCATCTCACCGGAAATCCGGGGTCTCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA17240.10638514507934765No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA17040.1051509786631139No Hit
GTAAAAAAGACTCTGACTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCGGGAGA16860.10404022888850356No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG16530.1020038543017179No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG16530.1020038543017179No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16430.10138677109360103No Hit
TCTCTGGTGCCTGCGGGGTCATCGTGATGACCCAGTCTCCCGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCA16340.10083139620629585No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG16260.10033772963980236No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA16260.10033772963980236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)