FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620527
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATGCTTCTCCTCGTACATTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT149970.9254396872128635No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT109550.6760146544920264No Hit
GCATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC101890.6287460807502744No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC86270.53235768364242No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC75740.4673788218277141No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC75180.4639231558622596No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT64280.39666108617752127No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTCACCGGAAATCCGGGGTCTCT62540.3859238383562878No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA60540.3735821741939505No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG59930.3698179666244376No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC55360.34161726401349685No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTTGTTCATATTGTTGGAGCTGTAAAAAAGACT54040.3334717656663542No Hit
GTTTATTACTGTCAGCAGTATTATGCTTCTCCTCGTACATTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA52120.3216237680705104No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT51430.31736589393450404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51350.3168722273680105No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC50910.3141570612522963No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT50070.30897356230411466No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT49230.30379006335593295No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC48200.29743410631232925No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA47820.29508919012148516No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG47280.29175694079765413No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT46010.2839199840545699No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAATGTA45880.283117775884018No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG45430.28034090144749213No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG44200.2727507779876546No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC42710.26355623818671337No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42600.2628774466577848No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT41930.25874298916340177No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTCACCGGAAA40510.24998040760814227No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA39600.24436495041427883No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGAGACCCCGGATTTCCGGTG38680.23868778489960366No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38510.23763874344580496No Hit
GTCTCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC38440.23720678520012314No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG35990.22208824660125995No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGTC35370.2182623307109354No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA34910.2154237479535978No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG34430.21246174855463684No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGAGACCCCG33160.20462479181155266No Hit
GTCAGAGACCCCGGATTTCCGGTGAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA33100.20425454188668254No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATT32080.1979602931638905No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA31990.19740491827658535No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA31090.19185116940353353No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATGCTTCT30570.18864233672132585No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG30030.18531008739749477No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29470.18185442143204034No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA29300.18080537997824164No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA28910.17839875546658587No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT28870.1781519221833391No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT27850.1718576734605471No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTTGTTCATATTGTTGGAGCTG27810.17161084017730036No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT27370.16889567406158612No Hit
TCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGAGACC27370.16889567406158612No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC27180.1677232159661641No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT27020.1667358828331771No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC26920.16611879962506024No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC26310.16235459205554736No Hit
GGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG25850.15951600929820978No Hit
CACCATCAGCAGTCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATGCTTCTCCTCGTACATT25770.1590223427317163No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC25700.15859038448603446No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA25690.15852867616522281No Hit
GGATCTCTGGTGCCTGCGGGGTCATCGTGATGACCCAGTCTCCCGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG25620.158096717919541No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT25540.1576030513530475No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC25370.15655400989924884No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT25130.15507301019976835No Hit
GGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC25070.15470276027489824No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATGCTTCTCCTC24840.15328346889622943No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGT24830.15322176057541775No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24750.15272809400892426No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA24420.1506917194221386No Hit
GGGTCATCGTGATGACCCAGTCTCCCGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGT24270.1497660946099633No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC24250.14964267796833994No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGGCTGAAGATG24110.14877876147697633No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG24050.1484085115521062No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA23840.1471126368150608No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGTCAGAGTCTTTTTTACAGCTCCAACAATATGAACAA23780.14674238689019067No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA23720.14637213696532056No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT23300.1437803874912297No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCG22930.1414971796211973No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT22910.1413737629795739No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA22800.14069497145064538No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC22700.1400778882425285No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC22530.13902884678872984No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT22460.13859688854304802No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT22450.13853518022223635No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT22170.13680734723950913No Hit
CCCTGGTCCCTTGGCCGAATGTACGAGGAGAAGCATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGC21500.13267288974512612No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC21460.13242605646187938No Hit
TTATTACTGTCAGCAGTATTATGCTTCTCCTCGTACATTCGGCCAAGGGA21360.1318089732537625No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC20980.12946405706291841No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT20790.1282915989674964No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA20770.12816818232587301No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTGCGGGGTCATCGTGATGA20670.12755109911775614No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG20090.12397201651067832No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGTCAGAGTCTTTTTTACAGCTCCAA20050.12372518322743156No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG20010.12347834994418483No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA19930.12298468337769133No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC19700.12156539199902254No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC19660.12131855871577578No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC19580.12082489214928231No Hit
GTCTCCCGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGTCAGAGTCTTTTTTA19570.12076318382847062No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19160.11823314267519146No Hit
GTACGAGGAGAAGCATAATACTGCTGACAGTAATAAACTGCCACATCTTC18920.11675214297571099No Hit
CTTTTTTACAGCTCCAACAATATGAACAACTTAGCTTGGTACCAGCAGAA18600.11477747670973702No Hit
GCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAATGTACGAGG18490.11409868518080847No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18480.11403697685999678No Hit
GTCATCGTGATGACCCAGTCTCCCGACTCCCTGGCTGTGTCTCTGGGCGA18300.11292622708538644No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG18130.11187718563158776No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT18080.1115686440275293No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG18050.11138351906509426No Hit
CAGCAGTATTATGCTTCTCCTCGTACATTCGGCCAAGGGACCAGGGTGGA17940.1107047275361657No Hit
CCATCAGCAGTCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAG17810.10990251936561378No Hit
CCACTGAATCGGTCAGAGACCCCGGATTTCCGGTGAGATGCCCAGTAAAT17710.1092854361574969No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGC17590.10854493630775668No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17440.10761931149558138No Hit
GTCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATGCTTCTCCTCGTACATTCGGCCAAGGGA17280.10663197836259439No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA17270.1065702700417827No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT17130.10570635355041909No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC17050.1052126869839256No Hit
GGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTGCGGGGTCATCGTGATGACCCAGTCTCCCGACTCC16940.10453389545499706No Hit
ATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGA16820.1037933956052568No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGGCTGAAGAT16790.10360827064282176No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTCACCGGAAATCCGGGGTCTCTGA16720.10317631239713994No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG16690.10299118743470488No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG16640.10268264583064646No Hit
GCCTCCTAAGTTGCTCATTTACTGGGCATCTCACCGGAAATCCGGGGTCTCTGACCGATTCAGTGGCAGCGGGT16550.10212727094334127No Hit
CAATATGAACAACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTCA16350.10089310452710754No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16350.10089310452710754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)