FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900683
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36550.40580315160827946No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA35350.3924799291204564No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGTAGAGCGGTGCTGCTTGTATATGAGCTG32810.3642791081878974No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCCCCTG28800.3197573397077551No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGTAGAGCGGTGCTGCTTGTATATGAGCTGCAGTAATAAT27420.30443563384675854No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25840.2868933909044581No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25430.2823412898877852No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24750.27479146381135205No Hit
GTCGATCACCATCCCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATACCTATGTCTCCTGGTACCAACAGCA23960.26602034234020183No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22140.24581345490033674No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21970.24392599838122847No Hit
GATTATTACTGCAGCTCATATACAAGCAGCACCGCTCTACTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT21550.23926287051049036No Hit
GTGTATTACTGTGTAAGAGAATCGGAGGACGGCCGTGGCTGGTCAATTAC21320.23670925286699093No Hit
CTCCTGGACAGTCGATCACCATCCCCTGCACTGGAACCAGCAGTGACGTT20680.2296035342068186No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19290.2141708014917568No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18830.20906356620475794No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18810.20884151249662758No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18670.20728713653971487No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18600.2065099485612585No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17430.19351980663563095No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT17330.19240953809497904No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17090.1897448935974144No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG17040.18918975932708845No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16460.18275020179130727No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16450.18263917493724208No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16430.1824171212291117No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16410.1821950675209813No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16360.18163993325065533No Hit
GGTTATACCTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCGTGATTTATGAGGTCAGTAAT16300.18097377212626417No Hit
GTCTCTAATTACTCCATCAGCAGTGGTTACTTCTGGGGCTGGGTCCGGCA16200.17986350358561226No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16030.17797604706650397No Hit
ATCTATCATAGTGCGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT15910.17664372481772167No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15640.17364599975796144No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA15530.17242470436324434No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14930.16576309311933277No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14920.1656520662652676No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCC14380.15965661614574717No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14330.15910148187542122No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14230.1579912133347693No Hit
CCCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATACCTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC14080.1563258105237914No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13970.15510451512907428No Hit
ATCATAGTGCGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACC13970.15510451512907428No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13830.15355013917216156No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13670.15177370950711846No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13410.14888701130142346No Hit
ATGATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGCCGGACCCAGCCCCAGAAGTAACCACTG13320.14788776961483674No Hit
GGACTGGTGAGGCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTCTAATTACTCCATCAGCAGTGGTTACTT13240.14699955478231522No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13180.14633339365792405No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13100.1454451788254025No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12910.14333566859816385No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12900.14322464174409863No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12870.14289156118190308No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12720.14122615837092517No Hit
GTATTACTGTGTAAGAGAATCGGAGGACGGCCGTGGCTGGTCAATTACCCCCTTCTTCTACTTTGACTACTGGGG12590.13978280926807768No Hit
GTCCCTCACCTGCGCTGTCTCTAATTACTCCATCAGCAGTGGTTACTTCT12560.1394497287058821No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGTAGAGCGGTGCTGC12440.13811740645709977No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCCTATCAATAGACACGTCC12340.13700713791644786No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12080.13412043971075285No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11980.13301017117010092No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC11920.13234401004570975No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11760.13056758038066668No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11720.1301234729644059No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11720.1301234729644059No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11720.1301234729644059No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11570.128458070153428No Hit
ATCAATAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTA11520.12790293588310206No Hit
GTAATCGGCCCTCCGGAATTTCTTATCGCTTCTCTGGCTCCAAGTCTGGC11460.1272367747587109No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11420.12679266734245012No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG11410.12668164048838493No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGTAGAG11280.1252382913855374No Hit
TATACAAGCAGCACCGCTCTACTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC11190.1242390496989507No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11180.12412802284488549No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11170.1240169959908203No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10850.12046413666073413No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10740.119242841266017No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10600.1176884653091043No Hit
GCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC10600.1176884653091043No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10530.11691127733064796No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10500.1165781967684524No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10470.11624511620625681No Hit
GTGTAAGAGAATCGGAGGACGGCCGTGGCTGGTCAATTACCCCCTTCTTCTACTTTGACTACTGGGGCCAGGGAA10410.11557895508186565No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10330.11469074024934411No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10280.11413560597901816No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAGGCC10270.11402457912495295No Hit
CTTCTGGGGCTGGGTCCGGCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATCATAGTGCGAGCAC10200.1132473911464966No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10080.11191506889771428No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10040.11147096148145352No Hit
GTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTC10030.11135993462738833No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGAAGAAGGGGGTAATTGACCAGCCACGGCCGTCCTCCGATTCTCTTA10000.11102685406519275No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGTAGAGCGGTG9960.11058274664893199No Hit
GTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTG9880.10969453181641044No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCGTGATTTATGAGGTCA9860.10947247810828005No Hit
GCTTGGTCCCTCCGCCGAAAAGTAGAGCGGTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAG9820.10902837069201927No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC9700.10769604844323698No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9520.1056975650700635No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC9500.1054755113619331No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9460.10503140394567234No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG9430.10469832338347676No Hit
GGATTGGGAGTATCTATCATAGTGCGAGCACCTACTACAACCCGTCCCTC9310.10336600113469445No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT9300.10325497428062926No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9210.10225573259404251No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9190.10203367888591214No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT9180.10192265203184693No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9120.1012564909074558No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9110.1011454640533906No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9060.10059032978306462No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG9030.10025724922086904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2200.076.87308570
TATAGTG300.002546768467.6483270
TATTGAT13100.051.63993570
CTCGAAG200.007581650351.3604531
CGTATGC2500.051.1368241
TCGTATG2400.050.3316540
ACGAGAC2650.048.97116522
GAGCGAT2400.048.54222532
GGTATCA5350.048.5296631
GTCGTAT506.983646E-848.26421739
ATGCCGT2550.047.60067444
TCTCGTA2000.046.49358738
TATACAC2850.045.5295373
CCCACGA2900.044.7445419
ACACATC3000.044.3912966
ATCTCCG3000.044.38883210
TGAGCGA2800.044.02324331
CTTATAC2950.044.005711
CACGAGA2950.043.99104721
CACATCT3150.043.359067
Produced by FastQC (version 0.11.5)