FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900683
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34550.38359778079524093No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA33570.37271714909685205No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCCCCTG29600.3286394880329705No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGTAGAGCGGTGCTGCTTGTATATGAGCTG29470.32719613893012306No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27430.3045466607008237No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGTAGAGCGGTGCTGCTTGTATATGAGCTGCAGTAATAAT25210.2798986990983509No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23620.26224542930198524No Hit
GTCGATCACCATCCCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATACCTATGTCTCCTGGTACCAACAGCA22410.24881117996009694No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21990.24414805208935886No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21020.23337844724503515No Hit
CTCCTGGACAGTCGATCACCATCCCCTGCACTGGAACCAGCAGTGACGTT20900.23204612499625282No Hit
GTGTATTACTGTGTAAGAGAATCGGAGGACGGCCGTGGCTGGTCAATTAC20380.2262727285848628No Hit
GATTATTACTGCAGCTCATATACAAGCAGCACCGCTCTACTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT20000.2220537081303855No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19920.22116549329786397No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19740.21916700992469046No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19470.21616928486493028No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18650.20706508283158445No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18390.20417838462588947No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17920.1989601224848254No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT17650.19596239742506522No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17330.19240953809497904No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17180.19074413528400114No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16840.18696922224578458No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC16420.1823060943750465No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16360.18163993325065533No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16320.18119582583439456No Hit
GGTTATACCTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCGTGATTTATGAGGTCAGTAAT16060.17830912762869955No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15550.17264675807137472No Hit
GTCTCTAATTACTCCATCAGCAGTGGTTACTTCTGGGGCTGGGTCCGGCA15390.17087032840633165No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15040.1669843885140499No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG14860.1649859051408764No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14860.1649859051408764No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14770.1639866634542897No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14660.1627653680595726No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14600.16209920693518143No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14580.161877153227051No Hit
ATCTATCATAGTGCGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT14460.16054483097826872No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13990.15532656883720464No Hit
CCCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATACCTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC13980.15521554198313947No Hit
GTCCCTCACCTGCGCTGTCTCTAATTACTCCATCAGCAGTGGTTACTTCT13750.15266192433964004No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA13660.1516626826530533No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13530.1502193335502058No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13500.14988625298801023No Hit
GGACTGGTGAGGCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTCTAATTACTCCATCAGCAGTGGTTACTT13440.14922009186361906No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13240.14699955478231522No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC13110.1455562056794677No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13090.1453341519713373No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCC13060.14500107140914173No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12920.14344669545222904No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12730.14133718522499036No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12620.14011588983027326No Hit
GTATTACTGTGTAAGAGAATCGGAGGACGGCCGTGGCTGGTCAATTACCCCCTTCTTCTACTTTGACTACTGGGG12620.14011588983027326No Hit
ATGATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGCCGGACCCAGCCCCAGAAGTAACCACTG12600.13989383612214287No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12560.1394497287058821No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12350.13711816477051303No Hit
ATCATAGTGCGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACC12340.13700713791644786No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12220.13567481566766554No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12100.1343424934188832No Hit
ATCAATAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTA11860.1316778489213186No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11470.1273478016127761No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11440.1270147210505805No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11310.125571371947733No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCCTATCAATAGACACGTCC11240.12479418396927665No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG11220.12457213026114626No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11190.1242390496989507No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11070.12290672745016837No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10910.1211302977851253No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10870.12068619036886452No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGAAGAAGGGGGTAATTGACCAGCCACGGCCGTCCTCCGATTCTCTTA10850.12046413666073413No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG10850.12046413666073413No Hit
GTAATCGGCCCTCCGGAATTTCTTATCGCTTCTCTGGCTCCAAGTCTGGC10620.1179105190172347No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10570.11735538474690875No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT10540.11702230418471317No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10540.11702230418471317No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGTAGAG10430.11580100878999604No Hit
GTGTAAGAGAATCGGAGGACGGCCGTGGCTGGTCAATTACCCCCTTCTTCTACTTTGACTACTGGGGCCAGGGAA10310.11446868654121371No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10220.11346944485462698No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10150.11269225687617064No Hit
GTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTG10150.11269225687617064No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGTAGAGCGGTGCTGC10130.11247020316804024No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10060.11169301518958391No Hit
GCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC10050.1115819883355187No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9920.11013863923267121No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9860.10947247810828005No Hit
CTCTTACACAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGC9830.10913939754608447No Hit
TATACAAGCAGCACCGCTCTACTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC9820.10902837069201927No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG9750.10825118271356293No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC9700.10769604844323698No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG9660.1072519410269762No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGTAGAGCGGTG9660.1072519410269762No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9640.1070298873188458No Hit
GTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTC9620.10680783361071544No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9610.10669680675665022No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9610.10669680675665022No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9540.10591961877819388No Hit
CTTCTGGGGCTGGGTCCGGCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATCATAGTGCGAGCAC9450.10492037709160715No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9430.10469832338347676No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG9270.10292189371843367No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9250.10269984001030329No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAGGCC9250.10269984001030329No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9240.10258881315623809No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9140.10147854461558617No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9140.10147854461558617No Hit
GCTTGGTCCCTCCGCCGAAAAGTAGAGCGGTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAG9110.1011454640533906No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCGTGATTTATGAGGTCA9070.10070135663712981No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT9070.10070135663712981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)