FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594257
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTTTGGTAGTTCACCTACGTGGACGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAA58630.36775751964708325No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41950.26313197934837357No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCA39240.24613346530703648No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38250.23992367604470294No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28740.1802720640398631No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28680.17989571317547923No Hit
GTGTTAGCAGAGAGCACTTAGCCTGGTACCAACAGAAGGGAGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCAT28260.17726125712479232No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27460.17224324559967433No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27200.170612391854011No Hit
GTGTATTACTGTCAGCAGTTTGGTAGTTCACCTACGTGGACGTTCGGCCA26920.16885608782021969No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA25800.16183087168505456No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24210.15185757377888257No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24060.15091669661792295No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23480.14727863826221244No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23480.14727863826221244No Hit
GTCTGGGACAGACTTCAGTCTCACCATCAGCAGCCTGGAGCCTGAAGATT22540.14138247472019883No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22450.14081794842362305No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCGGCT20860.13084465051745106No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20820.1305937499411952No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCA20680.12971559792429954No Hit
CATCTATGGTGCATCCAGCCGGGCCACTGGCATCCCAGACAGGTTCAGTG20410.12802201903457222No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20180.1265793407211008No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20150.1263911652889089No Hit
GTTTATTACTGTGCGAGAGGGGGACCATATTACGATCTTTTCACTGGTTG20140.1263284401448449No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18820.11804872112840024No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18810.11798599598433626No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGAGAGCACTTAGCCTGGTACC18470.11585334108616115No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18140.11378341133204999No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17990.11284253417109037No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17990.11284253417109037No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17790.11158803128981086No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17680.11089805470510714No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCTCCCTTCTGTTGGTACCAGGCTAAGTGCTCTCTGCTAACACTCTGA17680.11089805470510714No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17320.10863994951880405No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTCGGTCCCTTGGCCGAACGTCCACGTAGGTGAACTACCAAACTG17190.10782452264597238No Hit
ATACAATATTATGTGGACTCTGTGACGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCTCTGTTTCTG16760.10512734145122149No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT16590.10406101400213391No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTCGGTCC16280.1021165345361507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG252.639568E-454.8914236
GGTATCA16250.041.1875951
ACCGATC3400.040.03284527
CGTATGC2400.038.91609241
GACCGAT3450.038.44580526
ATCTCCG3550.038.2667610
TATCTCG3000.037.7378536
TCGTATG2300.037.5353440
GTATCAA42350.036.6620181
TACGTAT901.5923077E-435.4275270
TATACAC4250.035.162613
CGATCAG4000.034.0739629
CCCACGA4000.033.97027619
CCGATCA4100.033.2167628
AGACCGA4100.032.33956525
TATCAAC48450.031.8258082
GCGAATA450.00396258431.64830657
CTTATAC4850.031.5227681
ATCTCGT3200.031.13621937
TCAACGC49650.031.0566024
Produced by FastQC (version 0.11.5)