FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594257
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTTTGGTAGTTCACCTACGTGGACGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAA55030.34517646778405237No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43170.27078444692417847No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42360.2657037102549965No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCA37080.2325848341892179No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31280.19620425063211264No Hit
GTGTTAGCAGAGAGCACTTAGCCTGGTACCAACAGAAGGGAGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCAT30330.19024536194603506No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28940.18152656692114258No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28050.17594402909944884No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26500.16622163176953278No Hit
GTGTATTACTGTCAGCAGTTTGGTAGTTCACCTACGTGGACGTTCGGCCA26420.16571983061702097No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24280.1522966497873304No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA24050.15085397147385898No Hit
GTCTGGGACAGACTTCAGTCTCACCATCAGCAGCCTGGAGCCTGAAGATT23410.14683956225376463No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23290.14608686052499692No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22550.1414451998642628No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21000.13172280253434673No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20840.13071920022932312No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCGGCT20610.1292765219158517No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20520.12871199561927593No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20010.12551301327201322No Hit
GTTTATTACTGTGCGAGAGGGGGACCATATTACGATCTTTTCACTGGTTG19140.12005592573844744No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCA18900.11855052228091205No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18790.11786054569620832No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18500.11604151651835307No Hit
CATCTATGGTGCATCCAGCCGGGCCACTGGCATCCCAGACAGGTTCAGTG18360.11516336450145741No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGAGAGCACTTAGCCTGGTACC18340.11503791421332947No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18240.11441066277268971No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17930.1124661833067065No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17920.11240345816264252No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17710.11108623013729906No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17600.11039625355259534No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17350.10882812495099597No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTCGGTCCCTTGGCCGAACGTCCACGTAGGTGAACTACCAAACTG17260.1082635986544202No Hit
CTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA17010.10669547005282085No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16890.10594276832405315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.041.300581
GTATCAA42050.036.9840551
TATCAAC46750.032.6683962
ATCAACG48750.031.1879433
TCAACGC48700.031.0825374
CAACGCA49300.030.8419385
AACGCAG49900.030.4701376
CGCAGAG52750.028.7595448
ACGCAGA52900.028.7421537
AGAGTAC52700.028.5873511
AATGGTA9150.028.29432370
GCGTTGT8550.027.84841762
GCAGAGT54550.027.6221759
TCTGGAG15050.026.23334970
CGTTGTC9250.025.82107563
CAGAGTA58750.025.6466810
GCCTAAT1801.1342967E-525.17015670
CGAAATT700.00123466324.97242748
TAAACGG9250.024.9504155
CCGTGTC9450.024.4208312
Produced by FastQC (version 0.11.5)