FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657817
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT86590.5223133795829094No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC85690.5168845536027198No Hit
GAGTTACAGTACCCCCTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT73230.44172547392142797No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC62420.3765192418704839No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC60220.36324877836335373No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA60060.3622836537446533No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA55280.33345055576097965No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC52750.31818952272777995No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52260.3152338285830101No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG52140.3145099851189848No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG50280.3032904114265929No Hit
GTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT50090.3021443259418862No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47770.2881500189707308No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTC46190.27861941336106455No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAGCTATTTAAATTGGTATCAGCAGAAA45530.2746382743089255No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT44610.2690888077513984No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG44280.26709823822532885No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT42640.25720571088365No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT42590.25690410944030617No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT42210.25461193847089275No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG41810.2521991269241418No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG41000.24731318354197115No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCCTCTTTTGG40730.24568453574791427No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAG40720.2456242154592455No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39760.23983346774704323No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCAAGATGACCCAGTC38830.23422368090084733No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38760.2338014388801659No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC38460.2319918302201027No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38220.23054414329205217No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG36190.21829912469229112No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC35220.2124480566914201No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT34130.2058731452265238No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCCTCTTTTGGCCAGGGGAC32740.19748862510156426No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA32400.195437735286826No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT31650.19091371363666798No Hit
GGCCAAAAGAGGGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAA31490.1899485890179676No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30940.18663097314118507No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG30060.181322787738333No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29820.17987510081028243No Hit
GAGTATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGCTGCATC29780.17963381965560735No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGAG29750.17945285878960102No Hit
GATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC29680.1790306167689196No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTA29520.17806549215021922No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA29390.17728132839752517No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28540.17215410386067945No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27860.16805232423120284No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26870.16208061565299428No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC26710.1611154910342939No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT25860.15598826649744815No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25720.15514378245608534No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCAAGATGACCCAGTCTCCATCCTCC25480.15369609552803476No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC25230.15218808831131542No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC25140.15164520571329645No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT25020.1509213622492712No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA25000.15080072167193362No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA24990.15074040138326486No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA24690.14893079272320164No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG24630.148568870991189No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24620.14850855070252023No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24210.14603541886710053No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23980.14464805222771873No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22830.1377112190308098No Hit
GTATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT22830.1377112190308098No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22620.13644449296876554No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22590.13626353210275924No Hit
CCCCTAAGGTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCA22550.13602225094808412No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22310.13457456402003357No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22140.13354911911266443No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT22100.13330783795798934No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22060.13306655680331425No Hit
GGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG21600.13029182352455065No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21600.13029182352455065No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC21520.12980926121520048No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21490.12962830034919415No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG21190.12781869168913096No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG20990.12661228591575546No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTTGATGT20570.124078833791667No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC20240.12208826426559746No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGT20190.1217866628222536No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20070.12106281935822832No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTG19830.11961513243017777No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT19760.11919289040949635No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19750.11913257012082758No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19670.11865000781147739No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTG19560.11798648463612088No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19310.11647847741940154No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG19030.1147895093366759No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18880.11388470500664429No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTA18880.11388470500664429No Hit
GTGCCAGATGTGACATCAAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA18630.11237669778992494No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18470.11141157317122456No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGA18100.10917972249047994No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18080.1090590819131424No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18030.10875748046979854No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC17960.10833523844911712No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17680.10664627036639147No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17660.10652562978905392No Hit
GCTAATACTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAG17480.105439864593016No Hit
ATCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATT17440.1051985834383409No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG17390.10489698199499704No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17300.10435409939697808No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17290.1042937791083093No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT17280.10423345881964052No Hit
GCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT16960.10230320958223979No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT16810.10139840525220818No Hit
AGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTC16720.10085552265418922No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16660.10049360092217659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3450.045.04402541
TCGTATG3350.044.23243740
GTGGTAT3400.042.57471
ATCTCGT3200.041.6573637
ATGCCGT3850.040.65987844
GTATCAA37500.039.5885281
TATCGCT350.001371374539.2908139
CTATCGC350.001392061739.17131438
TTATGCG350.001522570138.463534
CGAGACA4050.038.2653523
AATCTCG3550.037.48713336
ACGAGAC4150.036.5193522
CACGAGA4150.036.5138421
GTATGCC4650.034.99498742
CTTATAC4650.034.749641
TATCAAC42900.034.675462
CCGTCTT4550.034.6497547
TATACAC4800.034.3567473
TTATACA4900.033.655592
ATCTCCG4550.033.2807310
Produced by FastQC (version 0.11.5)